Effect of Auxins on Adventitious Root Development from Single Node Cuttings of Camellia sinensis (L.) Kuntze and Associated Biochemical Changes

Abstract

An attempt was made to induce rooting from single node cuttings of Camellia sinensis var. TV-20 under controlled conditions and study its biochemical changes during rooting. The nodal cuttings were pretreated with different concentrations of IAA, NAA and IBA and kept in a growth chamber (25 ±2°C, 16 h photoperiod (55 lmol m À2 s À1 ) with cool, white fluorescent lamps and 65% relative humidity) for 12 h. Among the three auxins used for pretreatment, IBA showed more positive response on rooting as compared to IAA and NAA within 2 weeks of transfer to potting medium. Among four concentrations of IBA tested, 75 ppm gave maximum percentage of rooting, number of roots and root length. Therefore, IBA was used further in experiments for biochemical investigation. The adventitious rooting was obtained in three distinct phases i.e. induction (0-12 days), initiation (12-14 days) and expression (14-18 days). IAA-oxidase activity of IBAtreated cuttings increased slightly as compared to control. The activity was found to decrease during induction and initiation phases and increase during expression phase. The peroxidase activity in IBA-treated cuttings increased up to initiation phase and declined at the expression phase. Polyphenoloxidase activity increased both in IBA-treated and control cuttings during induction and initiation phase but declined slowly during expression phase. Total phenolic content was higher in IBA-treated cuttings, particularly in initiation and expression phases and it also correlated with peroxidase activity. Phenolics might be playing key role for induction of adventitious rooting, and phenolic compounds can be used as rooting enhancer in tea plant.Abbreviations: IAA -indole-3-acetic acid; IBA -indole-3-butyric acid; NAA -1-naphthaleneacetic acid

In vitro propagation of orchid (Dendrobium nobile) var. Emma white

“…NAA produced less number of roots than IBA probably due to the reason that NAA accumulates and cannot be rapidly catabolized particularly at higher levels than that of IBA (Vuylsteker et al, 1997). In contrast to these results, Rout (2006) quoted that NAA promotes the number of roots by enhancing cell division in the root primordial.…”

Effects of cutting size and exogenous hormone treatment on rooting of shoot cuttings in Norway spruce [Picea abies (L.) Karst.]

“…Applying IBA may have an indirect influence by enhancing the speed of translocation and movement of carbohydrates to the base of cuttings and consequently stimulate rooting (Davies et al 1990;Aminah et al 1995), so the rooting rate may be enhanced by the IBA treatment (Aminah et al 1995). This may also be related to total phenolic content and peroxidase activity, which are higher in IBA-treated cuttings, particularly during the initiation and expression phases (Moncousin and Gaspar 1983;Rout 2006). Similar to IBA, NAA treatment promotes rooting in conifer stem cuttings (Copes and Mandel 2000;Ragonezi et al 2010), but in the present study we found that NAA may be an inhibitor, which is in accordance with a study on apples (De Klerk et al 1997).…”

A Combination of IBA and NAA Resulted in Better Rooting and Shoot Sprouting than Single Auxin on Malay Apple [Syzygium malaccense (L.) Merr. & Perry] Stem Cuttings

“…IAAO is an enzyme which catalyzes oxidation of IAA, or breaks down endogenous auxin (Hartmann, Kester, Davies Jr., & Geneve, 2010). The activity of POD is reported to be involved in an important step of root formation, during the metabolism of auxin and the process of lignification (Rout, 2006). PPO involved in metabolism of mono-and polyphenols during lignification process in plant cells (Khorsheduzzaman, Alam, Rahman, Mian, & Mian, 2010).…”