Effect of bag-in-the-lens implantation on posterior capsule opacification in human donor eyes and rabbit eyes

Groot, Veva De; Tassignon, Marie-José; Vrensen, Gijs F.J.M.

doi:10.1016/j.jcrs.2004.04.061

Cited by 37 publications

(24 citation statements)

References 31 publications

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“…14,19 -23 With respect to testing IOLs using such systems, the body of literature is limited. 14,19,22,24 Liu et al 14 initially demonstrated that an IOL could impede growth to some degree using the human capsular bag system developed in Norwich. In the present study, we made a modification in the protocol such that the capsular bag containing the IOL was inverted, resulting in the anterior capsule being closest to the plastic dish.…”

Section: Discussionmentioning

confidence: 99%

A Fully Human In Vitro Capsular Bag Model to Permit Intraocular Lens Evaluation

Dawes

Illingworth

Wormstone

2012

Invest. Ophthalmol. Vis. Sci.

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PURPOSE.To establish a fully human in vitro culture model with which to test the putative effects of intraocular lens (IOL) designs in preventing posterior capsule opacification (PCO) after cataract surgery. METHODS. A sham cataract operation was performed to prepare human capsular bags from donor lenses. In one capsular bag of a donor pair, an intraocular lens (PMMA round-edge IOL or acrylic IOL) was implanted while the other capsular bag remained aphakic. Bags were transferred to a Petri dish and secured anterior-face down using entomological pins. Capsular bags were maintained in Eagle's minimum essential medium supplemented with 2% human serum and 10 ng/mL TGF-␤ to drive growth and matrix contraction. RESULTS. In the absence of an IOL, cells appeared within the central posterior capsule at 4.38 Ϯ 0.26 days, whereas in the presence of a PMMA round-edge IOL or an acrylic IOL they appeared at 8 Ϯ 0.41 days and 11 Ϯ 0.7 days, respectively. Immunocytochemical analysis showed an accumulation of cells at the edge of the acrylic IOL and a less evident accumulation with the PMMA round-edge IOL. Moreover, matrix contraction was more prominent in the absence of an IOL but was still apparent, to a lesser degree, in the presence of a PMMA round-edge IOL. The acrylic IOL greatly suppressed matrix contraction. CONCLUSIONS. The authors have developed a fully human in vitro capsular bag system that relates well to clinical observations and permits the testing of novel intraocular lenses. (Invest Ophthalmol Vis Sci. 2012;53:23-29)

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Section: Discussionmentioning

confidence: 99%

A Fully Human In Vitro Capsular Bag Model to Permit Intraocular Lens Evaluation

Dawes

Illingworth

Wormstone

2012

Invest. Ophthalmol. Vis. Sci.

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“…The development of capsular bag models allowed for the introduction of an IOL (Cleary et al, 2010;Dawes et al, 2012;De Groot et al, 2005;Duncan et al, 1997;Eldred et al, 2014;Liu et al, 1996) and thus provide an alternative experimental system for IOL development and importantly permit IOLs to be tested in a human system. For example, the capsular bag model served as an invaluable tool in the development of the Bag-in-the-lens IOL developed by Prof. Tassignon (De Groot et al, 2005), which is now commercially available. Pedagogic changes have been made to the model that have further improved its value in IOL development.…”

Section: Capsular Bagsmentioning

confidence: 99%

Experimental models for posterior capsule opacification research

Wormstone

Eldred

2016

Experimental Eye Research

103

102

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Millions of people worldwide are blinded due to cataract formation. At present the only means of treating a cataract is through surgical intervention. A modern cataract operation involves the creation of an opening in the anterior lens capsule to allow access to the fibre cells, which are then removed. This leaves in place a capsular bag that comprises the remaining anterior capsule and the entire posterior capsule. In most cases, an intraocular lens is implanted into the capsular bag during surgery. This procedure initially generates good visual restoration, but unfortunately, residual lens epithelial cells undergo a wound-healing response invoked by surgery, which in time commonly results in a secondary loss of vision. This condition is known as posterior capsule opacification (PCO) and exhibits classical features of fibrosis, including hyperproliferation, migration, matrix deposition, matrix contraction and transdifferentiation into myofibroblasts. These changes alone can cause visual deterioration, but in a significant number of cases, fibre differentiation is also observed, which gives rise to Soemmering's ring and Elschnig's pearl formation. Elucidating the regulatory factors that govern these events is fundamental in the drive to develop future strategies to prevent or delay visual deterioration resulting from PCO. A range of experimental platforms are available for the study of PCO that range from in vivo animal models to in vitro human cell and tissue culture models. In the current review, we will highlight some of the experimental models used in PCO research and provide examples of key findings that have resulted from these approaches.

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“…There may also be a place for modified implantation techniques and improved IOL designs. 70 Childhood cataract remains an important but treatable cause of potentially lifelong visual handicap. Those privileged to work with affected children have a duty to provide them with the best possible care.…”

Section: Complicationsmentioning

confidence: 99%