Effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha receptor 1 expression, granulosa cell apoptosis, and folliculogenesis repair in endometriosis mouse models

Abstract

Background and Aim: Endometriosis affects the ovaries and causes a decrease in the oocyte quality during endometrial receptivity. During the development of ovarian follicles, paracrine communication occurs between granulosa cells and oocytes. This study was conducted to determine the effects of bone marrow mesenchymal stem cell transplantation on tumor necrosis factor-alpha (TNF-α) receptor 1 (TNFR1) expression, granulosa cell apoptosis, and folliculogenesis in endometriosis mouse models. Materials and Methods: This study involved 42 female mice, which were divided into three groups: Healthy mice (T0), endometriosis mice without transplantation (T1), and endometriosis mice with bone marrow mesenchymal stem cell transplantation (T2). The mice were injected intraperitoneally with endometrial fragments (200 μL) to become endometriosis models. On day 15, the endometriosis models received mesenchymal stem cells. Sample collection was performed on day 29. Granulosa cell apoptosis and TNFR1 expression were examined using immunohistochemical staining, and folliculogenesis was assessed using hematoxylin and eosin staining of ovary samples. The data obtained from both examinations were statistically analyzed using Statistical Package for the Social Sciences. Results: The results showed that TNFR1 expression is significantly decreased in T2 (p<0.004). The apoptosis of granulosa cells was lower in T2 (p<0.000). The primary, secondary, and graafian follicle counts in T2 were significantly increased. Conclusion: Bone marrow mesenchymal stem cell transplantation in endometriosis mouse models can reduce TNFR1 expression and granulosa cell apoptosis and improve folliculogenesis.

Therapeutic effects of L‐arginine, L‐carnitine, and mesenchymal stem cell‐conditioned medium on endometriosis‐induced oocyte poor quality in an experimental mouse model

“…The obtained results showed that BMSC‐CM therapy could mitigate nitro‐oxidative stress and consequently improved blastocyst development and quality in EMS mice. Our observation is in line with the findings of Dwiningsih et al's study, which indicated that BMSC exert protection on EMS by suppressing inflammation and apoptosis in granulosa cells and supporting folliculogenesis in EMS mouse models 21 . Additionally, we recently indicate that BMSC‐CM supplementation during maturation improved IVM, fertilization, and the subsequent development of EMS‐induced oocytes 12 .…”

“…Our observation is in line with the findings of Dwiningsih et al's study, which indicated that BMSC exert protection on EMS by suppressing inflammation and apoptosis in granulosa cells and supporting folliculogenesis in EMS mouse models. 21 Additionally, we recently indicate that BMSC-CM supplementation during maturation improved IVM, fertilization, and the subsequent development of EMS-induced oocytes. 12 Indeed, BMSC-CM provides a variety of pro-inflammatory cytokines, growth factors, and anti-apoptotic and antioxidant substances, and we were not able to determine the actual levels of these factors in plasma.…”

“…According to our results, induced EMS in a mouse model was associated with reduced oocyte quality and impaired oocyte and embryo development. The IVF results using oocyte donors have consistently shown that EMS affects the ovaries and degrades the quality of oocytes 21 . However, the exact mechanism by which EMS‐associated sub/infertility decreases oocyte quality remains obscure.…”

“…The IVF results using oocyte donors have consistently shown that EMS affects the ovaries and degrades the quality of oocytes. 21 However, the exact mechanism by which EMS-associated sub/infertility decreases oocyte quality remains obscure. In a normal condition, the level of reactive oxygen species (ROS) and antioxidants remains balanced.…”

Effect of Endometriosis on Cumulus ATP, Number of Mitochondria and Oocyte Maturity in Cumulus Oocyte Complex in Mice

“…To determine the sample size, the Lemeshow formula was used, with the result that each group consisted of 16 mice. 15 The making of endometriosis model mice was in accordance with what was done in the study by Hendarto. 16 In the present study, the presence of endometriosis was confirmed by the formation of endometriotic lesions and visually visible hypervascularization of the peritoneal tissue at surgery.…”