Effect of bone marrow stromal cells in combination with biomaterials in early phases of distraction osteogenesis: An experimental study in a rabbit femur model

Montes-Medina, Laura; Hernández-Fernández, Alberto; Gutiérrez-Rivera, Araika; Ripalda‐Cemboráin, Purificación; Bitarte, Nerea; Pérez-López, Virginia; Granero‐Moltó, Froilán; Prósper, Felipe; Izeta, Ander

doi:10.1016/j.injury.2018.09.007

Cited by 9 publications

(7 citation statements)

References 50 publications

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“…enhancing new bone formation during DO. A previous study showed that there were variations in the osteogenic differentiation potential of bone marrow-derived MSCs obtained from rabbits, leading to various bone healing outcomes in the rabbit DO model [38]. In this study, the osteogenic differentiation potential of bone marrow-derived MSCs isolated from rats proved to be consistent and was further verified by in vivo outcomes in the rat DO model.…”

Section: Discussionsupporting

confidence: 74%

Local administration of allogeneic or autologous bone marrow-derived mesenchymal stromal cells enhances bone formation similarly in distraction osteogenesis

Pan

et al. 2021

Cytotherapy

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Background aims: Distraction osteogenesis (DO) is a surgical technique to promote bone regeneration that requires a long time for bone healing. Bone marrow-derived mesenchymal stromal cells (MSCs) have been applied to accelerate bone formation in DO. Allogeneic MSCs are attractive, as they could be ready to use in clinics. Whether allogeneic MSCs would have an effect similar to autologous MSCs with regard to promoting bone formation in DO is still unknown. This study compares the effect of autologous MSCs versus allogeneic MSCs on bone formation in a rat DO model. Methods: Rat bone marrow-derived MSCs were isolated, characterized and expanded in vitro. Adult rats were subjected to right tibia transverse osteotomy. On the third day of distraction, each rat received one injection of phosphate-buffered saline (PBS), autologous MSCs or allogeneic MSCs at the distraction site. Tibiae were harvested after 28 days of consolidation for micro-computed tomography examination, mechanical test and histological analysis. Results: Results showed that treatment with both allogeneic and autologous MSCs promoted bone formation, with significantly higher bone mass, mechanical properties and mineral apposition rate as well as expression of angiogenic and bone formation markers at the regeneration sites compared with the PBS-treated group. No statistical difference in bone formation was found between the allogeneic and autologous MSC treatment groups. Conclusions: This study indicates that allogeneic and autologous MSCs have a similar effect on promoting bone consolidation in DO. MSCs from an allogeneic source could be used off-the-shelf with DO to achieve early bone healing.

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Section: Discussionsupporting

confidence: 74%

Local administration of allogeneic or autologous bone marrow-derived mesenchymal stromal cells enhances bone formation similarly in distraction osteogenesis

Pan

et al. 2021

Cytotherapy

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“…Preclinical nonunion rat model, subperiosteal osteotomy and critical size femoral defect rabbit models have been used to show that this is also an efficacious approach to repair skeletal tissue. While pre-differentiating BMSC towards osteogenic lineage in combination with biomaterials is one approach to facilitate skeletal repair clinically [ 137 , 138 , 139 ], the maturation of BMSC in vitro diminishes their capacity to survive when transplanted in vivo compared to immature BMSC. The paracrine effects of BMSC are also critical in expediting neovascularization within the scaffold [ 139 ].…”

Section: Bmsc Treatment For Bone Related Skeletal Diseases/disordementioning

confidence: 99%

Clinical Application of Bone Marrow Mesenchymal Stem/Stromal Cells to Repair Skeletal Tissue

Arthur

Gronthos

2020

IJMS

140

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There has been an escalation in reports over the last decade examining the efficacy of bone marrow derived mesenchymal stem/stromal cells (BMSC) in bone tissue engineering and regenerative medicine-based applications. The multipotent differentiation potential, myelosupportive capacity, anti-inflammatory and immune-modulatory properties of BMSC underpins their versatile nature as therapeutic agents. This review addresses the current limitations and challenges of exogenous autologous and allogeneic BMSC based regenerative skeletal therapies in combination with bioactive molecules, cellular derivatives, genetic manipulation, biocompatible hydrogels, solid and composite scaffolds. The review highlights the current approaches and recent developments in utilizing endogenous BMSC activation or exogenous BMSC for the repair of long bone and vertebrae fractures due to osteoporosis or trauma. Current advances employing BMSC based therapies for bone regeneration of craniofacial defects is also discussed. Moreover, this review discusses the latest developments utilizing BMSC therapies in the preclinical and clinical settings, including the treatment of bone related diseases such as Osteogenesis Imperfecta.

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“…Long bone reconstruction uses a distraction osteogenesis model—a surgical model that creates a gap in a long bone (usually tibia), using continuous traction on the osteotomy ends. This model was used with various rodent species (e.g., mouse [184,185,186,187], rat [188,189,190,191], and rabbit [192,193,194]) and even larger mammalians (e.g., sheep [195,196] or goats [197]). Endo-osseous implants are used for dental reconstruction or oromaxilofacial reconstruction after tumor resection [198], but their biocompatibility also needs testing [199].…”

Section: Efficacy Evaluation Of Cap-based Bioceramicsmentioning

confidence: 99%

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

et al. 2019

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Recently, a large spectrum of biomaterials emerged, with emphasis on various pure, blended, or doped calcium phosphates (CaPs). Although basic cytocompatibility testing protocols are referred by International Organization for Standardization (ISO) 10993 (parts 1–22), rigorous in vitro testing using cutting-edge technologies should be carried out in order to fully understand the behavior of various biomaterials (whether in bulk or low-dimensional object form) and to better gauge their outcome when implanted. In this review, current molecular techniques are assessed for the in-depth characterization of angiogenic potential, osteogenic capability, and the modulation of oxidative stress and inflammation properties of CaPs and their cation- and/or anion-substituted derivatives. Using such techniques, mechanisms of action of these compounds can be deciphered, highlighting the signaling pathway activation, cross-talk, and modulation by microRNA expression, which in turn can safely pave the road toward a better filtering of the truly functional, application-ready innovative therapeutic bioceramic-based solutions.

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Effect of bone marrow stromal cells in combination with biomaterials in early phases of distraction osteogenesis: An experimental study in a rabbit femur model

Cited by 9 publications

References 50 publications

Local administration of allogeneic or autologous bone marrow-derived mesenchymal stromal cells enhances bone formation similarly in distraction osteogenesis

Local administration of allogeneic or autologous bone marrow-derived mesenchymal stromal cells enhances bone formation similarly in distraction osteogenesis

Clinical Application of Bone Marrow Mesenchymal Stem/Stromal Cells to Repair Skeletal Tissue

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

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