Effect of Bone Marrow Versus Adipose Tissue Derived Mesenchymal Stem Cells on the Pancreas of Streptozotocin-Induced Diabetes Mellitus Type I in Adult Male Rats (Histological Study)

Abstract

Background: Diabetes Mellitus (D.M.) is a major health problem affecting more than 200 million worldwide. The ideal treatment for autoimmune type I diabetes is regeneration of endogenous β-cells which could be achieved by mesenchymal stem cells transplantation. Aim of the work:This work aimed to compare the effect of intravenous bone marrow derived mesenchymal stem cells (BMSCs) and adipose tissue derived mesenchymal stem cells (AMSCs) on Streptozotocin (STZ)-induced type I diabetes in albino rats. Material and Methods: Fifty albino male rats were divided into 4 groups; control, diabetic, BMSCs treated and AMSCs treated. Treated groups were intravenously given 1 ml PKH26 labeled allogenic BMSCs or AMSCs suspended in phosphate buffered saline, respectively. Animals of all groups were sacrificed 2 weeks after stem cells administration. Sections from control and treated groups were examined by fluorescence microscope. Sections from all groups were immunohistochemically stained to detect insulin and proliferating cell nuclear antigen (PCNA). Mean area percent of insulin and number of PCNA positive reactions were measured and statistically analyzed. Results: Diabetic rats showed cell death and congested blood vessels in both exocrine and endocrine pancreas. Treated groups revealed homing of stem cells in pancreas after their transplantation. Moreover, nearly normal histological features were seen in AMSCs treated group. Studying the treated groups immunohistochemically, revealed increase in insulin and PCNA positive reactions when compared to diabetic group with more increase in AMSCs treated group than BMSCs treated group. Conclusion: Intravenous AMSCs could be more effective than BMSCs in treatment of STZ-induced type I diabetes.

Comparative study on the therapeutic effects of bone marrow mesenchymal stem cells versus platelet rich plasma on the pancreas of adult male albino rats with streptozotocin-induced type 1 diabetes mellitus

“…These changes are close to the observations of the congested blood vessels and darkly stained pyknotic nuclei of some diabetic rat acinar cells [46,42] that observed nearly all acinar cells in diabetic rats were swollen with small vacuoles. These results are consistent with Nurdiana et al [42] that revealed histological destruction of the pancreatic islets of diabetic rats.…”

Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate the Pancreatic Changes of Chemically Induced Hypothyroidism by Carbimazole in Male Rats

“…Additionally, BMMSCs injection has been demonstrated to successfully reconstruct the kidney injury associated with severe acute pancreatitis [Chen et al, 2013], treat hypertensive nephropathy [Mohamed and Samak, 2017], and improve rat pancreatic alterations after i.p. injection of streptozotocin [Omar and Aboulkhair, 2017]. Furthermore, BM-MSCs regenerated the parotid gland after ovariectomy in rats [Abd El-Haleem et al, 2018].…”

“…Their ability to regenerate damaged pancreas has not been previously studied. However, transplantation of rat's BM-MSCs to pancreatic islets could differentiate into functionally competent pancreatic β-cells [Omar and Aboulkhair, 2017]. In addition, these cells have been demonstrated to regenerate the thyroid gland in mice exposed to X-rays [Mikhailov et al, 2012].…”

Protective effects of either C‐peptide or l‐arginine on pancreatic β‐cell function, proliferation, and oxidative stress in streptozotocin‐induced diabetic rats

“…PA1‐36022). The reaction is cytoplasmic (Omar & Aboulkhair, ). PCNA antibody: It is a rabbit polyclonal antibody (Lab Vision Corporation Laboratories; cat.…”