Effect of Aspartate and Asparagine Supplementation on Fatigue Determinants in Intense Exercise

Marquezi, Marcelo Luís; Roschel, Hamilton; Costa, André dos Santos; Sawada, L A; Lancha, Antônio Herbert

doi:10.1123/ijsnem.13.1.65

Cited by 70 publications

(51 citation statements)

References 20 publications

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“…Some amino acids, such as glutamic acid, have antioxidant activity and anti-exercise-fatigue effect (Guezennec et al 1998;Niranjan et al 2005). Other amino acids, such as aspartic acid, have anti-exercise-fatigue effect without antioxidant activity (Marquezi et al 2003). This result indicated that the antioxidant activity and anti-exercise-fatigue effect of highly denatured soybean meal hydrolysate might result from different amino acids.…”

Section: Amino Acid Compositionmentioning

confidence: 79%

“…Guezennec et al (1998) reported that glutamic acid had a very positive effect on the nervous system and would also be helpful during exercise. Marquezi et al (2003) reported that aspartic acid was helpful in the oxidative deamination and could lower the blood ammonia concentration, therefore delaying the occurrence of fatigue. The above two amino acids composition, group B, C, and D were 27.26 %, 27.34 %, and 28.38 % respectively.…”

Section: Amino Acid Compositionmentioning

confidence: 99%

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Antioxidant activity and anti-exercise-fatigue effect of highly denatured soybean meal hydrolysate prepared using neutrase

2013

J Food Sci Technol

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Highly denatured soybean meal is a by-product of soybean oil extraction obtained through high-temperature desolventization. High-temperature treatment can result in soybean protein denaturation. Compare with ordinary soybean meal, the protein structure of highly denatured soybean meal has changed. Highly denatured soybean meal was pretreated with thermal treatment or ultrasonication, and then hydrolyzed with neutrase. The ultrasonicated hydrolysate exhibited better antioxidant activity than the thermally treated hydrolysate. The ultrasonication increased 1,1-diphenyl-2-pycryl hydrazyl (DPPH) radical scavenging activity by 8.31 % and reduction capacity by 10.19 %. The highly denatured soybean meal hydrolysate ultrasonicated at 400 W exhibited the highest antioxidant activity. The DPPH radical scavenging activity was 56.22 % and reduction capacity was 0.717. The ultrasonicated hydrolysate at 400 W was fractionated using ultrafiltration into three fractions: I (>10 kDa), II (5 kDa to 10 kDa), and III (<5 kDa). The in vitro antioxidant activity and others in vivo anti-exercise-fatigue effect of the three fractions (I, II, and III) were determined. Fraction III exhibited the highest DPPH radical scavenging activity and reduction capacity, improved the hemoglobin and hepatic glycogen content and reduced blood urea nitrogen and blood lactic acid. Fraction III improved the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) and reduced the malonaldehyde (MDA) content in mouse livers. Therefore, the highly denatured soybean meal hydrolysate has an anti-oxidative effect and it significantly alleviates exercisefatigue in mice. Amino acids of hydrolysate were determined.Results showed that the antioxidant activity and anti-exercisefatigue effect were related to the amino acid compositions.

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Section: Amino Acid Compositionmentioning

confidence: 79%

Section: Amino Acid Compositionmentioning

confidence: 99%

Antioxidant activity and anti-exercise-fatigue effect of highly denatured soybean meal hydrolysate prepared using neutrase

2013

J Food Sci Technol

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“…2 We have demonstrated previously that Aspartate (Asp) and Asparagine (Asn) supplementation in the drinking water of endurance-trained rats increases their muscle glycogen concentration, and delays fatigue. 3 Furthermore, Marquezi and co-authors 4 reported that Asp and Asn supplementation decreases the plasma lactate concentration in rats and increases the time to fatigue during high intensity exercise. In the available literature, the impact of aspartate on endurance seems generally favorable in humans.…”

Section: Introductionmentioning

confidence: 98%

The effect of 5 days of aspartate and asparagine supplementation on glucose transport activity in rat muscle

Lancha

Poortmans²,

Pereira

2009

Cell Biochemistry & Function

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The consumption of protein supplements containing amino acids is increasing around the world. Aspartate (Asp) and asparagine (Asn) are amino acids metabolized by skeletal muscle. This metabolism involves biochemical pathways that are involved in increasing Krebs cycle activity via anaplerotic reactions, resulting in higher glutamine concentrations. A connection between amino acid supplementation, glycogen concentration, and glucose uptake has been previously demonstrated. The purpose of this study was to evaluate the effect of Asp and Asn supplementation on glucose uptake in rats using three different glycogen concentrations. The results indicate that Asp and Asn supplementation in rats with high glycogen concentrations (fed state) further increased the glycogen concentration in the muscle, and decreased in vitro 2-deoxyglucose (a glucose analog) uptake by the muscle at maximal insulin concentrations. When animals had a medium glycogen concentration (consumed lard for 3 days), glucose uptake was higher in the supplemented group at sub-maximal insulin concentrations. We conclude that supplementation of Asp and Asn reduced glucose transport in rat muscle only at higher levels of glycogen. The ingestion of lard for 3 days changed the responsiveness and sensitivity to insulin, and that group had higher levels of insulin sensitivity with Asp and Asn supplementation.

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“…In addition, red mold rice contains gamma-aminobutyric acid and glutamic acid, which have positive effects on the nervous system and could help in executing exercise (Guezennec et al, 1998). Marquezi et al (2003) indicated that aspartic acid is helpful in oxidative deamination and can lower the blood ammonia concentration, thus delaying fatigue.…”

Section: Anti-fatigue Activitymentioning

confidence: 99%

“…Soybean peptides are rich in amino acids such as leucine (23.6%), valine (12.22%), lysine (10.01%), isoleucine (8.8%), and phenlyalanine (8.75%). In addition, soybean peptides contain 4.74% aspartate and 5.55% glutamate, which help in performing exercise (Guezennec et al, 1998) and delaying fatigue (Marquezi et al, 2003). A supply of amino acids, especially branched-chain amino acids, can improve exercise ability and markedly retard the catabolism of proteins in muscle during exercise (Blomstrand & Newsholme, 1992).…”

Section: Anti-fatigue Activitymentioning

confidence: 99%

Beneficial Effects of Bioactive Peptides Derived from Soybean on Human Health and Their Production by Genetic Engineering

Liu¹,

Pan²

2011

Soybean and Health

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Effect of Aspartate and Asparagine Supplementation on Fatigue Determinants in Intense Exercise

Abstract: These results suggest that ASP+ASG supplementation may increase the contribution of oxidative metabolism in energy production and delay fatigue during exercise performed above the AT.

Cited by 70 publications

References 20 publications

Antioxidant activity and anti-exercise-fatigue effect of highly denatured soybean meal hydrolysate prepared using neutrase

Antioxidant activity and anti-exercise-fatigue effect of highly denatured soybean meal hydrolysate prepared using neutrase

The effect of 5 days of aspartate and asparagine supplementation on glucose transport activity in rat muscle

Beneficial Effects of Bioactive Peptides Derived from Soybean on Human Health and Their Production by Genetic Engineering

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