Effect of Buparvaquone on Cryptosporidium parvum Oocysts Shedding in Calf

Alidadi, Nasser; Dezfouli, Mohammad Reza Mokhber; Rahbari, Sedegh; Nouri, Mohammad; Ali, Abdulghayoum Mirza; Bahonar, Alirezaا; Bulurchia, Mahmud

doi:10.3923/ajava.2008.275.277

Cited by 6 publications

(3 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although there are no comprehensive studies in the literature regarding the use of buparvaquone in C. parvum infections, Alidadi et al (2008) investigated the effect of the buparvaquone on oocyst density in calves with cryptosporidiosis and found that buparvaquone significantly reduced oocyst scattering. But these researchers applied isotonic saline to the calves in the control group and did not evaluate the clinical improvement.…”

Section: Discussionmentioning

confidence: 99%

“…The drug has also been used in combination with azithromycin to treat Babesia vulpes infection in dogs (Checa et al, 2017) and to treat Sporothrix brasiliensis infection in cats (Borba-Santos et al, 2021). To the best of our knowledge, there is only one study investigating the effect of buparvaquone against cryptosporidiosis, but that study solely evaluated oocyst shedding as a parameter (Alidadi et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of the effects of buparvaquone and paromomycin on oocyst excretion and clinical parameters in diarrheal calves naturally infected with cryptosporidiosis

Evci

Şentürk

2023

J Hellenic Vet Med Soc

View full text Add to dashboard Cite

Cryptosporidium is an obligate intracellular parasite that infects a wide variety of hosts. Twenty calves with cryptosporidiosis were divided into two groups. A single dose of 2.5 mg/kg buparvaquone was administered intramuscularly to one group, while 100 mg/kg/day paromomycin was administered orally to the other group. The results obtained from the study in which oocyst shedding and clinical parameters of sucking reflex, mental status, oocyst scattering were observed in both groups offer an effective control option with this study, which provides results that support the application of a single dose of buparvaquone injection against the complex treatment methods applied to calves with cryptosporidiosis in the field. In this study, a significant decrease in oocyst shedding and rapid improvement in clinical findings were observed in calves.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of the effects of buparvaquone and paromomycin on oocyst excretion and clinical parameters in diarrheal calves naturally infected with cryptosporidiosis

Evci

Şentürk

2023

J Hellenic Vet Med Soc

View full text Add to dashboard Cite

show abstract

“…Buparvaquone (BPQ), a hydroxynaphthoquinone derivative, has been investigated for the treatment of many veterinary infections including leishmaniasis, pneumonia, cryptosporidiosis, equine piroplasmosis and is recommended as the gold standard for the treatment of theileriosis. [ 1 2 3 4 ] Mortality in untreated animals is very high. Moreover, almost all the recovered animals remain carriers for life, with growth and production potential never achieved.…”

mentioning

confidence: 99%

Buparvaquone loaded solid lipid nanoparticles for targeted delivery in theleriosis

et al. 2014

View full text Add to dashboard Cite

Background:Buparvaquone (BPQ), a hydroxynaphthoquinone derivative, has been investigated for the treatment of many infections and is recommended as the gold standard for the treatment of theileriosis. Theileriosis, an intramacrophage infection is localized mainly in reticuloendotheileial system (RES) organs. The present study investigates development of solid lipid nanoparticles (SLN) of BPQ for targeted delivery to the RES.Materials and Methods:BPQ SLN was prepared using melt method by adding a molten mixture into aqueous Lutrol F68 solution (80°C). Larger batches were prepared up to 6 g of BPQ with GMS: BPQ, 2:1. SLN of designed size were obtained using ultraturrax and high pressure homogenizer. A freeze and thaw study was used to optimize type and concentration of cryoprotectant with Sf: Mean particle size, Si: Initial particle size <1.3. Differential scanning calorimetry (DSC), powder X-ray diffraction (XRD) and scanning electron microscope (SEM) study was performed on optimized formulation. Formulation was investigated for in vitro serum stability, hemolysis and cell uptake study. Pharmacokinetic and biodistribution study was performed in Holtzman rat.Results:Based on solubility in lipid; glyceryl monostearate (GMS) was selected for preparation of BPQ SLN. Batches of BPQ SLN were optimized for average particle size and entrapment efficiency at <100 mg solid content. A combination of Solutol HS-15 and Lutrol F68 at 2% w/v and greater enabled the desired Sf/Si < 1.3. Differential scanning calorimetry and powder X-ray diffraction revealed decrease in crystallinity of BPQ in BPQ SLN while, scanning electron microscope revealed spherical morphology. BPQ SLN revealed good stability at 4°C and 25°C. Low hemolytic potential (<8%) and in vitro serum stability up to 5 h was observed. Cytotoxicity of SLN to the U937 cell was low. The macrophage cell line revealed high (52%) uptake of BPQ SLN in 1 h suggesting the potential to RES uptake. SLN revealed longer circulation and biodistrbution study confirmed high RES uptake (75%) in RES organs like liver lung spleen etc.Conclusion:The high RES uptake suggests BPQ SLN as a promising approach for targeted and improved delivery in theileriosis.

show abstract

Spectrofluorimetric determination of buparvaquone in biological fluids, food samples and a pharmaceutical formulation by using terbium–deferasirox probe

Manzoori¹,

Jouyban²,

Amjadi³

et al. 2011

Food Chemistry

View full text Add to dashboard Cite

Effect of Buparvaquone on Cryptosporidium parvum Oocysts Shedding in Calf

Cited by 6 publications

References 3 publications

Comparison of the effects of buparvaquone and paromomycin on oocyst excretion and clinical parameters in diarrheal calves naturally infected with cryptosporidiosis

Comparison of the effects of buparvaquone and paromomycin on oocyst excretion and clinical parameters in diarrheal calves naturally infected with cryptosporidiosis

Buparvaquone loaded solid lipid nanoparticles for targeted delivery in theleriosis

Spectrofluorimetric determination of buparvaquone in biological fluids, food samples and a pharmaceutical formulation by using terbium–deferasirox probe

Contact Info

Product

Resources

About