Effect of bone marrow mesenchymal stem cells transplantation on BMP-15 expression and Graafian follicle count in mice model of endometriosis

Parasti, Ratriana Via; Widjiati, Widjiati; Dwiningsih, Sri Ratna

doi:10.20473/mog.v26i32018.107-111

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…Then, the preparations were cleaned and dropped with mounting media and covered with a coverslip. Follicular development was observed on the cells using a light microscope with 400×, which was previously confirmed at 1000× [ 25 , 26 ].…”

Section: Methodsmentioning

confidence: 60%

Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models

et al. 2021

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Background and Aim: Endometriosis affects the ovaries and causes a decrease in the oocyte quality during endometrial receptivity. During the development of ovarian follicles, paracrine communication occurs between granulosa cells and oocytes. This study was conducted to determine the effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha (TNF-α) receptor 1 (TNFR1) expression, granulosa cell apoptosis, and folliculogenesis in endometriosis mouse models. Materials and Methods: This study involved 42 female mice, which were divided into three groups: Healthy mice (T0), endometriosis mice without transplantation (T1), and endometriosis mice with bone marrow mesenchymal stem cell transplantation (T2). The mice were injected intraperitoneally with endometrial fragments (200 μL) to become endometriosis models. On day 15, the endometriosis models received mesenchymal stem cells. Sample collection was performed on day 29. Granulosa cell apoptosis and TNFR1 expression were examined using immunohistochemical staining, and folliculogenesis was assessed using hematoxylin and eosin staining of ovary samples. The data obtained from both examinations were statistically analyzed using Statistical Package for the Social Sciences. Results: The results showed that TNFR1 expression is significantly decreased in T2 (p<0.004). The apoptosis of granulosa cells was lower in T2 (p<0.000). The primary, secondary, and graafian follicle counts in T2 were significantly increased. Conclusion: Bone marrow mesenchymal stem cell transplantation in endometriosis mouse models can reduce TNFR1 expression and granulosa cell apoptosis and improve folliculogenesis.

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Section: Methodsmentioning

confidence: 60%

Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models

et al. 2021

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“…Data for each sample was evaluated semi quantitatively using the modified Remmele method. 37,42,43 The examination of DeGraff follicle count…”

Section: The Examination Of Tnf-α Expression Using Immunohistochemica...mentioning

confidence: 99%

The Effect of Syzygium Polyanthum (Wight) Walp. Extract on Glutathione, Tumour Necrosis Factor-Alpha, Spdl1, And Degraff Follicles Expression in PCOS Rat Models

Aditya,

Santoso,

Widjiati W

2023

Pharmacogn J.

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Background: Polycystic ovary syndrome (PCOS) is an endocrine and reproductive disorder affecting 5% to 13% of women of reproductive age. Approximately 80% of women with PCOS underwent infertility cases. Many studies have shown an association between PCOS and low-grade inflammation. Syzygium polyanthum (S. polyanthum) contains antioxidants and has antiinflammation activity. Objectives: This study aims to investigate the effectiveness of S. polyanthum leaf on glutathione, tumour necrosis factoralpha (TNF-α), sPDL1, and DeGraff follicle expression in PCOS rat models. Materials and Methods: The female Wistar rats were divided into five groups (n = 5), K0 (normal control), K1 (PCOS group), and three treatment groups which received three different doses of S. polyanthum extract. The treatment group consisted of PCOS rat models with S. polyanthum extract supplementation of 150 mg/KgBW (P1), 300 mg/KgBW (P2), and 450 mg/KgBW (P3). Results: S. polyanthum leaf extract significantly decreased TNF-α expression in the treatment group. The highest TNF-α, GSH, and sPDL1 concentration was found in the P3 group (3.83±0.123, 273.12±12.4 ng/ml, and 3.25 ng/ml, respectively). Moreover, the number of follicles was statistically significant in the P1, P2, and P3 groups (2.0, 3.0, and 3.50, respectively). The number of follicles in the P1, P2, and P3 groups was higher than in the positive control; however, it was lower in the negative control (K0) (p<0.05). Conclusion: S. polyanthum extract was significantly effective in decreasing TNF-α expression, as well as a significant increase GSH, sPDL1 and DeGraff foliclle count in PCOS rat model.

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Effect of bone marrow mesenchymal stem cells transplantation on BMP-15 expression and Graafian follicle count in mice model of endometriosis

Cited by 2 publications

References 17 publications

Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models

Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models

The Effect of Syzygium Polyanthum (Wight) Walp. Extract on Glutathione, Tumour Necrosis Factor-Alpha, Spdl1, And Degraff Follicles Expression in PCOS Rat Models

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