Effect of calcium and temperature on mixed lipid‐valinomycin monolayers A comparison of glycosphingolipids (ganglioside GT1b, sulphatides) and phosphatidylcholine

Schifferer, Florian; Beitinger, Heinz; Rahmann, H.; Möbius, Dietmar

doi:10.1016/0014-5793(88)81375-9

Cited by 21 publications

(7 citation statements)

References 12 publications

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“…In continuation of the Ca 2+ ‐binding experiments, variations of surface pressure‐area isotherms (= molecular space requirement; e.g., area per molecule; nm 2 ) caused by different temperatures and/or by the addition of Ca 2+ to the subphase of monolayers had been studied. 22,23 These were prepared from individual lipids (phosphatidylcholine (DOPC), sulfatide, G D1a , G D1b , G T1b ) and brain ganglioside extracts of summer‐ (22°C) versus winter‐ (−4°C) adapted dwarf hamsters, which show significant differences in their composition and hence polarity (%‐composition of major fractions at 22°C versus −4°C: G M1 : 16/13%; G D1a : 11/9%; G D1b : 17/14%; O‐Ac‐G T1b : 14/6%; G T1B : 27/30%; G Q1b : 7/20%).…”

Section: Physiochemical Evidence For Modified Reactivities Of Membmentioning

confidence: 99%

Differential Involvement of Gangliosides versus Phospholipids in the Process of Temperature Adaptation in Vertebrates: A Comparative Phenomenological and Physicochemical Study

Rahmann

Jonas

Kappel

et al. 1998

Annals of the New York Academy of Sciences

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The data presented support the idea that gangliosides are involved in thermal adaptation of neuronal membranes. Brain ganglioside patterns from cold-blooded vertebrate species living in different climates and from mammals during ontogenetical or seasonal changes in their body temperature were compared. The general rule "the lower the environmental temperature the more polar is the composition of brain gangliosides" as derived from these data was confirmed by the changes in ganglioside patterns evoked by experimentally induced cold acclimation of fish. To assess whether gangliosides are able to modulate the temperature-dependent properties of membranes, artificial mono- and bilayer membrane model systems were used. Incorporation of gangliosides in the model bilayer membranes evoked drastic changes in the dynamics of a peptide channel, suggesting that gangliosides are able to modulate basic membrane properties. In addition, data on thermosensitivity of ganglioside-calcium interactions and on surface behavior of gangliosides in monolayers are reviewed.

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Section: Physiochemical Evidence For Modified Reactivities Of Membmentioning

confidence: 99%

Differential Involvement of Gangliosides versus Phospholipids in the Process of Temperature Adaptation in Vertebrates: A Comparative Phenomenological and Physicochemical Study

Rahmann

Jonas

Kappel

et al. 1998

Annals of the New York Academy of Sciences

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“…For neutral phospholipid films it is indicated, that bivalent cations, especially Ca 2+ , do not exhibit an appreciable interaction (25). On the other hand there is a substantial interaction with monofilms of acidic phospholipids (25) and negatively charged glycosphingolipids (26,27,36,37).…”

Section: Resultsmentioning

confidence: 98%

“…This is in agreement with Maggio et al (33), that the introduction of negatively charged sialyl residues causes a decrease of the collapse pressure. Further details are described in two recent papers (36,37).…”

Section: Resultsmentioning

confidence: 99%

“…The properties of phospholipid, sulfatide, and ganglioside monolayers as well as their interaction with metal ions in the subphase, especially Ca 2+ , have been under intensive study by various groups of investigators (25)(26)(27)(30)(31)(32)(33)(34)(35)(36)(37). The work reported here is an extension of our previous studies (27,36,37), employing several naturally occurring glycosphingolipids, with emphasis on the influence of Ca 2+ on the individual molecular properties. In order to get more information about the possible functional role of the various acidic glycosphingolipids in the animal kingdom, in this paper for the first time comparative data are presented concerning the surface pressure and surface potential values of differently composed glycosphingolipids containing sialic acid (gangliosides G M i, G D i 0 , G T i b from vertebrates; compare Fig.…”

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confidence: 89%

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Comparative Monolayer Investigations of Surface Properties of Negatively Charged Glycosphingolipids from Vertebrates (Gangliosides) and Invertebrates (SGL-II, Lipid IV)

Beitinger

Schifferer

Sugita

et al. 1989

The Journal of Biochemistry

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The surface properties of four negatively charged glycosphingolipids from vertebrates, the sialo-glycosphingolipids (= gangliosides) GM1, GD1a, GT1b and a sulfo-glycosphingolipid (= sulfatide), and of the two negatively charged glycosphingolipids from lower invertebrates, the glucurono-glycosphingolipid Lipid IV and the aminophosphono-glycosphingolipid SGL-II were investigated in monolayers at the air/water interface. The molecular peculiarities under investigation were surface pressure (pi) and surface potential (delta V) which are described for Lipid IV and SGL-II for the first time. The surface pressure/area isotherms of all glycosphingolipids were typical of a liquid-expanded monolayer and, with the exception of SGL-II, exhibited a phase transition to a liquid-condensed state at surface pressures above 20 mN/m. The surface potential/molecular area data found for gangliosides in the closely packed state at pi = 30 mN/m (GM1: delta V = -17 mV; GD1a: delta V = -35 mV; GT1b: delta V = -39 mV) showed only a slight influence of the additional number of negatively charged residues. For Lipid IV, the surface behavior was very similar to GM1 both possessing one negative group per molecule, whereas in SGL-II also the surface potential data (delta V = +173 mV) were different compared with GD1a both possessing two negative groups per molecule. The addition of Ca2+ condensed the monolayers of all glycolipids and increased the potential in the direction to more positive values, but these findings were less effective in SGL-II films. On the basis of monolayer results presented here, in biological membranes of invertebrates especially Lipid IV might play a similar role as the ganglioside GM1 in vertebrate cells.

