Effect of Boerhaavia diffusa in experimental prostatic hyperplasia in rats

Abstract

Objective:Present investigation was undertaken to study the effectiveness of hydroalcoholic extract of roots of Boerhaavia diffusa in experimental benign prostatic hyperplasia (BPH) in rats using various animal models.Materials and Methods:BPH in rats was induced by subcutaneous injection of testosterone (5 mg/kg) daily for 28 days. Rats were divided in to five groups (six rats each). A negative control group received arachis oil (1 ml/kg s.c.) and four groups were injected testosterone. These four groups were further divided into reference group (finasteride 1 mg/kg), model group (testosterone), study group A (B. diffusa 100 mg/kg), and study group B (B. diffusa 250 mg/kg). On the 29th day, rats were sacrificed and body weight, prostate weight, bladder weight, and serum testosterone level were measured and histological studies were carried out. Further in vitro analysis of B. diffusa extract on contractility of isolated rat vas deferens and prostate gland, produced by exogenously administered agonists were carried out. All results were expressed as mean ± SEM. 0 Data were analyzed by one-way analysis of variance followed by Tukey's test.Results:B. diffusa (100 mg/kg) treatment for 28 days resulted in significant inhibition of prostate growth (P < 0.05). Drug extract did not have significant change on serum testosterone level. Histopathological analysis of prostate gland supported above results. Results of in vitro experiment suggest that extracts had attenuated the contractile responses of isolated vas deferens and prostate gland to exogenously applied agonists.Conclusion:The results suggested that treatment with B. diffusa may improve symptoms of disease and inhibit the increased prostate size. In vitro study implies that herbal extracts has the machinery to produce beneficial effect on prostatic smooth muscle, which would relieve the urinary symptoms of disease. B. diffusa could be a potential source of new treatment of prostatic hyperplasia.

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“…Many experimental studies showed that treatment with B. diffusa significantly decreases the prostate weight and prostatic index in rat. Prostatic index is one of the important markers of disease, which is calculated by prostate weight to body weight ratio 58 . In-vitro study implies that herbal extracts has valuable effect on prostatic smooth muscle, which would relieve the urinary symptoms of disease.…”

Pharmacological investigation of an Ayurvedic formulation on testosterone propionate-induced benign prostatic hyperplasia rats

“…Indian system of medicine is a rich collection of knowledge on traditional medicine which narrates many plants having anti-inflammatory, diuretic, antioxidant and 5α-reductase activity [10]. The herbo-mineral formulation used in this study is such an Ayurvedic proprietary which contains extracts of Crataeva nurvala (Varun) bark [11][12][13], Punarnavadi-Kwath formulation [14][15][16], Mimosa pudica (Lajjalu) Panchang [17], Curcuma longa (Haridra) rhizome [18], Emblica officinalis (Amalaki) fruit [19], Tinospora cordifolia (Guduchi) stem [20], Tribulus terrestris (Gokshur) fruit [21], powders of Chandraprabha Vati [21], and Shuddha Shilajit [21]. It has already been proven as standardized Ayurvedic herbo-mineral formulation [22].…”

Clinical evaluation of polyherbal formulation (Uricare Tablet) in benign prostatic hyperplasia: randomized, placebo controlled, single blinded clinical study

“…Polypeptide growth factors play an important role in disease development. 4 The complications of BPH include frequency of micturition, nocturia, dysuria and urgency or urge incontinence. Acute retention of urine is an unfortunate obstructive complication.…”

“…So, phytotherapy has gained momentum as an alternative for the management of BPH worldwide. 4,5 Hence, the prime objective of this study is evaluate the efficacy and safety of Uricare Tablet in BPH by determining the prostate volume, Level of Serum PSA and also by evaluating the IPSS score which can influence the quality of life. It is a polyherbal formulation indicated for BPH and each tablet of Uricare contains powders of Ext.…”

“…B. diffusa extract inhibit isolated vas deference contractility induced by noradrenaline suggest that it might have acted on α-receptor. 4 Commiphora mukul: It supports kidney and bladder health, detoxification of the urinary tract and also support against enlarged prostate, urinary tract disorders, urinary Stones, inconsistent urination. 9 All the raw materials are obtained from the various sources and quality controlled test are performed in the quality control department of the "Petlad mahal arogya mandal pharmacy" with the reference of the Ayurvedic Pharmacopoeia.…”