Effect of bone marrow mesenchymal stem cells on healing of temporomandibular joints in rats with induced rheumatoid arthritis

Abstract

The healing capacity of bone marrow mesenchymal stem cells (BMMSCs) has been evaluated in various studies. This study aimed to evaluate the effect of BMMSCs on the healing of temporomandibular joints (TMJs) with induced rheumatoid arthritis. Fifty healthy male Sprague Dawley rats were divided into three groups: group I (n = 10), negative control; group II (n = 20), positive control (induction of arthritis by adjuvant followed by intravenous injection of 0.1 ml of PBS); and group III (n = 20), intervention (as for group II but injected intravenously with 1 × 10 cells ml of BMMSCs suspended in PBS). Half of the rats in each group were euthanized 3 wk after the start of the experiment and the other half was euthanized after 5 wk. Group I revealed normal TMJ features. Group II showed thickening of disc, thinning of cartilage, disordered bone trabeculae, and decreased in mean % area staining positive of collagen fibers at 3 wk, while at 5 wk these effects were more aggravated. Group III showed nearly normal thickness of disc and condylar cartilage, nearly normal arrangement of bone trabeculae and regenerated collagen fibers at 3 wk, while after 5 wk the TMJ features were almost normal. Two-way anova revealed statistically significant differences between groups. Thus, treatment of induced rheumatoid arthritis with BMMSCs shows promising results that need to be further investigated in humans.

Transplantation of IL‑1β siRNA‑modified bone marrow mesenchymal stem cells ameliorates type II collagen‑induced rheumatoid arthritis in rats

“…BMSCs, as matrix stem cells, undergo chondrogenic differentiation and are capable of promoting cartilage repair and tissue regeneration (54). BMSCs can not only differentiate into multiple tissue cells, but also enhance the repair of tissue damage caused by inflammation by secreting various matrix molecules, which suggests that they may aid in regenerating RA cartilage (54)(55)(56). Mature articular cartilage has a limited capacity for self-regeneration after injury due to the lack of blood and nerve supply, resulting in poor articular cartilage recovery after damage (57).…”

Treatment of Mandibular Hypomobility by Injections into the Temporomandibular Joints: A Systematic Review of the Substances Used

“…These include, first of all, the blood products already discussed. Apart from them, there are the first experimental and clinical studies on intra-articular administration of adipose tissue and bone marrow cells [39,45,[93][94][95]. The promising results of these therapies do not exempt them from caution in their implementation [39,45].…”

MicroRNA-126 promotes proliferation, migration, invasion and endothelial differentiation while inhibits apoptosis and osteogenic differentiation of bone marrow-derived mesenchymal stem cells

“…And another study reports that the functions of immunomodulation and differentiation in BMSCs are inhibited in an environment of RA lymphocyte activation [36]. In addition, BMSCs also display treatment potential in RA, for example, a former study elucidates that BMSC injection ameliorates RA in rat models regarding the thickness of dis and condylar cartilage, bone trabeculae and regenerated collagen [37]. And a further clinical trial illustrates that using BMSC transplantation in patients with refractory RA decreases Th17 proportion, IL-17A level, DAS28-ESR and VAS score; however, the sample size in this trial is small, indicating that the results should be validated in a larger sample size [38].…”