The effect of 5 days of aspartate and asparagine supplementation on glucose transport activity in rat muscle

Lancha, Antônio Herbert; Poortmans, Jacques; Pereira, Luiza de Oliveira Ramos

doi:10.1002/cbf.1606

Cited by 14 publications

(13 citation statements)

References 26 publications

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“…Mice were divided into three groups: (a) untreated KK mice (Control group; n = 5), (b) untreated KK‐Akita mice (DKD group; n = 5), and (c) treated (fed a specified diet, i.e., CE‐2 with doubled Asp) KK‐Akita mice (Tx group; n = 5). As shown in Table 1, we prepared a specified diet with a double volume of not only Asp but also asparagine, the precursor of Asp, as reported previously [17]. All groups were weaned by 4 weeks of age and fed CE‐2 until 6 weeks of age.…”

Section: Methodsmentioning

confidence: 99%

Aspartic acid supplementation ameliorates symptoms of diabetic kidney disease in mice

et al. 2020

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Diabetic kidney disease (DKD) is among the most common and serious complications of both type 1 and type 2 diabetes. In this study, we used KK/Ta‐Ins2Akita (KK‐Akita) mice as a model of DKD and KK/Ta (KK) mice as controls to identify novel factors related to the development/progression of DKD. Capillary electrophoresis coupled with mass spectrometry analysis revealed that circulating Asp (l‐aspartic acid) levels in diabetic KK‐Akita mice tend to be lower than those in control KK mice. Therefore, we evaluated the effect of Asp supplementation to prevent the progression of DKD in KK‐Akita mice. Mice were divided into three groups: (a) untreated KK mice (Control group), (b) untreated KK‐Akita mice (DKD group), and (c) treated (double‐volume Asp diet) KK‐Akita mice (Tx group). Kidney sections were stained with fluorescein isothiocyanate‐labeled lectins, wheat germ agglutinin (WGA), and anti‐endothelial nitric oxide synthase (eNOS) antibody for evaluation of endothelial surface layer (ESL) and NO synthesis. The mesangial area and glomerular size in the DKD group were significantly larger than those in the Control group; however, there was no significant difference in those between the DKD and Tx groups. Albuminuria, the ratio of foot process effacement, and thickness of glomerular basement membrane in the Tx group were significantly lower than those in the DKD group. Furthermore, the expression levels of glomerular WGA and microvascular eNOS in the Tx group improved significantly and approached the level in the Control group. In conclusion, the improvement of albuminuria in the Tx group may be caused by the reduction of oxidative stress in the kidneys, which may lead to the subsequent improvement of glomerular ESL.

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Section: Methodsmentioning

confidence: 99%

Aspartic acid supplementation ameliorates symptoms of diabetic kidney disease in mice

et al. 2020

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“…This would lead to a negative feedback of muscle and liver glycogen upon glycogen synthase activity and, consequently, on glycogen storage. Recently, Lancha Jr et al85 demonstrated the inverse relationship between glycogen content and glucose uptake, ie, the fed animals featured higher glycogen storage and lower glucose uptake whereas the fasted animals had the opposite situation. However, currently, the mechanisms related to glycogen and glucose transport are uncertain; although, some authors believe that glycogen could regulate glucose transport by agglutination of glucose transporter type 4 (GLUT4) molecules and further incorporation into glycogen complex and enzymes.…”

Section: Obesity-inducing Factorsmentioning

confidence: 99%

Obesity: considerations about etiology, metabolism, and the use of experimental models

Pereira-Lancha¹,

Campos-Ferraz²,

Lancha³

2012

DMSO

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Studies have been conducted in order to identify the main factors that contribute to the development of obesity. The role of genetics has also been extensively studied. However, the substantial augmentation of obesity prevalence in the last 20 years cannot be justified only by genetic alterations that, theoretically, would have occurred in such a short time. Thus, the difference in obesity prevalence in various population groups is also related to environmental factors, especially diet and the reduction of physical activity. These aspects, interacting or not with genetic factors, could explain the excess of body fat in large proportions worldwide. This article will focus on positive energy balance, high-fat diet, alteration in appetite control hormones, insulin resistance, amino acids metabolism, and the limitation of the experimental models to address this complex issue.

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“…10 In addition, Asp can be metabolized by skeletal muscle, and its combinations with other substances play an important role on muscle metabolism. 11,12 For example, Lancha et al 11 reported that Asp and asparagine supplementation increased glycogen concentration and modulated the glucose uptake in rat muscle. Lancha et al 12 also reported that Asp, asparagine, and carnitine supplementation increased the capacity of muscle to utilize free fatty acids and spare glycogen.…”

Section: Introductionmentioning

confidence: 99%

Aspartate inhibits LPS-induced MAFbx and MuRF1 expression in skeletal muscle in weaned pigs by regulating Akt, AMPKα and FOXO1

et al. 2016

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Infection and inflammation can result in the rapid loss of muscle mass and myofibrillar proteins (muscle atrophy). In addition, aspartate (Asp) is necessary for protein synthesis in mammalian cells. We hypothesized that Asp could attenuate LPS-induced muscle atrophy in a piglet model. Twenty-four weaning piglets were allotted to four treatments, including non-challenged control, LPS challenged control, LPS+0.5% Asp and LPS+1.0% Asp. On d 21, the piglets were injected with i.p. injection of LPS (100 ug/kg BM) or saline. At 4 h post-injection, blood, gastrocnemius and longissimus dorsi muscles samples were collected for biochemical and molecular analyses. Asp decreased the concentrations of cortisol and glucagon in plasma. In addition, Asp increased protein and RNA contents in muscles, and decreased mRNA expression of muscle atrophy F-box (MAFbx) and muscle RING finger 1 (MuRF1). Moreover, Asp decreased phosphorylation of AMPKα but increased phosphorylation of Akt and Forkhead Box O (FOXO) 1 in the muscles. Our results indicate that Asp suppresses LPS-induced MAFbx and MuRF1 expression via activation of Akt signaling, and inhibition of AMPKα and FOXO1 signaling.

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The effect of 5 days of aspartate and asparagine supplementation on glucose transport activity in rat muscle

Cited by 14 publications

References 26 publications

Aspartic acid supplementation ameliorates symptoms of diabetic kidney disease in mice

Aspartic acid supplementation ameliorates symptoms of diabetic kidney disease in mice

Obesity: considerations about etiology, metabolism, and the use of experimental models

Aspartate inhibits LPS-induced MAFbx and MuRF1 expression in skeletal muscle in weaned pigs by regulating Akt, AMPKα and FOXO1

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