Effect of body weight in the pathogenesis of ligature-induced periodontal disease in Wistar rats

Simch, Ralf Priesnitz; Gaio, Eduardo José; Rösing, Cassiano Kuchenbecker

doi:10.1080/00016350802004672

Cited by 25 publications

(14 citation statements)

References 35 publications

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“…The animals were randomly assigned at baseline, by means of draw stratified by body weight, into two groups as follows: Obese (n=13), which received standard feeding (Supralab, Supra, São Leopoldo, RS, Brazil) and a complementary calorie-rich diet ["cafeteria diet" (CAF diet), consisting of chocolate cookies, sugar-rich milk and fat cheese] 5,19,22 ; Non-obese (n=11), which received standard feeding (Supralab, Supra, São Leopoldo, RS, Brazil).…”

Section: Methodsmentioning

confidence: 99%

“…Sample size calculation was performed using data from our previous study 19 . Taking into consideration a mean difference in alveolar bone loss of 0.2 mm, accepting alpha and beta errors of 0.05 and 0.20, respectively, a number of nine animals per group was considered necessary.…”

Section: Methodsmentioning

confidence: 99%

“…The contra-lateral maxillary molar was considered the internal control 10,19,24 . After 30 days of experimental periodontitis 19,24 , animals were killed using a carbon dioxide chamber (day 120). The experimental protocol was approved by Ethical Committee of the Lutheran University of Brazil (CEP-ULBRA 2004-027A).…”

Section: Methodsmentioning

confidence: 99%

“…Following sacrifice, the left and right segments of the maxillae were dissected out manually and then immersed in sodium hypochlorite with 8.5% active chlorine (Mazzarollo, Gravataí, RS, Brazil) during 5 h to remove soft tissues 10,19,24 . After rinsing, the specimens were stained for 1 min in methylene blue 1% (Sigma-Aldrich, Saint Louis, MO, USA) to delineate the cementoenamel junction.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, our research group published a study assessing the progression of alveolar bone loss in rats with overweight and normal weight, using a ligature-induced periodontal disease model in Wistar rats 19 . The results demonstrated that overweight rats did not present higher alveolar bone loss as compared to controls.…”

Section: Introductionmentioning

confidence: 99%

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Effect of obesity on alveolar bone loss in experimental periodontitis in Wistar rats

et al. 2012

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Obesity has been linked to higher inflammatory status and periodontal breakdown.Objective The purpose of this study was to investigate the effect of obesity on alveolar bone loss in experimental periodontitis in rats. Material and Methods Twenty-four female Wistar rats were randomly divided into two groups: obese (n=13), which were fed with "cafeteria diet" (CAF diet - high amounts of sucrose and fat) for 90 days in order to gain weight, and non-obese (n=11) regularly fed rats. Ligature-induced experimental periodontitis was created in all animals. Body weight differed statistically between obese and non-obese groups (277.59 and 223.35 g, respectively) at the moment of the ligature placement. Morphometric registration of alveolar bone loss was carried out after 30 days of ligature placement to determine the effect of obesity on the progression of experimental periodontitis. Results Intra-group comparisons showed significantly higher alveolar bone loss mean values in maxillary teeth with ligature (P<0.05). Alveolar bone loss [mean (SD), mm] was not statistically different between obese and non-obese groups [0.71 (0.09) and 0.65 (0.07) mm, respectively]. However, when palatal sides are analyzed separately, obese group presented significantly higher alveolar bone loss (P<0.05) as compared to non-obese [0.68 (0.12) and 0.53 (0.13) mm, respectively]. Conclusions In spite of the weak differences, it is possible to conclude that the progression of alveolar bone loss in ligature-induced periodontitis can be potentially influenced by body weight in rats.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of obesity on alveolar bone loss in experimental periodontitis in Wistar rats

et al. 2012

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Evaluation of recurrence of periodontal disease after treatment in obese and normal weight patients: Two‐year follow‐up

Zuza

Pires

Almeida

et al. 2020

Journal of Periodontology

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Background Obesity may represent a chronic low‐grade inflammation, but there is a lack of long‐term longitudinal studies. The aim of this longitudinal study was to evaluate the recurrence of periodontal disease in obese and normal weight patients submitted to scaling and root planing. Methods The study included 22 patients who had received periodontal treatment 2 years previously, 13 obese and nine non‐obese. The patients were evaluated for anthropometric measurements of body mass index, waist circumference, waist‐to‐hip ratio, and fat percentage through bioimpedance. The following periodontal parameters were recorded: visible plaque index (VPI), gingival bleeding index (GBI), probing depth (PD), clinical attachment level (CAL), and bleeding on probing (BOP). The immunological evaluation analyzed the proinflammatory cytokines interleukin‐1 beta (IL‐1β), interleukin‐6 (IL‐6), and tumor necrosis factor‐alpha (TNF‐α) in the gingival crevicular fluid (GCF). Results Obese and normal weight patients did not differ in relation to the periodontal parameters of VPI, GBI, PD, CAL, or BOP 2 years after completion of the periodontal therapy. Sites with periodontitis in obese individuals showed higher levels of IL‐6 and TNF‐α in the gingival fluid (P <0.05). Conclusion Obese and normal weight individuals had similar periodontal behaviors, with low recurrence of the periodontal disease; however, obesity was related to increased inflammatory activity in gingival fluid, which may become a risk indicator for future greater recurrence of the disease in the presence of inadequate plaque control.

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Influence of obesity on experimental periodontitis in rats: histopathological, histometric and immunohistochemical study

et al. 2017

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Effect of body weight in the pathogenesis of ligature-induced periodontal disease in Wistar rats

Abstract: The establishment and progression of alveolar bone loss in rats was not influenced by body weight in the present study.

Cited by 25 publications

References 35 publications

Effect of obesity on alveolar bone loss in experimental periodontitis in Wistar rats

Effect of obesity on alveolar bone loss in experimental periodontitis in Wistar rats

Evaluation of recurrence of periodontal disease after treatment in obese and normal weight patients: Two‐year follow‐up

Influence of obesity on experimental periodontitis in rats: histopathological, histometric and immunohistochemical study

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