Effect of bixin on DNA damage and cell death induced by doxorubicin in HL60 cell line

Abstract

<jats:p> Bixin is a natural red pigment extracted from annatto. Although it is widely used as a coloring agent in food, there are few studies about the effect of this carotenoid on DNA. This study aimed to investigate the effects of bixin on cytotoxicity and genotoxicity induced by doxorubicin in HL60 cells. At concentrations above 0.3 μg/mL, bixin demonstrated cytotoxic effects in HL60 cells. Furthermore, this carotenoid was neither mutagenic nor genotoxic to HL60 cells and reduced the DNA damage induced by doxorubicin. Bixin and doxorubicin showed no apoptotic effect in HL60 cells, but the simultaneous combined treatments showed an increase in the percentage of apoptotic cells. In conclusion, our results showed that bixin modulates the cytotoxicity of doxorubicin via induction of apoptosis. The results of this study provide more knowledge about the toxic effects of anticancer treatments and how the natural compounds can be useful on these therapeutic approaches. </jats:p>

Bixin, an apocarotenoid isolated from Bixa orellana L., sensitizes human melanoma cells to dacarbazine-induced apoptosis through ROS-mediated cytotoxicity

“…As previously reported, bixin has anti and pro-oxidant potential based on the tested concentrations and other biochemical factors (Anantharaman et al, 2016;Mohan et al, 2018;Tay-agbozo et al, 2018). The pharmacological activities of bixin have been demonstrated mostly on carcinogenesis and lipid peroxidation (Anantharaman et al, 2016;Santos et al, 2016), but the involvement of its pro-oxidant effect in sensitizing tumor cells to chemotherapy has not been properly explored so far.…”

“…(prostate), HCT-116 (colon), MCF-7 (breast), DRO (anaplastic thyroid) and (papillary thyroid) cell lines. Bixin inhibited the proliferation of tumor cells in a concentration-dependent manner, through apoptosis induction and cell cycle arrest (Anantharaman et al, 2016;Santos et al, 2016;Tibodeau et al, 2010). Interestingly, this metabolite has shown low toxicity in animal models (Agner et al, 2004;Bautista et al, 2004;Paumgartten et al, 2002;Stohs, 2014), and it is therefore considered as a good candidate for sensitizing tumor cells.…”

Synthesis, Leishmanicidal, Trypanocidal, Antiproliferative Assay and Apoptotic Induction of (2-Phenoxypyridin-3-yl)naphthalene-1(2H)-one Derivatives

“…Early apoptosis was defined by annexin V-FITC expression and, necrosis by PI expression, late apoptosis by coexpression of annexin V/PI. Doxorubicin and quercetin were used as positive controls [ 51 , 52 , 53 , 54 , 55 , 56 ]. The results of a typical flow cytometry analysis of both compounds are illustrated in Figure 2 and Figure 3 .…”

Synthesis and antimalarial and anticancer evaluation of 7‐chlorquinoline‐4‐thiazoleacetic derivatives containing aryl hydrazide moieties

“…Doxorubicin and CQs were used as the reference drugs. [ 52–57 ] Figure 1 represents a typical flow cytometry assessment using both cell lines. The maximum effect of doxorubicin and CQ was recorded at 1 and 100 µM, respectively.…”