Effect of buffalo seminal plasma heparin binding protein (HBP) on freezability and in vitro fertility of buffalo cauda spermatozoa

Harshan, Hiron M.; Singh, L. P.; Arunachalam, A.; Ansari, M.R.; Kumar, Satish

doi:10.1016/j.anireprosci.2005.07.010

Cited by 32 publications

(25 citation statements)

References 26 publications

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“…Rodriguez et al (2011) did not, also, find changes in bovine semen progressive motility after heparin induced capacitation. In agreement with our results, Harshan et al (2006) reported a significant increase of buffalo bull epididymal semen motility after incubation with heparin (20 μg/ml, 20 min, 37 °C).…”

Section: Discussionsupporting

confidence: 82%

Evaluation of common in vitro used chemicals' effect on motility and chromatin stability of boar spermatozoa

Tsakmakidis

Khalifa²,

Lymberopoulos

et al. 2017

J Hellenic Vet Med Soc

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Chemicals such as heparin, calcium ionophore and dimethyl sulfoxide (DMSO) have been used in sperm's function evaluation assays, since they are facilitating the sperm capacitation and the induction of acrosome reaction. Many researchers modified porcine in vitro protocols purposing the improvement and validation of in vitro assays. The modification of the above mentioned chemicals' concentration could have a detrimental effect on sperm characteristics, relative to normal sperm function and fertilizing ability. The present study aimed to investigate in vitro effect of various concentrations of, a) heparin (2, 4, 6, 8 and 10 μg/ml), b) calcium ionophore A23187 (10, 20, 30 μΜ) and c) DMSO (1, 2, 3 % v/v), that have been used in sperm evaluation techniques, on chromatin instability and total motility of boar spermatozoa. Eight boars were used as semen donors, providing 20 ejaculates. Acridine orange test and subjective evaluation of semen by a microscope equipped with a heated plate were performed in order to evaluate chromatin integrity and motility, respectively. The results of this study showed that the addition of heparin, A23187 and DMSO at concentrations of 8 - 10 μ^πιΐ, 10 μΜ and 1%, respectively, can be used for in vitro handling of boar spermatozoa without reduction of their motility and chromatin quality.

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Section: Discussionsupporting

confidence: 82%

Evaluation of common in vitro used chemicals' effect on motility and chromatin stability of boar spermatozoa

Tsakmakidis

Khalifa²,

Lymberopoulos

et al. 2017

J Hellenic Vet Med Soc

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“…However, increase in only motility and HOST due to supplementation of SP-HBP was significant (p<0.05) at both phases of cryopreservation. Harshan et al (2006) revealed that 40 μg dose of HBP improved the motility, HOST and cervical mucus penetration test at pre-freeze phase of buffalo semen cryopreservation, but they did not observe positive effect of this dose on frozen-thawed semen. Barrios et al (2000) also demonstrated that the absorption of isolated proteins from seminal plasma may reduce the cold shock effect.…”

Section: Resultsmentioning

confidence: 85%

“…Manjunath et al (2002) also suggested that at a greater concentration of bovine seminal plasma proteins, more cholesterol and phospholipids are removed from the sperm membrane, resulting in its destabilization that decreases sperm resistance to cold shock and freezing. Harshan et al (2006) also postulated that higher loss of semen parameters with higher amount of HBP could be due to an increased cholesterol efflux in the spermatozoa which led to more pronounced cryo-damage. Therefore, effects of 20 μg/mL of SP-HBP on semen cryopreservation of six bulls were evaluated.…”

Section: Resultsmentioning

confidence: 99%

Seminal Plasma Heparin Binding Proteins Improve Semen Quality by Reducing Oxidative Stress during Cryopreservation of Cattle Bull Semen

