Effect of arginine on the growth and biofilm formation of oral bacteria

Huang, Xuelian; Zhang, Ke-Ke; Deng, Meng; Exterkate, R.A.M.; Liu, Chengcheng; Zhou, Xuedong; Cheng, Lei; Cate, J.M. ten

doi:10.1016/j.archoralbio.2017.06.026

Cited by 88 publications

(61 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, the results of a previous study revealed the potential role of the antibacterial effect on P. gingivalis [25]. On the basis of these extended preliminary experiments, d-arginine was selected as the target amino acid, considering that the arginine residue may have a potential role in antimicrobial property [40]. Arginine residue participates in the composition of Arg-gingipain of P. gingivalis, and this was explored using its chiral isomer.…”

Section: Discussionmentioning

confidence: 99%

Effects of D-arginine on <i>Porphyromonas gingivalis</i> biofilm

Liu

et al. 2020

J Oral Sci

View full text Add to dashboard Cite

Porphyromonas gingivalis (P. gingivalis) is one of the major pathogenic bacteria of periodontitis or peri-implantitis. P. gingivalis tends to attach to the implant's neck with the formation of biofilm, leading to peri-implantitis. d-arginine has been shown to have a potential antimicrobial role. In this study, P. gingivalis was cultured in Brain Heart Infusion broth together with d-arginine. After 3 days (inhibition) or 6 days (dissociation), these were characterized using crystal violet (CV) staining for the biofilm, extracellular polysaccharide (EPS) production from the biofilm, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay for biofilm activation. Furthermore, the P. gingivalis biofilm was observed by scanning electron microscopy (SEM). d-arginine effectively reduced biomass accumulation and promoted dissociation at concentrations of ≥50 mM and 100 mM, respectively. Through CV staining, d-arginine concentrations of EPS production from the biofilm for inhibition and dissociation effects was ≥50 mM and 100 mM, respectively. In addition, d-arginine affected biofilm activation for the corresponding concentrations: ≥60 mM for inhibition and ≥90 mM for dispersal. Under SEM observation, d-arginine changed the P. gingivalis biofilm structure in relatively high concentrations for inhibition or dissociation, respectively. The authors concluded that d-arginine could inhibit the formation of P. gingivalis biofilm and promote the dissociation of P. gingivalis biofilm.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of D-arginine on <i>Porphyromonas gingivalis</i> biofilm

Liu

et al. 2020

J Oral Sci

View full text Add to dashboard Cite

show abstract

“…Ryan et al [34] found that arginine deiminase (ADI) genes can help Listeria monocytogenes survive under acidic conditions and that their expression is increased at low pH and in the presence of arginine. Huang et al [35] found that l-arginine can inhibit the biofilm formation of Streptococcus mutants and that although no direct evidence has indicated a role of biofilms in acid tolerance, the cell wall/ membrane is essential for maintaining cellular integrity. What's more, ZMO_RS04405 encodes an ABC transporter substrate-binding protein was reported to be critical for cells against furfural stress.…”

Section: Table 1 Conversion Of Glucose To Ethanol In Z Mobilis Undermentioning

confidence: 99%

Genome shuffling enhances stress tolerance of Zymomonas mobilis to two inhibitors

Wang

Han

et al. 2019

Biotechnol Biofuels

View full text Add to dashboard Cite

Background: Furfural and acetic acid are the two major inhibitors generated during lignocellulose pretreatment and hydrolysis, would severely inhibit the cell growth, metabolism, and ethanol fermentation efficiency of Zymomonas mobilis. Effective genome shuffling mediated by protoplast electrofusion was developed and then applied to Z. mobilis. Results: After two rounds of genome shuffling, 10 different mutants with improved cell growth and ethanol yield in the presence of 5.0 g/L acetic acid and 3.0 g/L furfural were obtained. The two most prominent genome-shuffled strains, 532 and 533, were further investigated along with parental strains in the presence of 7.0 g/L acetic acid and 3.0 g/L furfural. The results showed that mutants 532 and 533 were superior to the parental strain AQ8-1 in the presence of 7.0 g/L acetic acid, with a shorter fermentation time (30 h) and higher productivity than AQ8-1. Mutant 533 exhibited subtle differences from parental strain F34 in the presence of 3.0 g/L furfural. Mutations present in 10 genome-shuffled strains were identified via whole-genome resequencing, and the source of each mutation was identified as either de novo mutation or recombination of the parent genes. Conclusions: These results indicate that genome shuffling is an efficient method for enhancing stress tolerance in Z. mobilis. The engineered strains generated in this study could be potential cellulosic ethanol producers in the future.

show abstract

“…GtfC (encoded by gtfC), catalyse the synthesis of water insoluble and alkali soluble glucan from sucrose with both α-1,3 and α-1,6-linked glucans, which are required for biofilm formation and structurally stable biofilms. The gtfC Mutant strains of S. mutans is less cariogenic than the wild type strains in vivo [37,38] Therefore, the reduction in the expression of tested genes will thereby suppress a series of various cariogenic virulence factors in S. mutans.…”

Section: Gene Expressionmentioning

confidence: 99%

Effects of resveratrol on cariogenic virulence properties of Streptococcus mutans

Zhu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Streptococcus mutans is the principal etiological agent of human dental caries. The major virulence factors of S. mutans are acid production, acid tolerance, extracellular polysaccharide (EPS) synthesis and biofilm formation. The aim of this study is to evaluate the effect of resveratrol, a natural compound, on virulence properties of S. mutans . Methods: The effect of resveratrol on acidogenicity was investigated by pH drop and lactate dehydrogenase (LDH) assay, on aciduricity through survival rate at pH 5.0 and F-ATPase assay, on EPS synthesis (including water-soluble polysaccharide and water-insoluble polysaccharide) by quantitative measurement, on biofilm formation by crystal violet assay and structure observation. The expression of related virulence genes was also detected . Results: Resveratrol at sub-MIC levels can significantly decrease acid production, acid tolerance, EPS synthesis and biofilm formation. Related virulence gene expression ( ldh , relA , gtfC , comDE ) was down-regulated with increasing concentrations of resveratrol. Conclusion: Resveratrol has an inhibitory effect on S. mutans cariogenic virulence properties and it represents a promising anticariogenic agent .

show abstract

Effect of arginine on the growth and biofilm formation of oral bacteria

Cited by 88 publications

References 43 publications

Effects of D-arginine on <i>Porphyromonas gingivalis</i> biofilm

Effects of D-arginine on <i>Porphyromonas gingivalis</i> biofilm

Genome shuffling enhances stress tolerance of Zymomonas mobilis to two inhibitors

Effects of resveratrol on cariogenic virulence properties of Streptococcus mutans

Contact Info

Product

Resources

About