Effect of binding of fibrinogen to each bacterium on coaggregation between <i>Porphymmonas gingivalis</i> and <i>Streptococcus oralis</i>

Nagata, Hideki; Amano, Atsuo; Ojima, Miki; Tanaka, Muneo; Kataoka, Kiyonori; Shizukuishi, Satoshi

doi:10.1111/j.1399-302x.1994.tb00286.x

Cited by 18 publications

(15 citation statements)

References 24 publications

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“…A number of salivary components are capable of interacting with P. gingivalis. 14 Several components such as fibrinogen, 15 histatins, 16 and fibronectin 17 can inhibit bacterial adherence. On the other hand, salivary proline-rich proteins (PRPs)/proline-rich glycoproteins (PRGs) and statherin were found to act as salivary receptors for the organism as well as several other plaque-forming bacteria.…”

Section: In the Oral Cavitymentioning

confidence: 99%

Molecular Interaction of Porphyromonas gingivalis with Host Cells: Implication for the Microbial Pathogenesis of Periodontal Disease

Amano

2003

Journal of Periodontology

174

139

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Porphyromonas gingivalis is a predominant periodontal pathogen, which expresses a number of potential virulence factors involved in the pathogenesis of periodontitis. Among them, fimbriae are a critical factor to mediate the bacterial interaction with host tissues, which promotes the bacterial adhesion to and invasion of the targeted sites. Fimbriae are capable of binding to human salivary components, commensal bacteria, and a variety of host cells including macrophages, epithelial cells, and fibroblasts. Human extracellular matrix (ECM) proteins such as vitronectin and fibronectin play important roles in cellular signal transduction via binding to receptor integrins. Fimbriae showed significant binding affinity to ECM proteins and clearly inhibited the molecular interactions between vitronectin/fibronectin and their receptor alphavbeta3 and alpha5beta1 integrins overexpressed on Chinese hamster ovary (CHO) cell strain. P. gingivalis fimbriae are likely to interrupt the cellular signaling via ECM proteins/integrins in periodontal regions. Fimbriae are also thought to be critically important in invasive events of the organism to host cells. The fimA genes, encoding FimA (a subunit of fimbriae), of P. gingivalis strains are classified into 5 types, I to V. Recent clinical investigations demonstrated the close relationship between the organisms with type II fimA and periodontitis development. Recombinant FimA (rFimA) proteins of types I to V were generated to compare their adhesion/invasion abilities to human gingival fibroblasts (HGF) and a human epithelial cell line (HEp-2 cells), respectively. There were no significant differences in the adhesion ability of microspheres (MS) coated with these rFimAs to HGF; however, the adhesion of type II rFimA-MS to HEp-2 cells was significantly greater than that of other rFimA types. It was also observed that the type II rFimA-MS markedly invaded the epithelial cells and accumulated around the nuclei. Collectively, these findings suggest that fimbriae of P. gingivalis, especially type II, are involved in the initiation and progression of human periodontitis.

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Section: In the Oral Cavitymentioning

confidence: 99%

Molecular Interaction of Porphyromonas gingivalis with Host Cells: Implication for the Microbial Pathogenesis of Periodontal Disease

Amano

2003

Journal of Periodontology

174

139

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“…Molecules present in these fluids that are bound by bacteria have the potential to modulate co-adherence. Salivary molecules including lysozyme and histatins, together with serum molecules such as fibrinogen, can inhibit adhesion of S. mitis, S. oralis, or S. crista to P. gingivalis (Murakami et al, 1991;Nagata et al, 1994). Hence, these molecules may comprise part of a host defense mechanism against colonization by P. gingivalis.…”

