Effect of Byrsonima crassa and Phenolic Constituents on Helicobacter pylori-Induced Neutrophils Oxidative Burst

Abstract

Byrsonima crassa Niedenzu (Malpighiaceae) is used in Brazilian folk medicine for the treatment of diseases related mainly to gastric ulcers. In a previous study, our group described the gastric protective effect of the methanolic extract from the leaves of B. crassa. The present study was carried out to investigate the effects of methanolic extract and its phenolic compounds on the respiratory burst of neutrophils stimulated by H. pylori using a luminol-based chemiluminescence assay as well as their anti-H. pylori activity. The suppressive activity on oxidative burst of H. pylori-stimulated neutrophils was in the order of methyl gallate > (+)-catechin > methanol extract > quercetin 3-O-α-l-arabinopyranoside > quercetin 3-O-β-d-galactopyranoside > amentoflavone. Methyl gallate, compound that induced the highest suppressive activity with IC50 value of 3.4 μg/mL, did not show anti-H. pylori activity. B. crassa could be considered as a potential source of natural antioxidant in gastric ulcers by attenuating the effects on the damage to gastric mucosa caused by neutrophil generated reactive oxygen species, even when H. pylori displays its evasion mechanisms.

Comparison of Brazilian Plants Used to Treat Gastritis on the Oxidative Burst ofHelicobacter pylori-Stimulated Neutrophil

“…The differences in the chemical constituents as well as in the quantity of several components might justify differences in the antioxidant activity. Bonacorsi et al [29], under the same working conditions used in this study, reported the inhibition of luminol oxidation on neutrophil oxidative burst generated by H. pylori by some phenolic compounds. At 5  μ g/mL concentration, the most potent inhibitor was methyl gallate (73.1%) compared to (+)-catechin (28.3%), amentoflavone (16.7%), quercetin 3-O-alpha-L-arabinopyranoside (8.9%), and quercetin 3-O-beta-D-galactopyranoside (7.2%).…”

“…The bacterium was grown in a microaerophilic atmosphere at 37°C on Columbia agar containing 5% sheep blood for 3 days. H. pylori organisms were collected and suspended in 0.01 M phosphate buffered 0.15 M saline, pH 7.4 (PBS) at different absorbance (0.15, 0.2, and 0.3) with a 620 nm filter in order to follow the respiratory burst of polymorphonuclear neutrophils exposed to bacteria using luminol-enhanced chemiluminescence assay performed following the protocol described by Bonacorsi et al [29]. Briefly, 5.0 × 10 6  cells/mL and 2.0 × 10 −5  M luminol (Sigma Chemical Co., St. Louis, MO) were added to a tube containing PBS-D.…”

Natural Products for the Prevention and Management of Helicobacter pylori Infection

“…It was demonstrated that 5-methoxy-3,4dehydroxanthomegnin from Paepalanthus latipes (Kitagawa, Bonacorsi, Da Fonseca, Vilegas, & Raddi, 2012), and the methanol extracts of Qualea parviflora and Qualea multiflora (Bonacorsi, Da Fonseca, Raddi, Kitagawa, & Vilegas, 2013) could inhibit H. pylori-induced oxidative burst in rats, and the latter two showed activities at low concentrations (5 μg/mL). In another study, methyl gallate from Byrsonima crassa showed the highest inhibitory effects on oxidative burst of H. pylori-stimulated neutrophils (IC 50 : 3.4 μg/mL), among all the phenolic compounds and methanol extract from B. crassa (Bonacorsi, Raddi, Da Fonseca, Sannomiya, & Vilegas, 2012). Moreover, Achillea millefolium, Berberis aristata, Coriandrum sativum, Foeniculum vulgare, Matricaria chamomilla, and Prunus domestica have been illustrated to significantly inhibit the ROS generation in H. pylori-infected cells (Zaidi et al, 2012).…”

Bioactive phenolic compounds, metabolism and properties: a review on valuable chemical compounds in Scots pine and Norway spruce

“…Quercetin has exhibited antibacterial activity against M. luteus, B. subtilis, S. aureus, S. epidermidis, E. coli, P. aeruginosa (Rauha et al 2000), and H. pylori (Martini et al 2009;Bonacorsi et al 2012). MIC values of 604 mg L -1 against S. aureus (Ibewuike et al 1997) and MBC of\ 200 mg L -1 against H. pylori (Martini et al 2009) have been reported.…”