Effect of Benincasa hispida fruits on testosterone-induced prostatic hypertrophy in albino rats

Nandecha, Chetan; Nahata, Alok; Dixit, Vinod Kumar

doi:10.1016/j.curtheres.2010.10.006

Cited by 27 publications

(25 citation statements)

References 20 publications

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“…Nandecha et al . () demonstrated the effects of BH against testosterone‐induced prostatic hypertrophy in albino rats and also determined the in vitro 5α‐reductase inhibitory activity of BH extracts using HPLC. Thus, the antiandrogenic properties of this plant make it a strong candidate for the present screening.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of 5α-reductase inhibitory activity of certain herbs useful as antiandrogens

Nahata

Dixit

2013

Andrologia

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This study demonstrates 5α-reductase inhibitory activity of certain herbs useful in the management of androgenic disorders. Ganoderma lucidum (Curtis) P. Karst (GL), Urtica dioica Linn. (UD), Caesalpinia bonducella Fleming. (CB), Tribulus terrestris Linn. (TT), Pedalium murex Linn. (PM), Sphaeranthus indicus Linn. (SI), Cuscuta reflexa Roxb. (CR), Citrullus colocynthis Schrad. (CC), Benincasa hispida Cogn. (BH), Phyllanthus niruri Linn. (PN) and Echinops echinatus Linn. (EE) were included in the study. Petroleum ether, ethanol and aqueous extracts of these herbs were tested for their 5α-reductase inhibitory activity against the standard 5α-reductase inhibitor, finasteride. A biochemical method to determine the activity of 5α-reductase was used to evaluate the inhibition of different extracts to the enzyme. The optical density (OD) value of each sample was measured continuously with ultraviolet spectrophotometer for the reason that the substrate NADPH has a specific absorbance at 340 nm. As the enzyme 5α-reductase uses NADPH as a substrate, so in the presence of 5α-reductase inhibitor, the NADPH concentration will increase with the function of time. This method thus implicates the activity of 5α-reductase. The method proved to be extremely useful to screen the herbs for their 5α-reductase inhibitory potential. GL, UD, BH, SI and CR came out to be promising candidates for further exploring their antiandrogenic properties.

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Section: Methodsmentioning

confidence: 99%

Evaluation of 5α-reductase inhibitory activity of certain herbs useful as antiandrogens

Nahata

Dixit

2013

Andrologia

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“…Human prostate (about 200 mg) procured from the local hospital of Sagar was minced in small pieces and homogenized in 10 mL of medium A (20 m m sodium phosphate, pH 6.5, containing 0.32 m m sucrose and 1 m m EDTA) as described previously (Pandit et al , ; Nandecha et al , ; Nahata and Dixit, ). The homogenate was centrifuged at 4000 rpm (716 × g ) for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Sphaeranthus indicus Attenuates Testosterone induced Prostatic Hypertrophy in Albino Rats

Nahata

Dixit

2011

Phytotherapy Research

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The present study reports the attenuating effect of Sphaeranthus indicus extracts (SI) on prostatic hyperplasia induced by testosterone in albino rats. In vitro studies were conducted to assess the 5α-reductase inhibitory potential of the petroleum ether, ethanolic and aqueous extracts of SI. A biochemical marker, β-sitosterol, was isolated and extracts were characterized utilizing HPTLC. Testosterone (3 mg/kg s.c.) was administered to the rats along with the test extracts and isolated β-sitosterol for a period of 28 days. The weight of the rats, the urine output, serum testosterone concentrations and prostate-specific antigen (PSA) levels were recorded. The prostate/body weight ratio (P/BW) was calculated and histological studies were performed to observe the changes in the histoarchitecture of the prostate. Finasteride was used as a positive control (1 mg/kg p.o.). Sphaeranthus indicus extracts attenuated the increase in the P/BW ratio induced by testosterone in the treated groups. The petroleum ether extract exhibited the best activity, although the ethanol and aqueous extracts also exhibited significant activity. Urine output was also improved significantly, demonstrating the clinical implications of the study. Histological studies, testosterone levels which were measured weekly and PSA levels measured at the end of the study also support claims for the potential use of Sphaeranthus indicus in the treatment of prostatic hyperplasia.

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“…Dihydrotestosterone is formed from testosterone by the enzyme 5α‐reductase which is present in prostate homogenates (Dhanotiya et al ., ; Nandecha et al ., ). Nahata & Dixit () reported that finasteride is about 10 times more potent in inhibiting 5α‐reductase activity in vitro .…”

Section: Discussionmentioning

confidence: 97%

Preventive effect ofPueraria mirificaon testosterone-induced prostatic hyperplasia in Sprague Dawley rats

Masrudin

Mohamad

2015

Andrologia

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Pueraria mirifica (PM) extract contains phytoestrogen daidzein and genistein. In this study, we investigated the protective effect of PM extract, daidzein and genistein on a testosterone-induced prostatic hyperplasia in rats. Testosterone was administered at 3 mg kg(-1) to rats followed by the PM extract, daidzein and genistein for a period of 30 days with finasteride as positive control. The testosterone level was increased, indicating inhibition of 5α-reductase converting testosterone to dihydrotestosterone. This was confirmed by prostate-specific antigen level that significantly decreased when treated with PM extract, daidzein and genistein. The PM extract, daidzein and genistein reduced the increase in the prostate/body weight ratio in testosterone-induced rats. This gives indication that PM extract, daidzein and genistein possessed protective activity for the treatment of benign prostatic hyperplasia. The analysis of histoarchitechture of the prostate has also shown that there was a significant improvement in prostatic cells of the testosterone-induced rats when treated with PM extract, daidzein and genistein.

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Effect of Benincasa hispida fruits on testosterone-induced prostatic hypertrophy in albino rats

Abstract: Petroleum ether extract and B hispida seed oil inhibited testosterone-induced hyperplasia of the prostate in these rats. Further studies are needed to evaluate its effect in humans with benign prostatic hyperplasia.

Cited by 27 publications

References 20 publications

Evaluation of 5α-reductase inhibitory activity of certain herbs useful as antiandrogens

Evaluation of 5α-reductase inhibitory activity of certain herbs useful as antiandrogens

Sphaeranthus indicus Attenuates Testosterone induced Prostatic Hypertrophy in Albino Rats

Preventive effect ofPueraria mirificaon testosterone-induced prostatic hyperplasia in Sprague Dawley rats

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