Effect of cadmium and nickel on expression of CatSper 1 and 2 genes in mice

Mohammadi, Shabnam; Gholamin, Mehran; Mansouri, Atena; Mahmoodian, R.; Babazadeh, Beheshte; Kebriaei, S.M.; Zibaei, Behdad; Roshanaei, Mohammad; Daneshvar, F.; Khandehro, Mozhgan; Khodadadegan, Mohammad Amin; Delshad, Ali; Mohammadzadeh, Fatemeh; Mohammadi, Maryam; Sadeghi, Saeed; Shoeibi, Sara; Boroumand-Noughabi, Samaneh; Ghayour‐Mobarhan, Majid; Tavallaie, Shima; Vafaei, Azadeh; Ferns, Gordon A.

doi:10.1080/15569543.2017.1350192

Cited by 8 publications

(11 citation statements)

References 25 publications

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“…The most common types of morphological abnormalities were sperm head deformity (49.75%). The expression of CatSper 2 in the exposed group was significantly lower compared to the control group, while no significant change was observed for CatSper 1 (Mohammadi et al., 2018).…”

Section: Assessmentmentioning

confidence: 99%

Update of the risk assessment of nickel in food and drinking water

Schrenk¹,

Bignami²,

Bodin³

et al. 2020

EFS2

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The European Commission asked EFSA to update its previous Opinion on nickel in food and drinking water, taking into account new occurrence data, the updated benchmark dose (BMD) Guidance and newly available scientific information. More than 47,000 analytical results on the occurrence of nickel were used for calculating chronic and acute dietary exposure. An increased incidence of postimplantation loss in rats was identified as the critical effect for the risk characterisation of chronic oral exposure and a BMDL 10 of 1.3 mg Ni/kg body weight (bw) per day was selected as the reference point for the establishment of a tolerable daily intake (TDI) of 13 lg/kg bw. Eczematous flare-up reactions in the skin elicited in nickel-sensitised humans, a condition known as systemic contact dermatitis, was identified as the critical effect for the risk characterisation of acute oral exposure. A BMDL could not be derived, and therefore, the lowest-observed-adverse-effect-level of 4.3 lg Ni/kg bw was selected as the reference point. The margin of exposure (MOE) approach was applied and an MOE of 30 or higher was considered as being indicative of a low health concern. The mean lower bound (LB)/upper bound (UB) chronic dietary exposure was below or at the level of the TDI. The 95th percentile LB/UB chronic dietary exposure was below the TDI in adolescents and in all adult age groups, but generally exceeded the TDI in toddlers and in other children, as well as in infants in some surveys. This may raise a health concern in these young age groups. The MOE values for the mean UB acute dietary exposure and for the 95th percentile UB raises a health concern for nickel-sensitised individuals. The MOE values for an acute scenario regarding consumption of a glass of water on an empty stomach do not raise a health concern.

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Section: Assessmentmentioning

confidence: 99%

Update of the risk assessment of nickel in food and drinking water

Schrenk¹,

Bignami²,

Bodin³

et al. 2020

EFS2

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“…Sperm motility is also one of the important behaviors in fertilization. Intracellular calcium concentration not only plays a critical role in sperm movement, but also in the capacity of sperm and egg penetration [21,1]. The CatSper channel is a calcium channel in the mammalian sperm and contributes to the penetration of calcium into the spermatozoa, which is essential for the flagella waveform [22].…”

Section: Discussionmentioning

confidence: 99%

Astaxanthin Induces the Expression of CatSper1 Gene and Protects Sperms in Toxicity Induced by Cadmium in Mice

