Effect of Biphasic Calcium Phosphate Treated with Vascular Endothelial Growth Factor on Osteogenesis and Angiogenesis Gene Expression In Vitro

Hamid, Hamdan; Ahmad, Azlina; Khamis, Mohd Fadhli; Rahman, Roselinda Abd; Razak, Noor Hayati Abdul; Mutum, Samarendra Singh; Samsudin, Abdul Rani

doi:10.1007/978-3-319-02913-9_61

Cited by 3 publications

(4 citation statements)

References 17 publications

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“…The goal of developing novel approach strategies for bone replacement with bone substitutes are focused on stimulation of osteointegration, osteoconduction, osteoinduction as well as induction of angiogenesis and vascularisation 16) . Dental sources MSCs can be applied to obtain suitable autologous DSCs for tissue replacement therapies of both bone and cartilage 17) .…”

Section: Discussionmentioning

confidence: 99%

Enhanced Osteogenic and Angiogenic-Related Gene Expression of Human Dental Stem Cells on Biphasic Calcium Phosphate Scaffold Treated with Vascular Endothelial Growth Factor: Part I

Enezei

Ahmad

Khamis

et al. 2017

J. Hard Tissue Biology.

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To study the eff ectiveness of vascular endothelial growth factor (VEGF) added with biphasic calcium phosphate (BCP) on the expression of osteogenesis and angiogenesis-related gene in dental stem cells (DSCs). The cells were treated with three diff erent modalities; BCP group, VEGF group, and VEGF-added-BCP. The optimal BCP and VEGF concentrations were determined. The cells were harvested at four diff erent time intervals (day 3, day 7, day 10 and day 14) and were subjected to RNA isolation. Osteogenesis and angiogenesis-regulated genes was amplifi ed using reverse transcriptase-PCR (RT-PCR). The RT-PCR products were then electrophoresed. The gel images were captured using Image Analyser AlphaEaseFC™. Angiogenesis and osteogenesis genes were clearly expressed in DSCs in response to treatments with 75mg/ml BCP and 5ng/ml VEGF. Angiogenesis gene VEGF was highly expressed by VEGF treatment group but showed some changes when added with BCP. Osteogenesis genes (BMP-2 and OPN) were positively aff ected by both BCP and VEGF. Some genes were expressed at an earlier time interval compared to the other genes depending on the type of treatments. BCP treatment induced high expression of initial-regulated osteogenesis genes (BMP-2 and OPN). Combination of BCP and VEGF modality on DSCs was suggested to initiate osteogenesis and angiogenesis-related gene expressions earlier than the other modalities. In our design, uncontrolled release of VEGF protein showed inhibition of BMP-2 at mRNA level in VEGF-BCP combined group.

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Section: Discussionmentioning

confidence: 99%

Enhanced Osteogenic and Angiogenic-Related Gene Expression of Human Dental Stem Cells on Biphasic Calcium Phosphate Scaffold Treated with Vascular Endothelial Growth Factor: Part I

Enezei

Ahmad

Khamis

et al. 2017

J. Hard Tissue Biology.

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“…Efficiency Skill based Learning [16] Inability to provide hands-on practice Attentiveness [17] No Case based Learning…”

Section: Sub-domain Limitationsmentioning

confidence: 99%

“…Learning Environment [16] Connectivity, multi-learning, conceptualized learning, lifelong learning, learning with integrity…”

Section: Sub-domain Limitationsmentioning

confidence: 99%

“…Technological Perspective [18] Service-driven architecture, learning process enhancement, open access educational platform, speed, high-quality immersive learning environment Availability [16] Unprecedented ubiquitous access to data, learning encouragement, localized learning, participative learning spaces, session recording advantages…”

Section: Sub-domain Limitationsmentioning

confidence: 99%

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Challenges and Opportunities for Online Learning in India

Shanthini¹

2022

RRRJ

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Today's digital world demands more effective learning models that allow students to actively participate/enrol in their education. Digital technology is influencing the way the instruction is delivered as well as the way the information is discovered and shared. Recently, the adoption of internet and mobile learning technologies have changed the traditional perspective of education. In India, e-learning could be the viable solution to provide education in the context of 21st century. Effective mobile and e-learning applications can be developed by conducting an analysis on the use of mobile devices and applications in online education. Despite numerous initiatives to promote the use of mobile learning for education, there are some issues that must be addressed. By considering the sudden shift towards online teaching in the rise of COVID-19 pandemic in India, this study summarizes the research findings of mobile and e-learning technologies to avoid potential complications with the implementation of this technology during any future pandemic situations in India. The proposed study also assists in understanding the current state of mobile learning technology adoption in India.

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Calcium Phosphates: A Key to Next‐Generation In Vitro Bone Modeling

Pandit,

Indurkar,

Locs

et al. 2024

Adv Healthcare Materials

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The replication of bone physiology under laboratory conditions is a prime target behind the development of in vitro bone models. The model should be robust enough to elicit an unbiased response when stimulated experimentally, giving reproducible outcomes. In vitro bone tissue generation majorly requires the availability of cellular components, the presence of factors promoting cellular proliferation and differentiation, efficient nutrient supply, and a supporting matrix for the cells to anchor – gaining predefined topology. Calcium phosphates (CaP) are difficult to ignore while considering the above requirements of a bone model. Therefore, the current review focuses on the role of CaP in developing an in vitro bone model addressing the prerequisites of bone tissue generation. Special emphasis is given to the physico‐chemical properties of CaP that promote osteogenesis, angiogenesis and provide sufficient mechanical strength for load‐bearing applications. Finally, the future course of action is discussed to ensure efficient utilization of CaP in the in vitro bone model development field.

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Effect of Biphasic Calcium Phosphate Treated with Vascular Endothelial Growth Factor on Osteogenesis and Angiogenesis Gene Expression In Vitro

Cited by 3 publications

References 17 publications

Enhanced Osteogenic and Angiogenic-Related Gene Expression of Human Dental Stem Cells on Biphasic Calcium Phosphate Scaffold Treated with Vascular Endothelial Growth Factor: Part I

Enhanced Osteogenic and Angiogenic-Related Gene Expression of Human Dental Stem Cells on Biphasic Calcium Phosphate Scaffold Treated with Vascular Endothelial Growth Factor: Part I

Challenges and Opportunities for Online Learning in India

Calcium Phosphates: A Key to Next‐Generation In Vitro Bone Modeling

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