Comparative study on the therapeutic effects of bone marrow mesenchymal stem cells versus platelet rich plasma on the pancreas of adult male albino rats with streptozotocin-induced type 1 diabetes mellitus

El-Haroun, Hala; Salama, Rasha

doi:10.5603/fm.a2021.0008

Cited by 4 publications

(5 citation statements)

References 55 publications

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“…GIIb showed a significant increase in Mean of the diameter of the islet when compared with GIIa. These results were consistent with (18,29,30) and there was a significant decrease in FBG level, area % of caspase 3, and collagen fibers as compared with GIIa this agreed by (5,31,32) who explained that pancreatic fibrosis may be caused by pancreatic stellate cells that have been activated by hyperglycemia, resulting in fibrotic changes. Group IIc had a significant increase in the mean diameter of islets and a significant decrease change in FBG level, collagen fibers, and caspase 3 immune reactivity as compared with GIIa and GIIb.…”

Section: Discussionsupporting

confidence: 85%

Effects of Insulin and Resveratrol Versus Mesenchymal Stem Cells (MSCs) on the pancreas of Diabetic Rats (Type-I) (Light and Electron Microscope Study)

Manawy,

EID,

Ali

et al. 2024

Benha Medical Journal

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Background: transplantation of mesenchymal stem cells (MSCs) provides an effective strategy to protect pancreatic tissue injury in diabetes type 1. Many factors act as obstacles to their best regenerative capacity. Aim of the work: To evaluate the role of Adipose-derived mesenchymal stem cell (AD-MSCs) in the treatment of type 1 DM and to compare their efficacy alone and with a combination with resveratrol and long-acting insulin in the pancreas of albino rats with streptozotocin induced T1DM. Methods: 10 out of the 50 healthy adult male albino rats served as donors for stem cells and the other 40 rats were subdivided into two main groups: Group I (control group (16 rats), Group II (STZ-diabetic group, (24) rats which were injected with a single intraperitoneal injection of streptozotocin 50 mg/kg BW. This group was subdivided into 4 groups. GII a: untreated rats, GII b: received 1x10⁶ of cultured rat ADMSCs once intravenously into the tail vein, GII c which was given MSCs plus resveratrol in combination, GII d which was given MSCs & resveratrol and insulin glargine. Pancreatic samples were prepared for immune histochemical study, light and electron microscopic examination, and morphometric studies. Results: The AD-MSCs-treated group revealed moderate improvement in histological structure and fasting blood glucose level. AD-MSCs, resveratrol and insulintreated group demonstrated higher improvement than that of the MSCs treated group alone or dual combination of AD-MSCs plus resveratrol. Conclusion: Better synergistic protective effect on damaged pancreatic tissues can be observed in STZ induced DM rats treated with AD-MSC coupling with resveratrol and insulin treatment.

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Section: Discussionsupporting

confidence: 85%

Effects of Insulin and Resveratrol Versus Mesenchymal Stem Cells (MSCs) on the pancreas of Diabetic Rats (Type-I) (Light and Electron Microscope Study)

Manawy,

EID,

Ali

et al. 2024

Benha Medical Journal

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“…This was also observed in the free control though, and can therefore not be attributed to the bioprinting but is more likely an artefact of batch variation. While other research has indicated that especially PRP can lead to the increased proliferation of pancreatic (β-)cells in vivo [ 40 , 62 ], we detected a positive influence of FFP-supplementation at early time points but not of PL-supplementation, despite mostly similar compositions. On the other hand, during in vivo studies, PRP was injected twice weekly and proliferation was seen in adult rats [ 40 , 59 ], whereas supplements were released from bioinks over time and not replenished.…”

Section: Discussioncontrasting

confidence: 78%

“…For rat islets in vitro, this resulted in an increased viability, as well as an increased insulin content and release with no reported SI [ 59 ]. In vivo, when diabetes was induced in rats, the injection of PRP led to an increase in the number of β-cells, increased insulin secretion, improved blood-glucose control, and reduced oxidative stress [ 40 , 41 , 59 , 62 ]. A positive effect on the number of β-cells was also observed when PRP was added during the differentiation of induced pluripotent stem cells [ 63 ].…”

