Effect of atropine on rat pancreatic secretory response to trypsin inhibitors and protein

Levan, Van H.; Green, G. M.

doi:10.1152/ajpgi.1986.251.1.g64

Cited by 14 publications

(11 citation statements)

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“…MK-329 abolished and atropine had no significant effect on protein or fluid secretion stimulated by 62.5 pmol⅐kg Ϫ1 ⅐h Ϫ1 of CCK-58 (not shown). The absence of inhibitory effects of atropine on pancreatic secretion stimulated by exogenous or endogenous cholecystokinin in conscious, recovered rats is well established (6,33,41). Surprisingly, MK-329 had no effect on the pancreatic fluid secretion caused by 500 pmol⅐kg Ϫ1 ⅐h Ϫ1 of CCK-8, while abolishing protein output (not shown).…”

Section: Role Of Cck-a Receptor Cholinergic Pathways and Secretin Imentioning

confidence: 80%

Water and enzyme secretion are tightly coupled in pancreatic secretion stimulated by food or CCK-58 but not by CCK-8

Yamamoto¹,

Reeve²,

Keire³

et al. 2005

American Journal of Physiology-Gastrointestinal and Liver Physi

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Pancreatic secretion of protein, water, chloride, and bicarbonate under basal conditions and in response to intravenous and intraduodenal stimuli were studied in awake rats fully recovered from surgery. During the basal phase of pancreatic secretion, protein output and water output were weakly correlated or uncorrelated, consistent with separate regulation and distinct cellular origin of enzyme (acinar cells) and water (duct cells), referred to as the two-component paradigm of pancreatic secretion. When pancreatic secretion was stimulated physiologically, water and protein output abruptly became strongly and significantly correlated, suggesting that protein secretion and water secretion are tightly coupled or that protein secretion is dependent on water secretion. The apparent function of this coupling is to resist or prevent increases in protein concentration as protein output increases. This pattern of secretion was reproduced by intravenous infusion of the CCK-58 form of cholecystokinin, which strongly stimulates pancreatic water and chloride secretion, but not by CCK-8, which only weakly stimulates water and chloride secretion in a non-dose-dependent manner. The remarkably tight association of water and protein secretion in food-stimulated and CCK-58-stimulated pancreatic secretion is consistent with a single cell type as the origin of both water and enzyme secretion, i.e., the acinar cell, and is not consistent with the two-component paradigm of pancreatic secretion. Because CCK-58 is the only detectable endocrine form of cholecystokinin in the rat and its bioactivity pattern is markedly and qualitatively different from CCK-8, actions previously recorded for CCK-8 should be reexamined.

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Section: Role Of Cck-a Receptor Cholinergic Pathways and Secretin Imentioning

confidence: 80%

Water and enzyme secretion are tightly coupled in pancreatic secretion stimulated by food or CCK-58 but not by CCK-8

Yamamoto¹,

Reeve²,

Keire³

et al. 2005

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…The effect of atropine on feedback regulation of pancreatic secretion however is controversial. Other investigators showed that atropine has no effect in conscious rats (28). Experimental design may account for these differences.…”

Section: Discussionmentioning

confidence: 96%

Peptone stimulates CCK-releasing peptide secretion by activating intestinal submucosal cholinergic neurons.

Li¹,

Owyang²

1996

J. Clin. Invest.

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In this study we tested the hypothesis that peptone in the intestine stimulates the secretion of the CCK-releasing peptide (CCK-RP) which mediates CCK secretion, and examined the enteric neural circuitry responsible for CCK-RP secretion. We used a "donor-recipient" rat intestinal perfusion model to quantify the CCK-RP secreted in response to nutrient stimulation. Infusion of concentrated intestinal perfusate collected from donor rat perfused with 5% peptone caused a 62 Ϯ 10% increase in protein secretion and an elevation of plasma CCK levels to 6.9 Ϯ 1.8 pM in the recipient rat. The stimulatory effect of the intestinal washings was abolished when the donor rats were pretreated with atropine or hexamethonium but not with guanethidine or vagotomy. Mucosal application of lidocaine but not serosal application of benzalkonium chloride which ablates the myenteric neurons in the donor rats also abolished the stimulatory action of the intestinal washings. Furthermore, treatment of the donor rats with a 5HT 3 antagonist and a substance P antagonist also prevented the secretion of CCK-RP. These observations suggest that peptone in the duodenum stimulates serotonin release which activates the sensory substance P neurons in the submucous plexus. Signals are then transmitted to cholinergic interneurons and to epithelial CCK-RP containing cells via cholinergic secretomotor neurons. This enteric neural circuitry which is responsible for the secretion of CCK-RP may in turn play an important role in the postprandial release of CCK. (

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“…The vagus nervous system has been assumed to be another factor that regulates pancreatic secretion, and the inhibition of the cholinergic nerve system reduces pancreatic secretion (7). It has been reported that the quantity of atropine used in this experiment is sufficient to inhibit pancreatic secretion (16). Despite the intravenous administration of this quantity of atropine, however, platycodin D started to stimulate pancreatic secretion 30 mm after administration (Fig.…”

Section: Discussionmentioning

confidence: 63%

Stimulative Effects of Saponin from Kikyo-to, a Japanese Herbal Medicine, on Pancreatic Exocrine Secretion of Conscious Rats

Arai¹,

Komatsu²,

Hirai³

et al. 1997

Planta Med

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Our previous report stated that kikyo-to, a Japanese herbal medicine, consisting of the roots of Platycodon grandiflorum and Glycyrrhiza sp., stimulates the pancreatic exocrine secretion of conscious rats. The present study focused on the effective components of kikyo-to and the mechanism of stimuli to pancreatic secretion of rats. When 10 to 100 mg of platycodin D, a saponin from the root of Platycodon grandiflorum, was intragastrically administered, the pancreatic secretion of rats was stimulated. At the same time, the plasma CCK concentration increased. On the other hand, the stimulative effects of glycyrrhizin, a saponin from the root of Glycyrrhiza sp. were weak compared to platycodin D. The effects of 10 mg/kg of platycodin D on pancreatic secretion were inhibited by loxiglumide (50 mg/kg, i.g.), a CCK receptor antagonist. In contrast, the suppressive effect of atropine (300 micrograms/kg/h, i.v.) on pancreatic secretion was reduced by administering 10 mg/kg of platycodin D. In addition, up to 1 mM of platycodin D did not inhibit the trypsin activities in vitro. In conclusion, kikyo-to serves to stimulate pancreatic exocrine secretion mainly because platycodin D causes gastrointestinal hormones, particularly, CCK to be released from the duodenum.

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Effect of atropine on rat pancreatic secretory response to trypsin inhibitors and protein

Cited by 14 publications

References 0 publications

Water and enzyme secretion are tightly coupled in pancreatic secretion stimulated by food or CCK-58 but not by CCK-8

Water and enzyme secretion are tightly coupled in pancreatic secretion stimulated by food or CCK-58 but not by CCK-8

Peptone stimulates CCK-releasing peptide secretion by activating intestinal submucosal cholinergic neurons.

Stimulative Effects of Saponin from Kikyo-to, a Japanese Herbal Medicine, on Pancreatic Exocrine Secretion of Conscious Rats

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