Effect of aqueous extract of Tribulus terrestris on oxalate-induced oxidative stress in rats

Abstract

The present study was aimed at studying the effect of Tribulus terrestris on different parameters of oxidative stress and gene expression profiles of antioxidant enzymes in renal tissues of male wistar rats after induction of hyperoxaluria. The animals were divided into three groups. The animals in group I (control) were administered vehicle only. In group II, the animals were treated with ethylene glycol (hyperoxaluric agent) and those in group III were administered T. terrestris plant extract in addition to ethylene glycol. All treatments were continued for a period of seven weeks. Ethylene glycol feeding resulted in hyperoxaluria as well as increased excretion of calcium and phosphate. Serum creatinine, uric acid and blood urea nitrogen levels were also altered in hyperoxaluric animals. Various oxidative stress parameters viz. lipid peroxidation and activity of antioxidant enzymes were used to confirm the peroxidant state. Reverse transcription-polymerase chain reaction (RT-PCR) analysis was used to confirm whether steady-state transcription level of different antioxidant enzymes was altered. T. terrestris significantly reduced the excretion of oxalate, calcium, and phosphate along with decreased levels of blood urea nitrogen, uric acid and creatinine in serum. T. terrestris also reduced hyperoxaluria- caused oxidative stress, and restored antioxidant enzyme activity and their expression profile in kidney tissue. Histological analysis depicted that T. terrestris treatment decreased renal epithelial damage, inflammation, and restored normal glomerular morphology.

Assessment of Protective and Antioxidant Properties of Tribulus Terrestris Fruits against Testicular Toxicity in Rats

“…In addition, the reported anti-oxidant activity of METT in this study was evident from the enhancement of activities of testicular anti-oxidant enzymes. The anti-oxidant effect of T. terrestris extract was similar to that previously demonstrated [ 32 , 33 ]. The anti-oxidant activity of T. terrestris fruits was attributed to the presence of active derivatives of 4,5-di-p-coumaroylquinic acid, which were isolated from the fruits and reported to have potent anti-oxidant activity [ 34 ].…”

Comparative evaluation of the sexual functions and NF-κB and Nrf2 pathways of some aphrodisiac herbal extracts in male rats

“…Tribulus terrestris were shown to increase the nitric oxide release and this is considered for its aphrodisiac capacity character [48, 49]. Our results suggested major increases in Nrf-2 and HO-1 levels and decreases in NF-κB and MDA levels of the various reproductive tissue parts and serum when administered with Tribulus terrestris similarly to a study which the extract was restored antioxidant enzyme activity and their expression profile in kidney tissue [50] and another study that extract blocked proliferation and induced apoptosis in cancer cells through the inhibition of NF-κB signaling [51]. …”

Effects of the Fruit Extract of Tribulus terrestris on Skin Inflammation in Mice with Oxazolone‐Induced Atopic Dermatitis through Regulation of Calcium Channels, Orai‐1 and TRPV3, and Mast Cell Activation

“…Its target organ is the liver, and it is effective for the control of headaches, dizziness, breast lumps, chest pain, flank pain, intestinal pain, and problems with breast milk circulation. Modern pharmacological studies have detailed its various effects against diabetes [ 8 ] and liver and kidney toxicity [ 7 ] as well as oxidative damage [ 10 ], but its herbal features and clinical efficacy are not well known. Therefore, in this study, we investigated the efficacy of TF extract with or without 0.1% HC to improve AD using an Ox-induced AD mice compared to 1% HC application as a common topical dosage.…”

“…TF is also used in folk medicine for a tonic, aphrodisiac, palliative, astringent, stomachic, antihypertensive, diuretic, lithontriptic, and urinary anti-infective agents [ 5 ]. In modern experimental studies, TF has been reported to have protective effects against liver and kidney toxicity [ 6 – 9 ], antidiabetic effects [ 8 ], and antioxidant effects [ 10 ]. This plant contains known bioactive compounds, such as cytoprotective lignanamides [ 10 ], anthelminthic [ 11 ] and antifungal saponin [ 12 ], and anti-inflammatory N-trans- ρ -caffeoyl tyramine [ 13 ].…”