Effect of Body Vibration on Structural Organisation of Tibia Nanocomposites in Rats With Glucocorticoid-Induced Osteoporosis

Abstract

The aim of the study was to evaluate the effect of nonphysiological whole body vibration (0,3g) on the bone structure and metabolism in rats treated with methylprednisolone (3 mg/ kg/day every other day, 24 weeks). Amount of crystalline component and collagen in the bones was determined by X-ray diffraction method, and the level of calcium by atomic adsorption spectroscopy. Bone metabolism was assessed by determining the concentration of markers - osteocalcinandtartrate-resistant acid phosphatase 5b. Methylprednisolone reduced the content of the mineral component in the tibia (–16.8%) in I group compared with the control. This significantly accelerated the process of bone metabolism, as evidenced by the increased level of bone remodeling markers. It should be noted that the total nonphysiological whole body vibration did not allow a decrease in the mineral component of the bone until 16 weeks of the experiment compared with I group, although these values were lower than the control group (–28.3%). We suggests that mechanical high-frequency low-intensity whole body vibration can inhibit the negative effects of glucocorticoids on bone structure.