Effect of ASTRA-BEN 20® on Broiler Chicks Exposed to Aflatoxin B1 or T2 Toxin

Fairchild, A. S.; Croom, J.; Grimes, J. L.; Hagler, W. M.

doi:10.3923/ijps.2008.1147.1151

Cited by 7 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No pH effect was observed across any of the treatments, suggesting that AB20A sequestered the toxins when the peanut protein had a net positive (pH 2.0) or a net negative (pH 8.0) charge. Likewise, 2% addition of an AB20A analogue adsorbed at least 97% of AfB 1 from 10% methanol solutions adjusted to pH 3.0, 7.0, or 10.1 (17). Taken together, these studies suggest that hydrophobic interactions predominate over electrostatic effects in the binding mechanism of this clay.…”

Section: Resultsmentioning

confidence: 66%

Value-Added Processing of Peanut Meal: Aflatoxin Sequestration during Protein Extraction

Seifert

Davis

Dorner

et al. 2010

J. Agric. Food Chem.

View full text Add to dashboard Cite

The efficacy of a bentonite clay, Astra-Ben 20A (AB20A), to sequester aflatoxin from contaminated (approximately 110 ppb) peanut meal during protein extraction was studied. Aqueous peanut meal dispersions (10% w/w) were prepared by varying the pH, temperature, enzymatic hydrolysis conditions, and concentrations of AB20A. After extraction, dispersions were centrifuged and filtered to separate both the water-soluble and the water-insoluble fractions for subsequent testing. Inclusion of AB20A at 0.2 and 2% reduced (p < 0.05) aflatoxin concentrations below 20 ppb in both fractions; however, the higher concentration of AB20A also reduced (p < 0.05) the water-soluble protein content. Inclusion of 0.2% AB20A did not affect protein solubility, total soluble solids, or degree of hydrolysis. Peanut meal adsorption isotherms measured the AB20A capacity to sequester aflatoxin. These results are discussed in the context of a process designed to sequester aflatoxin from contaminated peanut meal, which could enable derivatives of this high protein material to be utilized in enhanced feed and/or food applications.

show abstract

Section: Resultsmentioning

confidence: 66%

Value-Added Processing of Peanut Meal: Aflatoxin Sequestration during Protein Extraction

Seifert

Davis

Dorner

et al. 2010

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…The decreased toxicity of aflatoxins observed for contaminated animal feed mixed with clay feed additives has stimulated research on clay additives to prevent mycotoxicosis. Animal feeding studies have demonstrated that the clay additives, Novasil plus, Astra Ben 20A, Na-bentonite, zeolite, and sepiolite can effectively reduce or prevent toxicity caused by feed contaminated with Aspergillus mycotoxins, such as AfB1 [ 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. Smectite interlayer expansion of >1.2 nm stable up to 400 °C after AfB1 treatment demonstrated that AfB1 adsorbs to interlayer clay surfaces [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Aflatoxin Toxicity Reduction in Feed by Enhanced Binding to Surface-Modified Clay Additives

Jaynes

Zartman

2011

Toxins

View full text Add to dashboard Cite

Animal feeding studies have demonstrated that clay additives, such as bentonites, can bind aflatoxins in ingested feed and reduce or eliminate the toxicity. Bentonite deposits are found throughout the world and mostly consist of expandable smectite minerals, such as montmorillonite. The surfaces of smectite minerals can be treated with organic compounds to create surface-modified clays that more readily bind some contaminants than the untreated clay. Montmorillonites treated with organic cations, such as hexadecyltrimethylammonium (HDTMA) and phenyltrimethylammonium (PTMA), more effectively remove organic contaminants, such as benzene and toluene, from water than untreated clay. Similarly, montmorillonite treated with PTMA (Kd = 24,100) retained more aflatoxin B1 (AfB1) from aqueous corn flour than untreated montmorillonite (Kd = 944). Feed additives that reduced aflatoxin toxicity in animal feeding studies adsorbed more AfB1 from aqueous corn flour than feed additives that were less effective. The organic cations HDTMA and PTMA are considered toxic and would not be suitable for clay additives used in feed or food, but other non-toxic or nutrient compounds can be used to prepare surface-modified clays. Montmorillonite (SWy) treated with choline (Kd = 13,800) and carnitine (Kd = 3960) adsorbed much more AfB1 from aqueous corn flour than the untreated clay (Kd = 944). A choline-treated clay prepared from a reduced-charge, high-charge montmorillonite (Kd = 20,100) adsorbed more AfB1 than the choline-treated high-charge montmorillonite (Kd = 1340) or the untreated montmorillonite (Kd = 293). Surface-modified clay additives prepared using low-charge smectites and nutrient or non-toxic organic compounds might be used to more effectively bind aflatoxins in contaminated feed or food and prevent toxicity.

show abstract

“…• Bailey et al (2006) incorporated MNT clay (0.5%) into AF-CT (4 ppm) broiler; they reported that MNT clay in broiler diets provided significant protection on growth performance, serum biochemistry, and relative organ weight associated with aflatoxicosis. • Fairchild et al (2008) added bentonite based Astra-Ben (1 and 2%) to AF-CT (4 ppm) broiler diet; the adsorbent provided significant improvements in performance and liver lipid content.…”

Section: United Statesmentioning

confidence: 99%