Differential Involvement of Gangliosides versus Phospholipids in the Process of Temperature Adaptation in Vertebrates: A Comparative Phenomenological and Physicochemical Study

Rahmann, H.; Jonas, U.; Kappel, T.; Hildebrandt, Herbert

doi:10.1111/j.1749-6632.1998.tb09663.x

Cited by 16 publications

(18 citation statements)

References 33 publications

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“…Gangliosides have been hypothesized to play a role in thermal stabilization of the neuronal membrane, including adaptation of ion-channel function (Rahmann et al, 1998). We observed a slight tendency of the quantal content at dKO and GD3s-KO NMJs to increase with increasing temperature.…”

Section: Temperature-dependencymentioning

confidence: 56%

“…An alternative explanation for the tendency of an increase of quantal content at GD3s-KO and dKO NMJs at the higher temperatures tested could be an increased Ca 2+ availability. Gangliosides have temperature-dependent Ca 2+ binding sites of which the amount is positively associated with the extent of sialylation (Rahmann et al, 1998). Therefore, loss of sialic acid density in the vicinity of presynaptic voltage-gated Ca 2+ channels could lead to less adequate Ca 2+ buffering and thus to increased ACh release.…”

Section: Temperature-dependencymentioning

confidence: 99%

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Neuromuscular synaptic function in mice lacking major subsets of gangliosides

et al. 2008

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Section: Temperature-dependencymentioning

confidence: 56%

Section: Temperature-dependencymentioning

confidence: 99%

Neuromuscular synaptic function in mice lacking major subsets of gangliosides

et al. 2008

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“…A recent lipidomic analysis studying the lipid composition of the apical membrane of MDCK cells and of the envelope of influenza A viruses showed that the proportion of cholesterol and sphingolipids is enriched in the envelope of virions 24. The lipid composition of QT6 cells has not been described yet, but some studies on interspecies lipid analysis of various cells types and organs suggested differences between mammalian and avian cell bilayers 25,26. To point out these differences, the lipidomes of QT6 and MDCK cell bilayers are currently under analysis by gas chromatography–flame ionization detector.…”

Section: Discussionmentioning

confidence: 99%

Influenza A virus survival in water is influenced by the origin species of the host cell

Shigematsu

Dublineau

Olivier

et al. 2013

Influenza Resp Viruses

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BackgroundInfluenza A viruses have an envelope made of a lipid bilayer and two surface glycoproteins, the hemagglutinin and the neuraminidase. The structure of the virus is directly dependent on the genetic makeup of the viral genome except the glycosylation moieties and the composition of the lipid bilayer. They both depend on the host cell and are in direct contact with the environment, such as air or water. Virus survival is important for virus transmission from contaminated waters in the case of wild aquatic birds or from contaminated surface or air for humans.ObjectiveThe objective of this study was to check whether the origin species of the host cell has an influence on influenza A virus survival.MethodThe persistence in water at 35°C of viruses grown on either mammalian cells or avian cells and belonging to two different subtypes H1N1 and H5N1 was compared.ResultsBoth H5N1 and H1N1 viruses remained infectious for periods of time as long as 19–25 days, respectively. However, within the same subtype, viruses grown on mammalian cells were more stable in water at 35°C than their counterparts grown on avian cells, even for viruses sharing the same genetic background.ConclusionsThis difference in virus stability outside the host is probably connected to the nature of the lipid bilayer taken from the cell or to the carbohydrate side chains of the virus surface glycoproteins. Moreover, the long-lasting survival time might have a critical role in the ecology of influenza viruses, especially for avian viruses.

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“…Gangliosides may be involved in thermal adaptation of neuronal membrane function (Rahmann et al, 1998). We, therefore, performed synaptic electrophysiological measurements in a further series of experiments at 17, 20, 30, and 35 °C (Fig.…”

Section: Temperature-dependency Of Ach Release Parametersmentioning

confidence: 99%

“…Gangliosides have been suggested to play a role in the membrane flux of Ca 2+ and its cellular homeostasis (Ledeen and Wu, 2006;Rahmann et al, 1998;Wu et al, 2001). Therefore, we explored synaptic transmission at NMJs from muscle preparations incubated at low (0.2 mM) and high (5 mM) extracellular Ca 2+ , at 25 C bath temperature.…”

Section: Ca 2+ -Dependency Of Ach Release Parametersmentioning

confidence: 99%

Neuromuscular synaptic transmission in aged ganglioside-deficient mice

Zitman

Todorov

Verschuuren

et al. 2011

Neurobiology of Aging

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Gangliosides are sialylated glycosphingolipids that are present in high density on neuronal membranes, especially at synapses, where they are assumed to play functional or modulating roles. Mice lacking GM2/GD2-synthase express only the simple gangliosides GD3 and GM3 and develop progressive motor behaviour deficits upon ageing, apparently due to failing complex ganglioside-dependent maintenance and/or repair processes or, alternatively, toxic GM3/GD3 accumulation. We investigated the function of neuromuscular junctions (NMJs) of aged (>9 months-old) GM2/GD2-synthase null-mutant mice, because synaptic dysfunction might develop with age and could potentially contribute to the late-onset motor phenotype. In addition, we studied NMJs of old mice lacking GD3-synthase (expressing only O-and aseries gangliosides), which do not show an overt neurological phenotype but may develop subclinical synaptic deficits. Detailed electrophysiological analyses showed subtle changes in presynaptic neurotransmitter release. Acetylcholine release at 40 Hz nerve stimulation at aged GM2/GD2-synthase null-mutant NMJs ran down slightly more pronounced than at wild-type NMJs, and spontaneous acetylcholine release rate at GD3-synthase null-mutant NMJs was somewhat higher than at wild-type, selectively at 25 C bath temperature. Interestingly, we observed faster kinetics of postsynaptic electrophysiological responses at aged GD3-synthase null-mutant NMJs, not previously seen by us at NMJs of young GD3-synthase null-mutants or other types of (aged or young) ganglioside-deficient mice. These kinetic changes might reflect a change in postsynaptic acetylcholine receptor behaviour. Our data indicate that it is highly unlikely that transmission failure at NMJs contributes to the progressive motor defects of aged GM2/GD2-synthase null-mutants and that, despite some kinetic changes of synaptic signals, neuromuscular transmission remains successful in aged GD3-synthase null-mutant mice. Apparently, mutual redundancy of the different gangliosides in supporting presynaptic function, as observed previously by us in young mice, remains adequate upon ageing or, alternatively, gangliosides have only relatively little direct impact on neuromuscular synaptic function, even in aged mice. AbbreviationsACh, acetylcholine; AChR, acetylcholine receptor; EPP, endplate potential; GD3s-KO, α2,8-sialyltransferase knockout; GM2s-KO, β1,4-GalNAc-transferase knockout; MEPP, miniature endplate potential; NMJ, neuromuscular junction; WT, wild-type.Zitman et al., Synaptic function in aged ganglioside-deficient mice 3

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Differential Involvement of Gangliosides versus Phospholipids in the Process of Temperature Adaptation in Vertebrates: A Comparative Phenomenological and Physicochemical Study

Cited by 16 publications

References 33 publications

Neuromuscular synaptic function in mice lacking major subsets of gangliosides

Neuromuscular synaptic function in mice lacking major subsets of gangliosides

Influenza A virus survival in water is influenced by the origin species of the host cell

Neuromuscular synaptic transmission in aged ganglioside-deficient mice

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