Section: Streptococcal Adhesion To Other Cellsmentioning

confidence: 99%

Streptococcal Adhesion and Colonization

Jenkinson

Lamont

1997

Critical Reviews in Oral Biology & Medicine

243

187

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Streptococci express arrays of adhesins on their cell surfaces that facilitate adherence to substrates present in their natural environment within the mammalian host. A consequence of such promiscuous binding ability is that streptococcal cells may adhere simultaneously to a spectrum of substrates, including salivary glycoproteins, extracellular matrix and serum components, host cells, and other microbial cells. The multiplicity of streptococcal adherence interactions accounts, at least in part, for their success in colonizing the oral and epithelial surfaces of humans. Adhesion facilitates colonization and may be a precursor to tissue invasion and immune modulation, events that presage the development of disease. Many of the streptococcal adhesins and virulence-related factors are cell-wall-associated proteins containing repeated sequence blocks of amino acids. Linear sequences, both within the blocks and within non-repetitive regions of the proteins, have been implicated in substrate binding. Sequences and functions of these proteins among the streptococci have become assorted through gene duplication and horizontal transfer between bacterial populations. Several adhesins identified and characterized through in vitro binding assays have been analyzed for in vivo expression and function by means of animal models used for colonization and virulence. Information on the molecular structure of adhesins as related to their in vivo function will allow for the rational design of novel acellular vaccines, recombinant antibodies, and adhesion agonists for the future control or prevention of streptococcal colonization and streptococcal diseases.

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“…S chenkein & G enco (37) demonstrated that components in plasma show a resemblance to those in crevicular fluid. Plasma has been shown to intensively inhibit coaggregation between P. gingivalis and S. mitis or S. oralis (14, 29). Thus, the effect of plasma on individual interaction among bacterial species tested was investigated.…”

Section: Discussionmentioning

confidence: 99%

“…N agata et al. (14) showed that fibrinogen and/or its fragments mask the sites on P. gingivalis cells that are responsible for coaggregation with S. oralis . It is therefore considered that fibrinogen is one of the most potent inhibitors for interactions among bacterial species tested.…”

Section: Discussionmentioning

confidence: 99%

Influence of secondary colonizers and human plasma on the adherence of Porphyromonas gingivalis in vitro

Satô¹,

Nagayama²,

Yamaguchi³

et al. 2002

European J Oral Sciences

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The influence of secondary colonizers (Fusobacterium nucleatum and Actinomyces naeslundii) and the effect of human plasma on the adherence of Porphyromonas gingivalis were investigated. Hydroxyapatite (HAP) discs coated with Streptococcus sanguis were immersed in a 3H-labeled bacterial cell suspension of F. nucleatum or A. naeslundii and then in a 14C-labeled P. gingivalis cell suspension. Bacterial cells on the discs were pyrolysed to quantify the radioisotopes released. The cell numbers of secondary colonizers on the discs increased with immersion time and this, in turn, resulted in significantly elevated adherence of P. gingivalis. These two secondary colonizers had very similar positive effects on the adherence of P. gingivalis. Human plasma significantly inhibited the adherence of P. gingivalis and secondary colonizers to S. sanguis-coated HAP discs. Adherence of P. gingivalis and A. naeslundii was strongly inhibited by plasma, while that of F. nucleatum was affected the least. Treatment with plasma, after immersion of streptococcal-coated discs in individual cell suspension of secondary colonizers, also reduced subsequent adherence of P. gingivalis. The rate of decrease was much smaller in F. nucleatum. These results indicate that both F. nucleatum and A. naeslundii enhance the adherence of P. gingivalis, and that the former may play a more important role in the establishment of P. gingivalis in dental plaque where plasma-derived components are present.

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Effect of binding of fibrinogen to each bacterium on coaggregation between Porphymmonas gingivalis and Streptococcus oralis

Cited by 18 publications

References 24 publications

Molecular Interaction of Porphyromonas gingivalis with Host Cells: Implication for the Microbial Pathogenesis of Periodontal Disease

Molecular Interaction of Porphyromonas gingivalis with Host Cells: Implication for the Microbial Pathogenesis of Periodontal Disease

Streptococcal Adhesion and Colonization

Influence of secondary colonizers and human plasma on the adherence of Porphyromonas gingivalis in vitro

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