Comprehensive analysis of host gene expression in Autographa californica nucleopolyhedrovirus-infected Spodoptera frugiperda cells

Salem, Tamer Z.; Zhang, Fengrui; Xie, Yan; Thiem, Suzanne M.

doi:10.1016/j.virol.2011.01.006

Cited by 61 publications

(73 citation statements)

References 65 publications

(78 reference statements)

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“…The target genes of some of these miRNAs are linked to processes such as translation elongation and cellular protein complex assembly. Salem et al (2011) reported downregulation of genes involved in protein expression and trafficking after 12 h of AcMNPV infection in Sf21 cells. Thus, there seems to be a high correlation between these studies, and the role of baculoviral miRNAs in translational control should be investigated.…”

Section: Expression Profiling Of Spltnpv Mirnas At Different Time Intmentioning

confidence: 99%

Temporal expression profiling of novel Spodoptera litura nucleopolyhedrovirus-encoded microRNAs upon infection of Sf21 cells

Kharbanda

Jalali

Ojha

et al. 2015

Journal of General Virology

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Section: Expression Profiling Of Spltnpv Mirnas At Different Time Intmentioning

confidence: 99%

Temporal expression profiling of novel Spodoptera litura nucleopolyhedrovirus-encoded microRNAs upon infection of Sf21 cells

Kharbanda

Jalali

Ojha

et al. 2015

Journal of General Virology

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“…In addition, in a prior microarray study, three hsp70 genes were reported to be differentially regulated in Sf9 cells infected with AcMNPV (52). While one hsp70 transcript was gradually downregulated throughout the infection, two other hsp70 genes were dramatically upregulated at 6 h p.i., and the degree of upregulation declined as infection progressed (52).…”

Section: Assembly and Annotation Of The T Ni Tnms42 Transcriptomementioning

confidence: 98%

Transcriptome Responses of the Host Trichoplusia ni to Infection by the Baculovirus Autographa californica Multiple Nucleopolyhedrovirus

Chen

Zhong

Fei

et al. 2014

J Virol

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Productive infection of Trichoplusia ni cells by the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) leads to expression of ϳ156 viral genes and results in dramatic cell remodeling. How the cell transcriptome responds to viral infection was unknown due to the lack of a reference genome and transcriptome for T. ni. We used an ϳ60-Gb RNA sequencing (RNA-seq) data set from infected and uninfected T. ni cells to generate and annotate a de novo transcriptome assembly of approximately 70,322 T. ni unigenes (assembled transcripts), representing the 48-h infection cycle. Using differential gene expression analysis, we found that the majority of host transcripts were downregulated after 6 h postinfection (p.i.) and throughout the remainder of the infection. In contrast, 5.7% (4,028) of the T. ni unigenes were upregulated during the early period (0 to 6 h p.i.), followed by a decrease through the remainder of the infection cycle. Also, a small subset of genes related to metabolism and stress response showed a significant elevation of transcript levels at 18 and 24 h p.i. but a decrease thereafter. We also examined the responses of genes belonging to a number of specific pathways of interest, including stress responses, apoptosis, immunity, and protein trafficking. We identified specific pathway members that were upregulated during the early phase of the infection. Combined with the parallel analysis of AcMNPV expression, these results provide both a broad and a detailed view of how baculovirus infection impacts the host cell transcriptome to evade cellular defensive responses, to modify cellular biosynthetic pathways, and to remodel cell structure. IMPORTANCEBaculoviruses are insect-specific DNA viruses that are highly pathogenic to their insect hosts. In addition to their use for biological control of certain insects, baculoviruses also serve as viral vectors for numerous biotechnological applications, such as mammalian cell transduction and protein expression for vaccine production. While there is considerable information regarding viral gene expression in infected cells, little is known regarding responses of the host cell to baculovirus infection. In these studies, we assembled a cell transcriptome from the host Trichoplusia ni and used that transcriptome to analyze changes in host cell gene expression throughout the infection cycle. The study was performed in parallel with a prior study of changes in viral gene expression. Combined, these studies provide an unprecedented new level of detail and an overview of events in the infection cycle, and they will stimulate new experimental approaches to understand, modify, and utilize baculoviruses for a variety of applications.

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“…The decrease in host transferrin expression mediated by SpliNPV infection could be explained by different mechanisms. SpliNPV could repress the expression of host transferrin due to a shut-off mechanism of host protein synthesis similar to that shown in Spodoptera frugiperda (Salem et al, 2011). Baculovirus-infected host protein synthesis is shut down at approximately 10-12 h after infection when the virus starts producing new virions (Carstens et al, 1979;Maruniak et al, 1981).…”

Section: Discussionmentioning

confidence: 60%

Transcriptional profiling of transferrin gene from Egyptian cotton leaf worm, Spodoptera littoralis

Güz¹,

Dageri²,

Erdoğan³

et al. 2013

Turk J Biol

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Insect immune responses involved in virus defense have lately received increasing attention. While the pathways and effector molecules active in defense against bacteria and fungi are well studied (Ferrandon et al., 2007;Levitin et al., 2008), the regulation of the innate immune system against viral infections still remains to be elucidated. The baculoviruses (Baculoviridae) are doublestranded DNA viruses that infect arthropods, mainly insects and in particular Lepidoptera (Cory and Myers, 2003). Baculoviruses are not only biocontrol agents of lepidopteran pests, but also have been developed and used extensively in research, serving as expression vectors for high-level production of recombinant proteins (Bonning and Hammock, 1996;Susurluk et al., 2013). Although baculoviruses have been successfully used to control lepidopteran and hymenopteran insect pests of agriculture and forestry importance worldwide, little is known about the host immune responses towards these viral infections (Federici, 1986;Miller, 1997; Moscardi, 1999).Transferrin is an iron-binding protein that has a role in iron transport (Nichol et al., 2002), in preventing oxidative stress, and in delivering iron to eggs for development (Yoshiga et al., 1997). In addition, it is known that transferrin synthesis is increased following exposure to bacteria, fungi, pathogens, and parasites, as well as insect parasitoids (

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Comprehensive analysis of host gene expression in Autographa californica nucleopolyhedrovirus-infected Spodoptera frugiperda cells

Cited by 61 publications

References 65 publications

Temporal expression profiling of novel Spodoptera litura nucleopolyhedrovirus-encoded microRNAs upon infection of Sf21 cells

Temporal expression profiling of novel Spodoptera litura nucleopolyhedrovirus-encoded microRNAs upon infection of Sf21 cells

Transcriptome Responses of the Host Trichoplusia ni to Infection by the Baculovirus Autographa californica Multiple Nucleopolyhedrovirus

Transcriptional profiling of transferrin gene from Egyptian cotton leaf worm, Spodoptera littoralis

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