Effect of Bone Marrow Mesenchymal Stem Cell Transplant on Synovial Proliferation in Rats With Type II Collagen-Induced Arthritis

Abstract

Objectives: To investigate the influence and mechanism of bone marrow mesenchymal stem cell transplant in the synovial proliferation of type II collagen-induced arthritis. Materials and Methods: From the bone marrow of Sprague-Dawley rats, mesenchymal stem cells were isolated and expanded. Forty rats were randomly divided into 5 groups: normal control, early mesenchymal stem cell treatment, late mesenchymal stem cell treatment, early collagen-induced arthritis control, and late collagen-induced arthritis control. The mesenchymal stem cells and normal saline were injected through the tail vein, and the following parameters were observed: arthritis index, articular pathology changes, serum vascular endothelial growth factor level, tumor necrosis factor-α, and interluekin-17 levels as detected through stable enzyme-linked immunosorbent assay. Results: The arthritis index and articular pathologic scores of the early and late treatment groups were lower compared with those of the control groups (P < .05). The arthritis index and articular pathologic scores of the late treatment group were lower than those of the early treatment group (P < .05). The levels of vascular endothelial growth factor, tumor necrosis factor-α, and interluekin-17 of the early and late treatment groups were significantly decreased compared with the collagen-induced arthritis control groups (P < .05), and these levels were positively correlated with the arthritis index and articular pathologic scores (P < .05). Conclusions: The transplant of mesenchymal stem cells in rats with collagen-induced arthritis can inhibit the proliferation of synovium, which may be attributed to the reduced expression of vascular endothelial growth factor, tumor necrosis factor-α, and interluekin-17.

Etanercept-Synthesising Mesenchymal Stem Cells Efficiently Ameliorate Collagen-Induced Arthritis

“…reported that MSCs do not have any effects in CIA mice31. However, various studies report beneficial effects of MSCs in CIA mice323334353637. The use of various cell strains and differences in the infused cell number or the administration route make it difficult to compare and interpret the inconsistent results of previous studies38.…”

Mesenchymal stem cells alleviate experimental rheumatoid arthritis through microRNA-regulated IκB expression

“…Several studies have reported the therapeutic effects of allogeneic or xenogenic MSC transplantation in CIA-mice 30 31 32 33 34 35 36 40 41 . Nevertheless, the underlying mechanisms are poorly understood.…”

“…Mesenchymal stem cells (MSCs) are multipotent progenitor cells that can differentiate into tissues of mesenchymal lineage, including bone, cartilage and adipose tissue 27 28 29 . Several studies have reported therapeutic effects of allogenic or xenogenic MSC transplantation in CIA mice 30 31 32 33 34 35 36 . However, the underlying molecular basis of these effects is not fully understood.…”

Mesenchymal stem/stromal cell-based therapy for the treatment of rheumatoid arthritis: An update on preclinical studies

“…Numerous studies have observed improved efficacy when the MSC infusion was performed during the early phases of the disease (47% and 25% in the onset and in the established phase of the disease, respectively) rather than at pre-onset (19%) or during chronic phases (9%) of the disease, although promising results in terms of efficacy were observed in all instances ( Fig. 5 a, Supplementary Tables 1 and 2) [ [33] , [34] , [35] , [71] , [72] , [73] , [74] ]. In contrast, the completed RA clinical trials with MSC-based therapy have been conducted in very refractory RA patients with a long history of the disease which may have hindered the beneficial effects of MSC-based therapy in the clinic.…”