Effect of calcium and chromium chelates of ethylenediaminetetraacetate on intestinal permeability and collagen metabolism in the rat

Aronson, Arthur L.; Rogerson, Katherine M.

doi:10.1016/0041-008x(72)90002-6

Cited by 28 publications

(5 citation statements)

References 26 publications

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“…Although 5"Cr-EDTA is ideal in that it is completely non-metabolised25 and administered in trace amounts, there remains the theoretical objection that membrane integrity could be compromised by the 06-1.8 ,mol EDTA26 27 (or Ca-EDTA28 29) employed to stabilise the test probe. Against this is the observation that in experimental animals, a concentration of at least 250 mmol EDTA is necessary before intestinal permeability becomes abnormal.26 27 Additionally, the results of the "Cr-EDTA absorption test closely resemble those of our in vitro studies where mucosal permeability was measured directly and independently of such variables as gastric emptying, intestinal transit and luminal dilutional factors.…”

Section: Discussionmentioning

confidence: 99%

Intestinal permeability in patients with coeliac disease and dermatitis herpetiformis.

Bjarnason¹,

Marsh²,

Price³

et al. 1985

Gut

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SUMMARY Intestinal permeability was investigated in patients with coeliac disease and dermatitis herpetiformis by a 5tChromium-labelled ethylenediaminetetraacetate (51Cr-EDTA) absorption test and the results correlated with histomorphometric analysis and intraepithelial lymphocyte counts of jejunal biopsies. The mean (±SD) 24 hour urine excretion of 51Cr-EDTA in 34 healthy volunteers was 1-9±O05% of the orally administered test dose. Patients with untreated coeliac disease (19) or untreated dermatitis herpetiformis (five) excreted significantly more 5tCr-EDTA than controls (5.9±2.7% and 4-6±2'1%, respectively, p<0.001) and all were outside the normal range of 1.O-2*6%. Patients with coeliac disease (42) treated for 6 months-23 years (mean 5 years) and patients with dermatitis herpetiformis (11) treated for 6 months-8 years (mean 3 years) excreted significantly more 5tCr-EDTA than controls, 4-2±2 4% p<0-0001 and 3.0O09% p<0003 respectively. Eleven of 14 (79%) treated patients with coeliac disease with an entirely normal jejunal mucosae demonstrated abnormal intestinal permeability. Intestinal permeability did not correlate significantly with either the mucosal height/crypt depth ratio or intraepithelial lymphocyte counts in jenunal biopsies from patients with untreated or treated coeliac disease.

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Section: Discussionmentioning

confidence: 99%

Intestinal permeability in patients with coeliac disease and dermatitis herpetiformis.

Bjarnason¹,

Marsh²,

Price³

et al. 1985

Gut

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“…Rats that did not have any gross appearance of blood in the urine had either unchanged or slightly increased amounts of amino acids characteristic of collagen present following Calcium EDTA infusion. The increase in proline excretion after Calcium EDTA infusion paralleled the increase in hydroxyprolin e excretion (Aronson and Rogerson 1972). Forland et al (1966) studied the renal excretion of EDTA in 38 dogs.…”

Section: Intravenousmentioning

confidence: 97%

“…The intestinal tract, especially the duodenum, of rats treated with Calcium EDTA appeared congested. The intestinal wall was thin, the lumen was lled with gas, and the intestinal weight was signi cantly less than that for control animals (Aronson and Rogerson 1972). Dogs (20) challenged twice daily with 150, 250, and 500 mg/ kg IV had LT 50 values of 40, 18.5, and 13 days, respectively (Bauer et al 1952).…”

Section: Intravenousmentioning

confidence: 99%

Final Report on the Safety Assessment of EDTA, Calcium Disodium EDTA, Diammonium EDTA, Dipotassium EDTA, Disodium EDTA, TEA-EDTA, Tetrasodium EDTA, Tripotassium EDTA, Trisodium EDTA, HEDTA, and Trisodium HEDTA

2002

Int J Toxicol

108

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EDTA (ethylenediamine tetraacetic acid) and its salts are substituted diamines. HEDTA (hydroxyethyl ethylenediamine triacetic acid) and its trisodium salt are substituted amines. These ingredients function as chelating agents in cosmetic formulations. The typical concentration of use of EDTA is less than 2%, with the other salts in current use at even lower concentrations. The lowest dose reported to cause a toxic effect in animals was 750 mg/kg/day. These chelating agents are cytotoxic and weakly genotoxic, but not carcinogenic. Oral exposures to EDTA produced adverse reproductive and developmental effects in animals. Clinical tests reported no absorption of an EDTA salt through the skin. These ingredients are likely, however, to affect the passage of other chemicals into the skin because they will chelate calcium. Exposure to EDTA in most cosmetic formulations, therefore, would produce systemic exposure levels well below those seen to be toxic in oral dosing studies. Exposure to EDTA in cosmetic formulations that may be inhaled, however, was a concern. An exposure assessment done using conservative assumptions predicted that the maximum EDTA dose via inhalation of an aerosolized cosmetic formulation is below that shown to produce reproductive or developmental toxicity. Because of the potential to increase the penetration of other chemicals, formulators should continue to be aware of this when combining these ingredients with ingredients that previously have been determined to be safe, primarily because they were not significantly absorbed. Based on the available data, the Cosmetic Ingredient Review Expert Panel found that these ingredients are safe as used in cosmetic formulations.

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“…The calcium chelate increases the permeability of the intestine and blood-brain barrier in the rat (1,3). The effect, therefore, may be a general one.…”

Section: Resultsmentioning

confidence: 97%

The effect of ethylenediaminetetraacetate and its calcium chelate on the rate of swelling of a strain of Pseudomonas aeruginosa in salt solutions

Bernheim¹

1972

Can. J. Microbiol.

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BERNHEIM, F. 1972. The effect of ethylenediaminetraacetate and its calciun~ chelate on the rateofswelling of a strain of Pseridomonas aerriginosa in salt solutions. Can. J. Microbiol. 18: 1643-1646. When washed suspensions of Pseudornonas aerrcginosa are placed in salt solutions, the cells shrink rapidly and then swell more slowly as salt and water enter them. The rate of swelling is increased by EDTA and its calcium chelate. The effect of EDTA is antagonized by diamines; the effect of the calcium chelate by carboxylic acids. The relative effects of EDTA and the calcium chelate differ in different salts which suggests different sites of action. The evidence indicates that EDTA does not act by chelating calcium or magnesium in the membrane of these cells but that its two nitrogen atoms combine by electrostatic forces with anionic sites, and the chelate, in which the nitrogen atoms are bound to calcium, combines by the same forces to cationic sites. Barium, strontium, and magnesium chelates are inactive.

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Effect of calcium and chromium chelates of ethylenediaminetetraacetate on intestinal permeability and collagen metabolism in the rat

Cited by 28 publications

References 26 publications

Intestinal permeability in patients with coeliac disease and dermatitis herpetiformis.

Intestinal permeability in patients with coeliac disease and dermatitis herpetiformis.

Final Report on the Safety Assessment of EDTA, Calcium Disodium EDTA, Diammonium EDTA, Dipotassium EDTA, Disodium EDTA, TEA-EDTA, Tetrasodium EDTA, Tripotassium EDTA, Trisodium EDTA, HEDTA, and Trisodium HEDTA

The effect of ethylenediaminetetraacetate and its calcium chelate on the rate of swelling of a strain of Pseudomonas aeruginosa in salt solutions

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