Effect of Auxin Levels on Shoot Formation with Different Embryo Tissues from a Cultivar and a Commercial Hybrid of Wheat (Triticum aestivum L.)

Елена, Егорова; Ginzo, H. D.

doi:10.1016/s0176-1617(88)80261-x

Cited by 16 publications

(6 citation statements)

References 6 publications

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“…In this study, 25 elite durum wheats and ®ve triticale genotypes were evaluated using three media: DW1 medium, which formed embryogenic calli for elite CIMMYT wheat genotypes (Bohorova et al 1995); DW2, which was the preferred medium for dierent durum wheat genotypes (Bennici 1986); and DW3 (modi®ed), used by Maddock et al (1983), for bread wheat callus culture. The three media used are based on MS medium and are supplemented with 2,4-D (2.5 mg/l for DW1, 2 mg/l for DW2 and 1 mg/l for DW3), a commonly used growth regulator in wheat tissue culture (O'Hara and Street 1978, Carman et al 1988, Elena and Ginzo 1988. Two media contain two types of supplementary amino acids and dier in sucrose concentration (DW1 was supplemented with 60 g/l sucrose and DW2 with 25 g/l).…”

Section: Discussionmentioning

confidence: 99%

Regeneration potential of CIMMYT durum wheat and triticale varieties from immature embryos

Bohorova¹,

Pfeiffer²,

Mohamed³

et al. 2001

Plant Breeding

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Twenty‐five durum wheat elite advanced lines and released varieties, and five triticale varieties were evaluated for their ability to produce embryogenic callus using three different media. For callus initiation and maintenance there were basal Murashige and Skoog (MS) medium containing double strains of macroelements and 2.5 mg/l 2,4D (DW1), basal MS medium containing 2.0 mg/l 2,4D (DW2), or basal MS medium supplemented with 1.0 mg/l 2,4 D and coconut milk (DW3). Plant regeneration was achieved on basal MS medium with indoleacetic acid and 6‐benzylaminopurine, and plants rooted on MS with 1‐naphthale‐neacetic acid. DW3 medium proved better than the other media tested for embryogenic callus initiation and maintenance. Regeneration rates varied widely with both genotype and initiation medium, with values ranging from no regeneration to 100% regeneration; the plantlets produced per embryo ranged from five to 20. Fourteen of the durum wheat genotypes showed 63–100% regeneration from DW3 callus formation medium, four lines from DW1 medium, and two lines from DW2. Four of the triticale varieties had regeneration of 48–100% from DW3 medium. After six subcultures, over a 6‐month period, genotypes lost their ability to regenerate plants. Only 10 lines retained some plant regeneration potential but regeneration was at reduced levels. Successful regeneration of durum wheat and triticale varieties will be used as an integral part of the transformation process.

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Section: Discussionmentioning

confidence: 99%

Regeneration potential of CIMMYT durum wheat and triticale varieties from immature embryos

Bohorova¹,

Pfeiffer²,

Mohamed³

et al. 2001

Plant Breeding

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“…Similarly, more efficient callus induction and plant regeneration potential of young explants were also reported by several researchers [12,22,31,32]. Besides the type, amount of plant growth regulator in culture ambient has significant impacts in morphogenesis [33][34][35]. Such impacts might have also yielded different outcomes in different studies.…”

Section: Discussionmentioning

confidence: 68%

Effects of Cultivar and Explant Sources on Callus Induction and Plant Regeneration in Rice (Oryza sativa L.)

Akay¹,

Kurt²

2018

JAST-B

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In vitro callus induction and plant regeneration potentiality were studied from mature embryo of three Indian rice (Oryza sativa L.) groups at Field Crops Department, Agricultural Faculty, Ondokuz Mayıs University, Samsun, Turkey. The study was done by using callus induction MS medium having different concentration of four. The present research was conducted according to the design of randomized blocks trial. A total of 696 calluses, 193 plants and 917 seeds were obtained from Indica group; 2,110 calluses, 103 plants and 235 seeds were obtained from Japonica group; 1,243 calluses and 13 plants were obtained from Javanica group. With regard to number of calluses obtained from each explant source, 52 calluses were obtained from whole-plant explants, 1,668 calluses from root explants, 629 calluses from shoot explants, 649 calluses from the 1st node explants, 240 calluses from the 2nd node explants, 269 calluses from the 1st internode explants and 12 calluses from the 2nd internode explants. With regard to number of plants obtained from each explant source, 27 plants were obtained from whole-plant explants, 195 plants from shoot explants, 43 plants from the 1st node explants, 40 plants from the 2nd node explants and four plants from the 1st internode explants. With regard to number of seeds, 823 seeds were obtained from shoot explants and 329 seeds were obtained from the 2nd node explants. Germination rate of harvested seeds was over 90%. The establishment of this regeneration system is essential for the development of a genetic transformation system for commercial rice cultivars.

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“…A review of previous literature suggests variable success in tissue culture of different wheat species using callus of immature embryos (Becker et al 1994;Elena & Ginzo 1988;Maddock et al 1983;Weeks et al 1993) and mature embryos (Bi et al 2007;Patnaik et al 2006). Both plumule and radicle tissue initiated callus induction are affected by types of explant and plant species used in the study at all concentrations of 2,4-D. Callus weight and gain was cultivar and explant specific and largely inconsistent.…”

Section: Discussionmentioning

confidence: 99%

“…Development of transformation techniques has become one of the focal issues in wheat tissue culture and is becoming an important vehicle in wheat breeding programs throughout the world. It is making use of development of haploids, organogenesis, somatic embryogenesis, selection of somaclonal variants and production of transgenic plants for wheat improvement (Becker et al 1994;Bi et al 2007;Chu et al 1990;Elena & Ginzo 1988;Fellers et al 1995;Maddock et al 1983;Patnaik et al 2006;Rajyalakshmi et al 1991;Sarker & Biswas 2002;Weeks et al 1993). Previous reports suggested the effects of culture medium and its supplements (Mathias & Simpson 1986) tissue (Vasil 1994) and genotype (Hess & Carman 1998) on tissue culture of wheat.…”

Section: Introductionmentioning

confidence: 99%

Somatic Embryogenesis on Plumule and Radicle Explants Obtained from Warm Water Hydroprimed Wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds

Kahriz¹,

Kahriz²

2017

JSM

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Effect of Auxin Levels on Shoot Formation with Different Embryo Tissues from a Cultivar and a Commercial Hybrid of Wheat (Triticum aestivum L.)

Cited by 16 publications

References 6 publications

Regeneration potential of CIMMYT durum wheat and triticale varieties from immature embryos

Regeneration potential of CIMMYT durum wheat and triticale varieties from immature embryos

Effects of Cultivar and Explant Sources on Callus Induction and Plant Regeneration in Rice (Oryza sativa L.)

Somatic Embryogenesis on Plumule and Radicle Explants Obtained from Warm Water Hydroprimed Wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds

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