Patel

Gandotra

Cheema

et al. 2015

Asian Australas. J. Anim. Sci

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Heparin binding proteins (HBPs) are produced by accessory glands. These are secreted into the seminal fluid, bind to the spermatozoa at the time of ejaculation, favour capacitation, acrosome reaction, and alter the immune system response toward the sperm. The present study was conducted with an objective to assess the effect of purified seminal plasma-HBPs (SP-HBPs) on cross bred cattle bull sperm attributes during two phases of cryopreservation: Pre freezing and freezing-thawing. SP-HBPs were purified from pooled seminal plasma by heparin affinity chromatography. Three doses of SP-HBPs i.e. 10, 20, 40 μg/mL semen were standardized to find out the optimum dose and 20 μg/mL was found to be an optimum dose. Semen as such and treated with SP-HBPs was diluted with sodium citrate-egg yolk diluter and cryopreserved as per the standard protocol. Sperm parameters i.e. motility, viability, Hypo-osmotic swelling test (HOST), acrosome damage, in vitro capacitation and lipid peroxidation were evaluated in SP-HBP treated and untreated (control) semen at both phases of cryopreservation. A considerable variation in percent sperm motility, viability, membrane integrity (HOST), acrosome damage, acrosome reaction and lipid peroxidation was observed at both phases among the bulls irrespective of the treatment. Incubation of neat semen with 20 μg/mL SP-HBP before processing for cryopreservation enhanced the average motility, viability, membrane integrity by 7.2%, 1.5%, 7.9%, and 5.6%, 6.6%, 7.4% in pre-frozen and frozen-thawed semen in comparison to control. There was also an average increase of 4.1%/3.9% in in vitro capacitation and acrosome reaction in SP-HBPs-treated frozen-thawed semen as compared to control. However, binding of SP-HBPs to the sperm declined acrosome damage and lipid peroxidation by 1.3%/4.1% and 22.1/32.7 μM/109 spermatozoa in SP-HBP treated pre-frozen/frozen-thawed semen as compared to control, respectively. Significant (p<0.05) effects were observed only in motility, HOST and in vitro acrosome reaction. It can be concluded that treatment of neat semen with SP-HBPs before cryopreservation minimized the cryoinjury by decreasing the generation of reactive oxygen species.

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“…In buffalo, heparin and gelatin binding seminal plasma proteins improved functional membrane integrity and the ability to penetrate the cervical mucus of cauda epididymal spermatozoa (Arangasamy et al 2005;Kumar et al 2008). However, concentrations of heparin-binding protein and protein with N-terminus aspartic acid, D, and carboxy terminus cystine, having 109 amino acids (PDC-109) were positively correlated with cryodamage and affected the freezability of buffalo spermatozoa (Harshan et al 2006;Singh et al 2014). The role of other buffalo seminal plasma proteins in regulating fertility has not been studied in detail.…”

Section: Introductionmentioning

confidence: 99%

Relationship between seminal plasma tuberoinfundibular peptide of 39 residues and sperm functional attributes in buffalo (Bubalus bubalis)

Selvaraju

Somashekar

Krishnan

et al. 2016

Reprod. Fertil. Dev.

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The buffalo seminal plasma protein profile and its relationship with sperm quality have not been studied in detail. Thus, the aim of the present study was to profile buffalo seminal plasma proteins and to assess the relationship between differentially expressed proteins and sperm characteristics. Semen samples (n = 44) were collected from 11 Murrah buffalo bulls (four ejaculates from each animal) and seminal plasma protein profiling was performed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Matrix-assisted laser desorption ionisation time-of-flight analysis of one of the differentially expressed proteins, namely the 11-12 kDa protein, identified it as tuberoinfundibular peptide of 39 residues (TIP39). Western blot analysis confirmed the presence of TIP39, with TIP39 expression in seminal plasma varying among bulls. Based on TIP39 levels, bulls were classified into two groups, those with high and low protein. The percentages of spermatozoa positive for mitochondrial membrane potential test, chromatin distribution test, synthetic media sperm penetrability test and acrosomal integrity test were significantly (P < 0.05) high in the high protein group. The present study is the first to demonstrate the presence of TIP39 in buffalo seminal plasma and the positive effect of TIP39 on the functional parameters and fertilising ability of spermatozoa.

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Effect of buffalo seminal plasma heparin binding protein (HBP) on freezability and in vitro fertility of buffalo cauda spermatozoa

Cited by 32 publications

References 26 publications

Evaluation of common in vitro used chemicals' effect on motility and chromatin stability of boar spermatozoa

Evaluation of common in vitro used chemicals' effect on motility and chromatin stability of boar spermatozoa

Seminal Plasma Heparin Binding Proteins Improve Semen Quality by Reducing Oxidative Stress during Cryopreservation of Cattle Bull Semen

Relationship between seminal plasma tuberoinfundibular peptide of 39 residues and sperm functional attributes in buffalo (Bubalus bubalis)

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