Preventive effect ofPueraria mirificaon testosterone-induced prostatic hyperplasia in Sprague Dawley rats

Abstract

Pueraria mirifica (PM) extract contains phytoestrogen daidzein and genistein. In this study, we investigated the protective effect of PM extract, daidzein and genistein on a testosterone-induced prostatic hyperplasia in rats. Testosterone was administered at 3 mg kg(-1) to rats followed by the PM extract, daidzein and genistein for a period of 30 days with finasteride as positive control. The testosterone level was increased, indicating inhibition of 5α-reductase converting testosterone to dihydrotestosterone. This was confirmed by prostate-specific antigen level that significantly decreased when treated with PM extract, daidzein and genistein. The PM extract, daidzein and genistein reduced the increase in the prostate/body weight ratio in testosterone-induced rats. This gives indication that PM extract, daidzein and genistein possessed protective activity for the treatment of benign prostatic hyperplasia. The analysis of histoarchitechture of the prostate has also shown that there was a significant improvement in prostatic cells of the testosterone-induced rats when treated with PM extract, daidzein and genistein.

Risks and benefits related to alimentary exposure to xenoestrogens

“…Apigenin displayed a very interesting pharmacological profile and can now be considered as a lead compound for targeting prostate cancer (Singh et al, 2014). Rat with testosterone-induced prostatic hyperplasia were treated with Pueraria mirifica (PM) extract, which contains daidzenin and genistein, and a reduction of the increase in the prostate/body weight ratio was found after treatment, showing a significant inhibition of benign prostatic hyperplasia (Masrudin and Mohamad, 2014). These two compounds were found also to be efficient in reversing DNA methylation in prostate cancer cell lines, such as PC3, DU145 and LNCaP.…”

Inhibition of α1-Adrenergic, Non-Adrenergic and Neurogenic Human Prostate Smooth Muscle Contraction and of Stromal Cell Growth by the Isoflavones Genistein and Daidzein

“…Clinical observations confirmed possible effects of isoflavones on BPH/LUTSs, as preparations from isoflavone-rich plants partly reduced symptoms and the risk for symptomatic BPH [ 37 ]. However, previous experimental studies addressing isoflavone impacts in the prostate focused on estrogenic effects, hormone-disrupting properties, epithelial prostate cells, animal models and prostate cancer [ 38 , 39 , 40 , 41 , 42 , 43 ]. Consequently, the effects of isoflavones on human prostate smooth muscle contraction and on the growth of stromal cells are mostly unknown, and directed comparisons to identify the most active isoflavones in the prostate are still pending.…”

“…Our current findings, together with previous findings from epithelial cells, may suggest that genistein could reduce prostate growth in BPH in vivo, provided adequate concentrations occur in the prostate. In fact, genistein inhibited prostate growth in rodent models of testosterone-induced BPH and high-fat-induced prostate growth [ 38 , 40 , 43 ], as well as ex vivo growth in prostate tissues from patients with BPH [ 49 ].…”

Pentoxifylline mitigates detrimental impact of chronic nonbacterial prostatitis on sperm characteristics, reproductive hormones and histopathology in rats

“…However, the animals treated with cernilton and PTX showed mild leucocyte infiltration, focal epithelium proliferation and interstitial fibrosis in comparison with the CNP‐model group. The most prominent indicator for evaluation of prostate hyperplasia is PSA, which has a relationship with severity of prostate inflammation (Masrudin & Mohamad, ). Oral administration of cernilton and PTX in CNP + cernilton and CNP + PTX groups for 14 days resulted in a significant reduction in the PSA levels as compared to the CNP‐model group.…”