“…As well as preliminary tests for materials that are utilized for root-end filling, furcal perforation, and as apical barriers, such materials must be investigated for their biocompatibility characteristics, as toxic components present in them might induce irritation or even degeneration of the surrounding tissues, especially is they are accidentally extruded into the periradicular tissues (4,25). Portland cement and MTA have similar major constituents, except for bismuth oxide, which is added to MTA to provide radiopacity (11,26,27). Although this feature does not compromise the execution of pulpotomy procedures with PC and their follow-up assessments, this property is necessary in order to distinguish the material from surrounding anatomical structures such as tooth and bone (11,17,18,28), and to permit the material to achieve the minimum values recommended by the American National Standards Institute / American Dental Association (ANSI/ADA) standard No 57/2000 (29).…”