Effect of aromatase inhibitors on ectopic endometrial growth and peritoneal environment in a mouse model of endometriosis

Bilotas, Mariela Andrea; Meresman, Gabriela Fabiana; Stella, Inés; Sueldo, Carlos; Barañao, Rosa Inés

doi:10.1016/j.fertnstert.2009.08.058

Cited by 54 publications

(50 citation statements)

References 45 publications

(62 reference statements)

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“…In this sense, investigations are focusing on finding new and more effective alternatives for patients. In this study, and taking into account previous results obtained in our laboratory (Bilotas et al 2010 and earlier promising results obtained in cancer (Chow et al 2008, Falandry et al 2009), we decided to combine two inhibitors: COX-2 and aromatase. Already some years ago, Ebert et al (2005) reviewed the importance of aiming at these molecules given the abnormalities present within the eutopic and ectopic endometrium of endometriosis patients and acknowledging the positive feedback loop present between these molecules .…”

Section: Aromatase and Cox-2 Inhibitors In Endometriosismentioning

confidence: 99%

“…This is an approach and a modelization of a very complicated human disease and the results should be interpreted in this sense. Nevertheless, this is a well-established and accepted model of endometriosis, which has been thoroughly used not only in our laboratory (Bilotas et al 2010 but by other investigators too , Becker et al 2006, Grummer 2006, Pelch et al 2010.…”

Section: Aromatase and Cox-2 Inhibitors In Endometriosismentioning

confidence: 99%

“…Endometriosis-like lesions were induced through transplantation of one of the uterine horns to the bowel mesentery as described previously (Bilotas et al 2010. Briefly, animals were deeply anesthetized with an i.p.…”

Section: Surgical Induction Of Endometriosis and Treatmentmentioning

confidence: 99%

“…In this sense, two aromatase inhibitors, letrozole and anastrozole, have also been tested in vitro and in vivo in our laboratory and demonstrated to have antiproliferative and proapoptotic effects (Meresman et al 2005, Bilotas et al 2010.…”

Section: Introductionmentioning

confidence: 99%

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Anastrozole and celecoxib for endometriosis treatment, good to keep them apart?

et al. 2013

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Endometriosis is a benign gynecological disease. Cyclooxygenase-2 (COX-2) and aromatase proteins have been shown to be overexpressed in eutopic endometrium from women suffering from this disease compared to disease-free women. Furthermore, inhibition of these molecules individually was demonstrated to have antiproliferative and proapoptotic effects both in vitro and in vivo in several models. In this study, the effect of combining celecoxib, a selective COX-2 inhibitor, and anastrozole, an aromatase inhibitor, on the implantation and growth of endometriotic like lesions in a murine model of endometriosis was evaluated. Endometriosis was surgically induced in female BALB/c mice. After 28 days of treatment with celecoxib, anastrozole, or their combination, animals were killed and lesions were counted, measured, excised, and fixed. Immunohistochemistry for proliferating cell nuclear antigen and CD34 was performed for assessment of cell proliferation and vascularization. TUNEL technique was performed for apoptosis evaluation. Celecoxib was the only treatment to significantly reduce the number of lesions established per mouse, their size and vascularized area. In addition, cell proliferation was significantly diminished and apoptosis was significantly enhanced by both individual treatments. When the therapies were combined, they reversed their effects. These results confirm that celecoxib and anastrozole separately decrease endometriotic growth, but when combined they might have antagonizing effects.

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Section: Aromatase and Cox-2 Inhibitors In Endometriosismentioning

confidence: 99%

Section: Aromatase and Cox-2 Inhibitors In Endometriosismentioning

confidence: 99%

Section: Surgical Induction Of Endometriosis and Treatmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anastrozole and celecoxib for endometriosis treatment, good to keep them apart?

et al. 2013

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“…When letrozole or anastrozole were added to endometrial epithelial cells from endometriosis patients in culture, cell proliferation was inhibited and apoptosis augmented (Meresman et al, 2005). When the same two compounds were used for the treatment of surgically induced endometriosis in mice, not only cell proliferation was diminished and apoptosis increased within the endometriotic like lesion, but PGE was also decreased in the peritoneal fluid of mice treated with letrozole but not with anastrozole (Bilotas et al, 2010).…”

Section: Pge 2 Synthesis Inhibition and The Control Over Endometriosimentioning

confidence: 99%