Effect of Cadmium on the Nucleoli of Meristematic Cells of Onion Allium cepa L: An Ultrastructural Study

Marcano, Letty; Carruyo, Ingrid; Campo, Antônio; Montiel, Xiomara

doi:10.1006/enrs.2001.4316

Cited by 27 publications

(11 citation statements)

References 14 publications

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“…This phenomenon is considered as an inducement to aneuploidy and polyploidy and has been reported as indicative of high toxicity [19,31,32]. Other anomalies were observed in groups exposed only to lead nitrate or to lead nitrate followed by ruthenium complex, including anaphasic bridges (Fig.…”

Section: Chromosomal Aberrations and Micronuclei Inductionmentioning

confidence: 84%

Cytotoxic and Genotoxic Effects of cis-Tetraammine(oxalato)Ruthenium(III) Dithionate on the Root Meristem Cells of Allium cepa

Pereira

Vilanova-Costa

Lima

et al. 2008

Biol Trace Elem Res

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Ruthenium complexes have attracted much attention as possible building blocks for new transition-metal-based antitumor agents. The present study examines the mitotoxic and clastogenic effects induced in the root tips of Allium cepa by cis-tetraammine(oxalato)ruthenium(III) dithionate {cis-[Ru(C(2)O(2))(NH(3))(4)](2)(S(2)O(6))} at different exposure durations and concentrations. Correlation tests were performed to determine the effects of the time of exposure and concentration of ruthenium complex on mitotic index (MI) and mitotic aberration index. A comparison of MI results of cis-[Ru(C(2)O(2))(NH(3))(4)](2)(S(2)O(6)) to those of lead nitrate reveals that the ruthenium complex demonstrates an average mitotic inhibition eightfold higher than lead, with the frequency of cellular abnormalities almost fourfold lower and mitotic aberration threefold lower. A. cepa root cells exposed to a range of ruthenium complex concentrations did not display significant clastogenic effects. Cis-tetraammine(oxalato)ruthenium(III) dithionate therefore exhibits a remarkable capacity to inhibit mitosis, perhaps by inhibiting DNA synthesis or blocking the cell cycle in the G2 phase. Further investigation of the mechanisms of action of this ruthenium complex will be important to define its clinical potential and to contribute to a novel and rational approach to developing a new metal-based drug with antitumor properties complementary to those exhibited by the drugs already in clinical use.

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Section: Chromosomal Aberrations and Micronuclei Inductionmentioning

confidence: 84%

Cytotoxic and Genotoxic Effects of cis-Tetraammine(oxalato)Ruthenium(III) Dithionate on the Root Meristem Cells of Allium cepa

Pereira

Vilanova-Costa

Lima

et al. 2008

Biol Trace Elem Res

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“…Cadmium may also interfere with RNA metabolism, trigger programmed cell death, cause chromatin fragmentation (Marcano et al 2002;Behboodi and Samadi 2004), and impact genetic functioning by affecting protein structure. Protein carbonylation (or oxidative protein modification) is commonly observed after Cd exposure (Kranner and Colville 2011).…”

Section: Cadmium Genotoxicitymentioning

confidence: 99%

Cadmium Bioavailability, Uptake, Toxicity and Detoxification in Soil-Plant System

Shahid

Dumat

Khalid

et al. 2016

Reviews of Environmental Contamination and Toxicology

227

175

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This review summarizes the findings of the most recent studies, published from 2000 to 2016, which focus on the biogeochemical behavior of Cd in soil-plant systems and its impact on the ecosystem. For animals and people not subjected to a Cd-contaminated environment, consumption of Cd contaminated food (vegetables, cereals, pulses and legumes) is the main source of Cd exposure. As Cd does not have any known biological function, and can further cause serious deleterious effects both in plants and mammalian consumers, cycling of Cd within the soil-plant system is of high global relevance.The main source of Cd in soil is that which originates as emissions from various industrial processes. Within soil, Cd occurs in various chemical forms which differ greatly with respect to their lability and phytoavailability. Cadmium has a high phytoaccumulation index because of its low adsorption coefficient and high soil-plant mobility and thereby may enter the food chain. Plant uptake of Cd is believed to occur mainly via roots by specific and non-specific transporters of essential nutrients, as no Cd-specific transporter has yet been identified. Within plants, Cd causes phytotoxicity by decreasing nutrient uptake, inhibiting photosynthesis, plant growth and respiration, inducing lipid peroxidation and altering the antioxidant system and functioning of membranes. Plants tackle Cd toxicity via different defense strategies such as decreased Cd uptake or sequestration into vacuoles. In addition, various antioxidants combat Cd-induced overproduction of ROS. Other mechanisms involve the induction of phytochelatins, glutathione and salicylic acid.

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“…Various stress responses, including those to heavy metals, induce anatomic and structural changes as well as physiological tolerance and/or adaptation in plant tissues. Moreover, studies of cadmium-associated changes in ultrastructure have mostly focused on organelles such as chloroplasts, mitochondria, nuclei (Djebali et al 2005;Vitória et al 2006), nucleoli (Marcano et al 2002), and plants (Vázquez et al 1992) exhibiting no differences in tolerance to cadmium. Previously, we have described selection of a tolerant cell line of Cucumis sativus L. and phytochelatin accumulation in sensitive and tolerant suspension cultures exposed to cadmium (Gzyl and Gwóźdź 2005).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructure analysis of cadmium-tolerant and -sensitive cell lines of cucumber (Cucumis sativus L.)

Gzyl

Przymusiński

Gwóźdź

2009

Plant Cell Tiss Organ Cult

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Previously, a stable cell suspension culture of cucumber tolerant to cadmium (Cd) was established (Gzyl and Gwóźdź, Plant Cell Tissue Organ Cult 80:59-67, 2005). In this study, ultrastructures of Cd-tolerant and -sensitive cells were analyzed by transmission electron microscopy (TEM). Ultrastructural differences between cell lines exposed to 100 lM CdCl 2 were observed both at cellular and organelle levels. Tolerant cells exposed to Cd exhibited well-preserved cellular structures in comparison with sensitive cells. Increased numbers of osmiophilic globules in the cytoplasm and nucleolus-associated bodies as well as electron dense material in vacuoles were observed in cadmium tolerant cells. In contrast, ultrastructure of sensitive cells following exposure to Cd exhibited distinct disturbances including vacuolation, disintegration of cytoplasm, and structural changes in both mitochondria and endoplasmic reticulum. TEM observations confirmed the adaptation of tolerant cells to Cd.

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Effect of Cadmium on the Nucleoli of Meristematic Cells of Onion Allium cepa L: An Ultrastructural Study

Cited by 27 publications

References 14 publications

Cytotoxic and Genotoxic Effects of cis-Tetraammine(oxalato)Ruthenium(III) Dithionate on the Root Meristem Cells of Allium cepa

Cytotoxic and Genotoxic Effects of cis-Tetraammine(oxalato)Ruthenium(III) Dithionate on the Root Meristem Cells of Allium cepa

Cadmium Bioavailability, Uptake, Toxicity and Detoxification in Soil-Plant System

Ultrastructure analysis of cadmium-tolerant and -sensitive cell lines of cucumber (Cucumis sativus L.)

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