Effect of Biochemical Components on Rheologic Properties of Nasal Mucus in Chronic Sinusitis

Abstract

The effect of biochemical components on the viscoelasticity of nasal mucus from 24 patients with chronic sinusitis (CS) was investigated by multiple stepwise regression analysis. The dynamic viscosity (eta') and the elastic modulus (G') of nasal mucus were determined with an oscillating sphere magnetic rheometer at oscillatory frequencies of 1 and 10 Hz. The eta' and G' values of mucus determined at 1 Hz were 1.6 +/- 1.5 Pa/s and 31.8 +/- 31.0 Pa, respectively, and these values were much higher than optimal viscoelasticity for mucociliary transport. The concentrations of fucose, N-acetyl neuraminic acid, albumin, IgG, secretory-IgA, and lysozyme were measured in the same mucus samples. The multiple regression analysis showed that the concentration of fucose, a marker of mucous glycoproteins, was the most important determinant of eta' and G'. The analysis also revealed that the level of IgG was the next important determinant. The coefficients of multiple determination for fucose and IgG were 0.732 and 0.733 when the response variables were eta' and G', respectively. The results indicate that locally produced mucous glycoproteins may largely contribute to the high viscoelasticity of nasal mucus in CS.

Efeitos agudos do ultrassom terapêutico de 1-MHz na desobstrução nasal de indivíduos com rinossinusite crônica

“…25 This correlates with our findings, as VSEx values increased significantly, which suggests that there was a mucolytic effect that resulted in elimination of nasal secretions by lavage, as observed in patients with chronic rhinosinusitis. [25][26][27][28] Similarly, the observed effects on VSEx and VAEx and on other symptoms of chronic rhinosinusitis may have taken place because of an increased local temperature -the known thermal effects of continuous LIUST. These effects also change the viscoelastic (rheologic) properties of the nasosinusal mucus by breaking the disulphide bonds of mucoproteins [26][27][28] thereby changing the fluidity of nasal secretions, which therefore drain more easily after nasal lavage in the LIUST-treated group.…”

TNFα modifica la respuesta de la frecuencia de batido ciliar a la carga viscosa, asociado a alteraciones en el calcio intracelular en células ciliadas respiratorias humanas

“…En enfermedades crónicas de vía aérea superior e inferior como el caso de la rinosinusitis crónica se ha observado un aumento en la viscosidad de las secreciones mucosas con un promedio de 16 poise 22 . Sin embargo no existen estudios in vitro que evalúen el efecto de la suma de niveles elevados de una citoquina proinflamatoria y aumento de la carga viscosa sobre la FBC de las células del epitelio respiratorio.…”

Métodos de estudo do transporte mucociliar

“…These observations make room for the development of new drugs for the treatment of over secretion of mucus and to better understand the pharmacologic actions of those already in the market [16][17][18] .…”

“…In chronic bronchitis and cystic fibrosis, the main factors responsible for an increase in viscoelasticity are the DNA derived from neutrophils and Actin-F. Although we do not know the exact mechanisms through which IgG increases viscoelasticity, controlling inflammatory processes reduces viscoelasticity in parallel to the drop in IgG levels in the mucus 17 .…”