Section: Discussionmentioning

confidence: 99%

Viability and Functionality of Neonatal Porcine Islet-like Cell Clusters Bioprinted in Alginate-Based Bioinks

et al. 2022

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The transplantation of pancreatic islets can prevent severe long-term complications in diabetes mellitus type 1 patients. With respect to a shortage of donor organs, the transplantation of xenogeneic islets is highly attractive. To avoid rejection, islets can be encapsulated in immuno-protective hydrogel-macrocapsules, whereby 3D bioprinted structures with macropores allow for a high surface-to-volume ratio and reduced diffusion distances. In the present study, we applied 3D bioprinting to encapsulate the potentially clinically applicable neonatal porcine islet-like cell clusters (NICC) in alginate-methylcellulose. The material was additionally supplemented with bovine serum albumin or the human blood plasma derivatives platelet lysate and fresh frozen plasma. NICC were analysed for viability, proliferation, the presence of hormones, and the release of insulin in reaction to glucose stimulation. Bioprinted NICC are homogeneously distributed, remain morphologically intact, and show a comparable viability and proliferation to control NICC. The number of insulin-positive cells is comparable between the groups and over time. The amount of insulin release increases over time and is released in response to glucose stimulation over 4 weeks. In summary, we show the successful bioprinting of NICC and could demonstrate functionality over the long-term in vitro. Supplementation resulted in a trend for higher viability, but no additional benefit on functionality was observed.

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“…Decrease in blood glucose levels and lesions in renal tissue [ 94 ] 1 UC-MSC Decrease in blood globulin and platelet level. Decrease in erythrocyte sedimentation rate, C-reactive protein level, and rheumatoid factor [ 95 ] 2 UC-MSC Decrease in Desease Activity Score-28. Decrease in IL-6 and increase in IL-10.…”

Section: Introductionmentioning

confidence: 99%

“…BM-MSCs were shown to be a better treatment than platelet-rich plasma injection in T1D rat model. The results showed that the group treated with BM-MSCs had an increase in the diameter of Langerhans islets and the amount of zymogen granules compared to the untreated group and the group treated with platelet-rich plasma [ 95 ]. A study tested the immunomodulatory capacity and beta cell protection of Ad-MSCs in a diabetic mice model.…”

Section: Introductionmentioning

confidence: 99%

Clinical utility of mesenchymal stem/stromal cells in regenerative medicine and cellular therapy

et al. 2023

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Mesenchymal stem/stromal cells (MSCs) have been carefully examined to have tremendous potential in regenerative medicine. With their immunomodulatory and regenerative properties, MSCs have numerous applications within the clinical sector. MSCs have the properties of multilineage differentiation, paracrine signaling, and can be isolated from various tissues, which makes them a key candidate for applications in numerous organ systems. To accentuate the importance of MSC therapy for a range of clinical indications, this review highlights MSC-specific studies on the musculoskeletal, nervous, cardiovascular, and immune systems where most trials are reported. Furthermore, an updated list of the different types of MSCs used in clinical trials, as well as the key characteristics of each type of MSCs are included. Many of the studies mentioned revolve around the properties of MSC, such as exosome usage and MSC co-cultures with other cell types. It is worth noting that MSC clinical usage is not limited to these four systems, and MSCs continue to be tested to repair, regenerate, or modulate other diseased or injured organ systems. This review provides an updated compilation of MSCs in clinical trials that paves the way for improvement in the field of MSC therapy.

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Comparative study on the therapeutic effects of bone marrow mesenchymal stem cells versus platelet rich plasma on the pancreas of adult male albino rats with streptozotocin-induced type 1 diabetes mellitus

Abstract: This article has been peer reviewed and published immediately upon acceptance. It is an open access article, which means that it can be downloaded, printed, and distributed freely, provided the work is properly cited. Articles in "Folia Morphologica" are listed in PubMed.

Cited by 4 publications

References 55 publications

Effects of Insulin and Resveratrol Versus Mesenchymal Stem Cells (MSCs) on the pancreas of Diabetic Rats (Type-I) (Light and Electron Microscope Study)

Effects of Insulin and Resveratrol Versus Mesenchymal Stem Cells (MSCs) on the pancreas of Diabetic Rats (Type-I) (Light and Electron Microscope Study)

Viability and Functionality of Neonatal Porcine Islet-like Cell Clusters Bioprinted in Alginate-Based Bioinks

Clinical utility of mesenchymal stem/stromal cells in regenerative medicine and cellular therapy

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