Relationship between seminal plasma tuberoinfundibular peptide of 39 residues and sperm functional attributes in buffalo (Bubalus bubalis)

Abstract

The buffalo seminal plasma protein profile and its relationship with sperm quality have not been studied in detail. Thus, the aim of the present study was to profile buffalo seminal plasma proteins and to assess the relationship between differentially expressed proteins and sperm characteristics. Semen samples (n = 44) were collected from 11 Murrah buffalo bulls (four ejaculates from each animal) and seminal plasma protein profiling was performed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Matrix-assisted laser desorption ionisation time-of-flight analysis of one of the differentially expressed proteins, namely the 11-12 kDa protein, identified it as tuberoinfundibular peptide of 39 residues (TIP39). Western blot analysis confirmed the presence of TIP39, with TIP39 expression in seminal plasma varying among bulls. Based on TIP39 levels, bulls were classified into two groups, those with high and low protein. The percentages of spermatozoa positive for mitochondrial membrane potential test, chromatin distribution test, synthetic media sperm penetrability test and acrosomal integrity test were significantly (P < 0.05) high in the high protein group. The present study is the first to demonstrate the presence of TIP39 in buffalo seminal plasma and the positive effect of TIP39 on the functional parameters and fertilising ability of spermatozoa.

Comparative sperm protein profiling in bulls differing in fertility and identification of phosphatidylethanolamine‐binding protein 4, a potential fertility marker

“…An ejaculate having less percentage of total motile spermatozoa may fail to reach the site of fertilization due to lack of motility regulating factors secreted in epididymis (Fu-Jun & Xiao-Fang, 2012;Yoon et al, 2016) and proteins such as CatSper, IGF1, and ODF1 (Tamburrino et al, 2014;Cabrillana et al, 2017;Selvaraju et al, 2016b) BSP1 (Somashekar et al, 2015), and TIP39 (Selvaraju et al, 2016a). An ejaculate having less percentage of total motile spermatozoa may fail to reach the site of fertilization due to lack of motility regulating factors secreted in epididymis (Fu-Jun & Xiao-Fang, 2012;Yoon et al, 2016) and proteins such as CatSper, IGF1, and ODF1 (Tamburrino et al, 2014;Cabrillana et al, 2017;Selvaraju et al, 2016b) BSP1 (Somashekar et al, 2015), and TIP39 (Selvaraju et al, 2016a).…”

“…In the present study, total sperm motility, percentage of type-A sperm, sperm acrosome reaction, sperm fructose uptake, and sperm intracellular calcium signaling were significantly higher in fertile group than the low fertile and infertile groups. An ejaculate having less percentage of total motile spermatozoa may fail to reach the site of fertilization due to lack of motility regulating factors secreted in epididymis (Fu-Jun & Xiao-Fang, 2012;Yoon et al, 2016) and proteins such as CatSper, IGF1, and ODF1 (Tamburrino et al, 2014;Cabrillana et al, 2017;Selvaraju et al, 2016b) BSP1 (Somashekar et al, 2015), and TIP39 (Selvaraju et al, 2016a). The low percentages of acrosomal reacted spermatozoa of low and infertile groups might suggest the inability of spermatozoa to undergo acrosome exocytosis and lack of calcium-mediated signaling cascades further affects penetration of zona pellucida (Tranter et al, 2000).…”

Shotgun Proteomics-based distinctive fingerprint of buffalo sperm surface unravels over-representation of proteins driving spermatogenesis, motility, and fertility

“…Moreover, since the fertility of thousands of buffaloes is at stake by a single bull, it is therefore imperative to elucidate the buffalo sperm surface pro le and their molecular functions that can help to understand their role vis-à-vis male fertility. Although a handful of studies are available on buffalo sperm proteome, these contemporary studies exhibit an inclination towards either seminal plasma proteins or biomarker discovery [33][34][35][36][37]. The proteomics analysis of semen is considered complicated since it contains secretions from various accessory glands and its proteome varies between and within individuals and exhibits seasonal and temporal uctuations [20,38].…”

Shotgun Proteomics-based distinctive fingerprint of sperm-surface revealed over- representation of proteins driving spermatogenesis, motility, and fertility

“…Moreover, since the fertility of thousands of buffaloes is at stake by a single bull, it is therefore imperative to elucidate the buffalo sperm surface pro le and their molecular functions that can help to understand their role vis-à-vis male fertility. Although a handful of studies are available on buffalo sperm proteome, these contemporary studies exhibit an inclination towards either seminal plasma proteins or biomarker discovery [33][34][35][36][37]. The proteomics analysis of semen is considered complicated since it contains secretions from various accessory glands and its proteome varies between and within individuals and exhibits seasonal and temporal uctuations [20,38].…”