“…As the temperature rises, acidification may occur when the water becomes warm, and the pH value gradually decreases [ 24 ]. At the initial stage of the breeding period, the farmers added artificial microecologics similar to probiotics to regulate the water quality, but the pH levels were high due to the inappropriate amount of microecologics used [ 25 ]. The pH values in the ponds began to drop between Z1 and Z2 because of the release of largemouth bass and other species for polyculture that increased the culture density, and a large amount of carbon dioxide was released through respiration, which pulled down the pH values [ 26 ].…”