Effect of Bursectomy and Thymectomy on the Responses of Chicken Peripheral Blood Lymphocytes to Phytohaemagglutinin

Mf, Greaves; Im, Roitt; Rose, Marie E.

doi:10.1038/220293a0

Cited by 118 publications

(33 citation statements)

References 15 publications

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“…PHA-induced in vitro lymphocyte stimulation has been used to assess the immune capabilities of these cells (11,12,30,31). When lymphocytes are cultured in the presence of PHA there is a sequential stimulation of RNA, protein, and DNA synthesis.…”

Section: Resultsmentioning

confidence: 99%

DNA polymerase activity as an index of lymphocyte stimulation: studies in Down's syndrome

Ss¹,

Bs²,

Bj³

et al. 1970

J. Clin. Invest.

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Section: Resultsmentioning

confidence: 99%

DNA polymerase activity as an index of lymphocyte stimulation: studies in Down's syndrome

Ss¹,

Bs²,

Bj³

et al. 1970

J. Clin. Invest.

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“…LAF was active on preparations freed of macro-phages by adherence to plastic or nylon fibers. It potentiated responses to PHA and to suboptimal concentrations of soluble Con A, mitogens which stimulate T lymphocytes exclusively (, [39][40][41][42][43]. The failure of PHA to stimulate spleen cells of thvmectomized XBM mice, even a month after reconstitution (33), showed that LAF and PHA are not active on B lymphocytes.…”

Section: Discussionmentioning

confidence: 99%

Potentiation of the T-Lymphocyte Response to Mitogens

Gery

Gershon

Waksman

1972

The Journal of Experimental Medicine

792

151

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Human and mouse lymphoid cells, stimulated by phytohemagglutinin (PHA) or lipopolysaccharide W (LPS), release supernatant factor(s) which are mitogenic for mouse thymocytes and which potentiate their responses to PHA or concanavalin A (Con A), The term LAF (lymphocyte-activating factor) is proposed for this activity. LAF not only enhances the mitotic responses of the less dense thymus subpopulations (A, B, and C) separable on discontinuous bovine serum albumin (BSA) gradients but also gives substantial responses in the otherwise inert cells of the denser fractions D and P. LAF does not exert a potentiating stimulatory effect on the responses of unfractionated mouse spleen cells, but does act synergistically with PHA on nonadherent spleen cells and on spleen cells of mice of several strains 5 days after irradiation and injection of thymocytes. Similarly LAF, which has no visible effect on unfractionated human peripheral blood cells, strongly potentiates the PHA response of column-purified lymphocytes, when these are cultured at low concentration. We conclude that LAF stimulates both central and peripheral T lymphocytes and enhances their responses to other stimulants.

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“…Are the cells which respond to stimulation with PHA the same cells which manifest cellular immunity in response to certain types of antigens, such as the trans- plantation antigens. Clinical and experimental observations indicate that the ability to respond to PHA and participate in reactions of cellular immunity requires an intact functioning thymus (20)(21)(22)(23). In a previous communication, it was established that the thymus cells are capable of responding to stimulation with PHA and allogeneic leukocytes, but not to GARIG (1).…”

Section: Discussionmentioning

confidence: 99%

Cells Involved in the Immune Response

Daguillard

Richter

1970

The Journal of Experimental Medicine

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In a preceding article, evidence was presented for the existence of two distinct populations of lymphocytes in the normal adult r a b b i t --o n e capable of responding to stimulation with phytohemagglutinin (PHA) 1 and the other to goat antiserum to rabbit immunoglobulins (1). The question which these results pose is whether one or both of these functionally different populations of cells are capable of reacting to common protein antigens. This invesigation was designed to provide the answer to this question. The results clearly imply that only the lymphocytes which react to anti-immunoglobulin antiserum react to stimulation with specific antigen, with both of these reagents probably interacting with the same receptor site on the lymphocyte surface.

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Effect of Bursectomy and Thymectomy on the Responses of Chicken Peripheral Blood Lymphocytes to Phytohaemagglutinin

Cited by 118 publications

References 15 publications

DNA polymerase activity as an index of lymphocyte stimulation: studies in Down's syndrome

DNA polymerase activity as an index of lymphocyte stimulation: studies in Down's syndrome

Potentiation of the T-Lymphocyte Response to Mitogens

Cells Involved in the Immune Response

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