Effect of baculovirus infection on the mRNA and protein levels of the <i>Spodoptera frugiperda</i> eukaryotic initiation factor 4E

Oers, Monique M. van; Veken, L. T. J. N. van der; Vlak, Just M.; Thomas, Adri A. M.

doi:10.1046/j.1365-2583.2001.00263.x

Cited by 19 publications

(21 citation statements)

References 49 publications

(110 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Concomitant with the onset of the very late phase (18 hpi–), a pronounced down–regulation of host transcriptional activity occurs [10], followed by a shut off of most protein synthesis by 24 hpi [11]. This phenomenon reflects increased viral autonomy during the later phases of infection, confirmed by the observation that translation of the very-late genes p10 and polh is relatively insensitive to the presence of 5′-cap-binding eukaryotic initiation factor eIF4E [12], [13].…”

Section: Introductionmentioning

confidence: 91%

Quantitative Proteomics of Spodoptera frugiperda Cells during Growth and Baculovirus Infection

et al. 2011

View full text Add to dashboard Cite

Baculovirus infection of Spodoptera frugiperda cells is a system of choice to produce a range of recombinant proteins, vaccines and, potentially, gene therapy vectors. While baculovirus genomes are well characterized, the genome of S. frugiperda is not sequenced and the virus-host molecular interplay is sparsely known. Herein, we describe the application of stable isotope labeling by amino acids in cell culture (SILAC) to obtain the first comparative proteome quantitation of S. frugiperda cells during growth and early baculovirus infection. The proteome coverage was maximized by compiling a search database with protein annotations from insect species. Of interest were differentially proteins related to energy metabolism, endoplasmic reticulum and oxidative stress, yet not investigated in the scope of baculovirus infection. Further, the reduced expression of key viral-encoded proteins early in the infection cycle is suggested to be related with decreased viral replication at high cell density culture. These findings have implications for virological research and improvement of baculovirus-based bioprocesses.

show abstract

Section: Introductionmentioning

confidence: 91%

Quantitative Proteomics of Spodoptera frugiperda Cells during Growth and Baculovirus Infection

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Several mechanisms allowing baculovirus mRNAs to compete with insect host mRNAs for translation have been found. These include the presence of AT-rich regions and unstructured 5' untranslated (UTR) regions [8,98], which confer a higher binding affinity for host translation factors by virus mRNAs compared to that of host mRNAs; the relative independence of virus late mRNAs on the 5'-cap-binding eukaryotic initiation factor (eIF4E) for the ribosomal recruitment process, in contrast to most insect mRNAs, which are dependent on cap-binding proteins for being translated [98,99]; and the ability of several virus genes, namely protein kinase 2 (pk-2) and host range factor 1 (hrf-1), to inhibit translational shutoff [64,66]. Emerging evidence also suggests that baculoviruses up-regulate translation initiation factors to accelerate the translation process [7,11].…”

Section: Host Genes/pathways That Baculoviruses Require For Succesmentioning

confidence: 99%

Genome Scale Transcriptomics of Baculovirus-Insect Interactions

Nguyen

Nielsen

Reid

2013

Viruses

View full text Add to dashboard Cite

Baculovirus-insect cell technologies are applied in the production of complex proteins, veterinary and human vaccines, gene delivery vectors‚ and biopesticides. Better understanding of how baculoviruses and insect cells interact would facilitate baculovirus-based production. While complete genomic sequences are available for over 58 baculovirus species, little insect genomic information is known. The release of the Bombyx mori and Plutella xylostella genomes, the accumulation of EST sequences for several Lepidopteran species, and especially the availability of two genome-scale analysis tools, namely oligonucleotide microarrays and next generation sequencing (NGS), have facilitated expression studies to generate a rich picture of insect gene responses to baculovirus infections. This review presents current knowledge on the interaction dynamics of the baculovirus-insect system‚ which is relatively well studied in relation to nucleocapsid transportation, apoptosis, and heat shock responses, but is still poorly understood regarding responses involved in pro-survival pathways, DNA damage pathways, protein degradation, translation, signaling pathways, RNAi pathways, and importantly metabolic pathways for energy, nucleotide and amino acid production. We discuss how the two genome-scale transcriptomic tools can be applied for studying such pathways and suggest that proteomics and metabolomics can produce complementary findings to transcriptomic studies.

show abstract

“…By 24 hpi, the majority of newly synthesized proteins are either virus-encoded or virus-induced. The mechanism responsible for the shut off of host protein synthesis is not fully understood but appears to correlate with a reduction in host gene transcripts (Ooi and Miller, 1988; van Oers et al, 2003; van Oers et al, 2001b). A differential display approach was used to identify potentially up-regulated host genes (Nobiron et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Comprehensive analysis of host gene expression in Autographa californica nucleopolyhedrovirus-infected Spodoptera frugiperda cells

et al. 2011

View full text Add to dashboard Cite

Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) is the best studied baculovirus and most commonly used virus vector for baculovirus expression vector systems. The effect of AcMNPV infection on host cells is incompletely understood. A microarray based on Spodoptera frugiperda ESTs was used to investigate the impact of AcMNPV on host gene expression in cultured S. frugiperda, Sf21 cells. Most host genes were down regulated over the time course of infection, although a small number were up-regulated. The most highly up-regulated genes encoded heat shock protein 70s and several poorly characterized proteins. Regulated genes with the highest score identified by functional annotation clustering included primarily products required for protein expression and trafficking in the ER and golgi. All were significantly down-regulated by approximately 12 hours post infection. Microarray data were validated by qRT-PCR. This study provides the first comprehensive host transcriptome overview of Sf21 cells during AcMNPV infection.

show abstract

Effect of baculovirus infection on the mRNA and protein levels of the Spodoptera frugiperda eukaryotic initiation factor 4E

Cited by 19 publications

References 49 publications

Quantitative Proteomics of Spodoptera frugiperda Cells during Growth and Baculovirus Infection

Quantitative Proteomics of Spodoptera frugiperda Cells during Growth and Baculovirus Infection

Genome Scale Transcriptomics of Baculovirus-Insect Interactions

Comprehensive analysis of host gene expression in Autographa californica nucleopolyhedrovirus-infected Spodoptera frugiperda cells

Contact Info

Product

Resources

About