et al. 2021

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Cadmium (Cd) as a heavy metal damages testis and decreases fertility, however, antioxidants can improve sperm parameters and decrease male infertility. In this study we investigated the effect of astaxanthin (AST) on sperm parameters, expression levels of CatSper1 and CatSper2 genes in presence of Cd in mice. Thirty adults’ mice were divided into 4 groups, sham group received olive oil and saline (olive oil is the solvent of AST and saline is the solvent of Cd), Cd group received 1 mg/kg Cdcl2, a group received 10 mg/kg AST and 1 mg/kg Cdcl2 and a group received 10 mg/kg AST. The treatments were done intraperitoneally for 14 days. After 14 days sperm parameters were analyzed. Malondialdehyde level, catalase enzyme activity, the alteration of CatSper1 and CatSper2 genes expression were measured in testis. Results showed that Sperm count, viability, CatSper1 gene expression and catalase activity significantly decreased by Cd compared to sham group. Cd significantly increased sperm DNA fragmentation (SDF), abnormal sperm morphology and malondialdehyd level compared to sham group. AST significantly increased sperm count, viability and CatSper1 gene expression and decreased SDF and abnormal sperm in comparison with Cd group. AST protected testis and decreased oxidative stress induced by Cd. Our findings indicated that AST could protect sperm DNA, enhanced CatSper1 gene expression and sperm quality in presence of Cd. No significant differences were found in CatSper2 expression among treatments. Therefore, AST as a strong antioxidant can help to protect the potential of fertility against Cd toxicity.

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“…In a couple of studies performed by Mohammedi and colleagues on male mice treated with cadmium and nickel, there was a significant difference between the experimental and control groups regarding the expressions of CATSPER1 and CATSPER2 genes, which is not similar to our results. Aside from the toxic effects of selenium on most cells and tissues, it seems that it also has its undesirable effects on CATSPER1,2 gene and protein expression (22,23). In another study on adult male mice treated with L-carnitine, the expression rate of CATSPER1 protein and immunohistochemical reaction of the sperm (head, middle piece, and tail) increased in the experimental group compared to those in the control group.…”

Section: Discussionmentioning

confidence: 99%

Effect of experimental hyperthyroidism on CatSper1 and CatSper2 genes expression in the seminiferous tubules of BALB/c mice: An experimental study

Sadeghi

Jalali

Nikravesh

et al. 2020

IJRM

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Background: CATSPER 1 (Cation Channel Sperm Associated 1) and CATSPER2 channels have an important role in sperm motility. In this study, the effects of hyperthyroidism on Catsper1 and 2 genes of seminiferous tubules in mice testes were investigated. Objective: The present study was conducted to investigate the effect of hyperthyroidism on the expression of CATSPER1 and CATSPER2 genes in the seminiferous tubules of mice. Materials and Methods: This study was conducted on 20 BALB/C male mice divided into two groups - experimental and control. The experimental group was administered with 500 mg/l levothyroxine (L-thyroxine) liquid solution for two months for inducing hyperthyroidism, which was confirmed by radioimmunoassay. On the other hand, the control group was kept in animal houses under a normal condition. The implementation of real-time polymerase chain reaction and immunohistochemical studies was accomplished after the removal of the testes of the mice under anesthesia induced by chloroform. Results: Results showed that there was no significant difference in CATSPER1 (p = 0.45) and CATSPER2 (p = 0.34) gene expression between groups. At the same time, the color intensity showed no significant enhancement in the hyperthyroidism group (CATSPER1 p = 0.17 and CATSPER2 p = 0.22) as compared to the control group. Conclusion: Considering the key role of CATSPER in the molecular structure of the sperm, our findings showed that the hyperactivity of the thyroid gland has no significant effects on the function of these components. Therefore, it might be concluded that hyperthyroidism has no considerable effects on the seminiferous tubules. Key words: CATSPER1, CATSPER2, Hyperthyroidism, Mice, Sperm.

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Effect of cadmium and nickel on expression of CatSper 1 and 2 genes in mice

Cited by 8 publications

References 25 publications

Update of the risk assessment of nickel in food and drinking water

Update of the risk assessment of nickel in food and drinking water

Astaxanthin Induces the Expression of CatSper1 Gene and Protects Sperms in Toxicity Induced by Cadmium in Mice

Effect of experimental hyperthyroidism on CatSper1 and CatSper2 genes expression in the seminiferous tubules of BALB/c mice: An experimental study

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