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“…[9][10][11][12] Ganglioside compositions are different among organs and/or tumors. 13) Ganglioside G M1 (GM1) and ganglioside G D1a (GD1a) are present in caveolae membrane, whereas ganglioside G T1b (GT1b) is not present in caveolae.…”

Section: A Trivalent Analogue Nnјnљ-tri-{5-[4-omethyl-b -D-glucopymentioning

confidence: 99%

Syntheses of New Model Compounds Related to an Antigenic Epitope from Bupleurum falcatum L. and Their Distributions in Various Ganglioside-Phospholipid Monolayers

Hada

Jin

Takeda

et al. 2006

CHEMICAL & PHARMACEUTICAL BULLETIN

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We synthesized 1) a trivalent analogue, N,NЈ,NЉ-tri-{5-[4-Omethyl-b -D-glucopyranosyluronic acid-(1→6)-b -D-galactopyranosyloxy]pentylcarbonylaminoethyl}-1,3,5-benzenetriamide (A) of b-D-GlcA4Me-(1→6)-b-D-Gal, which is related to a major antigenic epitope against antibupleurum 2IIc/PG-1-IgG from antiulcer pectic polysaccharide of Bupleurum falcatum L. (Japanese name Saiko), 2,3) and showed potent mitogenic activity and then previously showed 4) the AFM (atomic force microscopy) image of A in the ganglioside G M3 (GM3)/L-a-dipalmitoylphosphatidylcholine (DPPC) monolayer, where selective distribution of A in the GM3 region was found. Furthermore, we developed new peptidic glycoclusters and glycodentron, 5,6) and the fluorescence-labeledhave been synthesized for biological assay. Recently, we synthesized new glycocluster derivatives carrying double alkyl chains instead of fluorescent dansyl group. The distributions of the new derivatives, monomer type (1) and its clustering compound (2) in the GM3/DPPC monolayer have been examined using AFM, and the results have been promptly reported (Letters).8) (The details of synthesis have not been presented in the report 8) because of the limiting space.)Gangliosides are localized at the surface of mammalian membranes and participate in cellular interaction, differentiation and transformation.9-12) Ganglioside compositions are different among organs and/or tumors.13) Ganglioside G M1 (GM1) and ganglioside G D1a (GD1a) are present in caveolae membrane, whereas ganglioside G T1b (GT1b) is not present in caveolae.14) Membrane properties of GM1, GD1a and GT1b have been studied. [15][16][17] Studying drug distribution in biological membranes is interesting in how it relates to their pharmacological potency and is important to develop a convenient method for drug screening.In this study, the distributions of 1 and 2 in GM1/DPPC, GD1a/DPPC and GT1b/DPPC monolayers were observed using AFM. We report herein the results along with the syntheses of 1 and 2 (Fig. 1). Results and DiscussionSyntheses of Model Compounds Preparation of the designed trisaccharide monomer and tetramer derivative 1 and 2 were straightforward (Chart 1). Monomer amine derivative 3 and tetramer-cluster amine 6 were prepared according to the previous paper.6) 2-(Tetradecyl)hexadecanoic acid (4), was chosen as a fatty-alkyl residue. Coupling of amine derivatives 3 and 6 with 4 in the presence of diethyl phosphorocyanidate (DEPC) in dry DMF gave 5 (60%) and 7 (50%), respectively. Subsequent removal of all acyl groups and esters with sodium methoxide afforded the target compounds 1 and 2 in excellent yield.Distributions of 1 and 2 in the GM1/DPPC Monolayer First, the AFM image of the GM1/DPPC (4 : 6) monolayer at 37°C and 35 mN/m without 1 and 2 is shown in Fig. 2a. In Fig. 2a, the ratio of dark area to bright area is not 0.4, although the molar ratio of GM1 to DPPC is 0.4. This result Japan: and b Kyushu University of Health and Welfare; 1714-1 Yoshino-cho, Nobeoka, Miyazaki 882-8508, Japan. Received April 13, ...

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Effect of calcium and temperature on mixed lipid‐valinomycin monolayers A comparison of glycosphingolipids (ganglioside GT1b, sulphatides) and phosphatidylcholine

Cited by 21 publications

References 12 publications

Differential Involvement of Gangliosides versus Phospholipids in the Process of Temperature Adaptation in Vertebrates: A Comparative Phenomenological and Physicochemical Study

Differential Involvement of Gangliosides versus Phospholipids in the Process of Temperature Adaptation in Vertebrates: A Comparative Phenomenological and Physicochemical Study

Comparative Monolayer Investigations of Surface Properties of Negatively Charged Glycosphingolipids from Vertebrates (Gangliosides) and Invertebrates (SGL-II, Lipid IV)

Syntheses of New Model Compounds Related to an Antigenic Epitope from Bupleurum falcatum L. and Their Distributions in Various Ganglioside-Phospholipid Monolayers

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