Effect of antiplaque compounds and mouthrinses on the activity of glucosyltransferases from <i>Streptococcus sobrinus</i> and insoluble glucan production

Furiga, Aurélie; Dols‐Lafargue, Marguerite; Heyraud, A.; Chambat, G.; Lonvaud‐Funel, Aline; Badet, Cécile

doi:10.1111/j.1399-302x.2008.00441.x

Cited by 20 publications

(13 citation statements)

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“…sobrinus GTF complex, and leading to the synthesis of a strongly adhesive insoluble glucan (Furiga et al . ). Thus, the inhibition observed by GSE of both the initial overall reaction rate and the IG synthesis by this GTF preparation is representative of the inhibition of the cooperative action of GTFs.…”

Section: Discussionmentioning

confidence: 97%

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Preventive effects of an original combination of grape seed polyphenols with amine fluoride on dental biofilm formation and oxidative damage by oral bacteria

2013

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Section: Discussionmentioning

confidence: 97%

“…Crude cell-free GTF preparation was obtained from a culture of Strep. sobrinus ATCC 33478 by ammonium sulfate precipitation as previously described (Furiga et al 2008a).…”

Section: Effect On Glucosyltransferases Activitymentioning

confidence: 99%

Preventive effects of an original combination of grape seed polyphenols with amine fluoride on dental biofilm formation and oxidative damage by oral bacteria

2013

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“…In addition, many natural agents have been explored for their effect on Gtf activity, such as hops, green tea, traditional medicinal plants and food extracts [Wu-Yuan et al, 1988; Ikeno et al, 1991; Otake et al, 1991; Nakahara et al, 1993; Wolinsky et al, 1996; Tagashira et al, 1997; Osawa et al, 2001; Steinberg et al, 2004; Furiga et al, 2008; Hannig et al, 2008b; Yaegaki et al, 2008]. Carbohydrate fatty esters had no effect on Gtf activity from S. sobrinus despite inhibiting growth of the organisms [Devulapalle et al, 2004].…”

Section: Inhibition Of Gtfsmentioning

confidence: 99%

Biology of <i>Streptococcus mutans-</i>Derived Glucosyltransferases: Role in Extracellular Matrix Formation of Cariogenic Biofilms

Bowen

Koo²

2011

Caries Res

872

1,016

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The importance of Streptococcus mutans in the etiology and pathogenesis of dental caries is certainly controversial, in part because excessive attention is paid to the numbers of S. mutans and acid production while the matrix within dental plaque has been neglected. S. mutans does not always dominate within plaque; many organisms are equally acidogenic and aciduric. It is also recognized that glucosyltransferases from S. mutans (Gtfs) play critical roles in the development of virulent dental plaque. Gtfs adsorb to enamel synthesizing glucans in situ, providing sites for avid colonization by microorganisms and an insoluble matrix for plaque. Gtfs also adsorb to surfaces of other oral microorganisms converting them to glucan producers. S. mutans expresses 3 genetically distinct Gtfs; each appears to play a different but overlapping role in the formation of virulent plaque. GtfC is adsorbed to enamel within pellicle whereas GtfB binds avidly to bacteria promoting tight cell clustering, and enhancing cohesion of plaque. GtfD forms a soluble, readily metabolizable polysaccharide and acts as a primer for GtfB. The behavior of soluble Gtfs does not mirror that observed with surface-adsorbed enzymes. Furthermore, the structure of polysaccharide matrix changes over time as a result of the action of mutanases and dextranases within plaque. Gtfs at distinct loci offer chemotherapeutic targets to prevent caries. Nevertheless, agents that inhibit Gtfs in solution frequently have a reduced or no effect on adsorbed enzymes. Clearly, conformational changes and reactions of Gtfs on surfaces are complex and modulate the pathogenesis of dental caries in situ, deserving further investigation.

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“…Properties of polyphenols include radical scavenging, involved in antioxidant reactions, and structural interactions with proteins [Cheynier, 2005]. Among these, polyphenolic compounds inhibit the glycosyltransferase activity of Streptococcus mutans [Tagashira et al, 1997;Furiga et al, 2008]. These modes of action are relevant for dental applications as proteins are constituents of dental plaque and as enzymes are responsible for plaque metabolism [Rosan and Lamont, 2000].…”

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confidence: 99%

Effect of Galla Chinensis on Growth and Metabolism of Microcosm Biofilms

et al. 2011

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Galla chinensis extract (GCE) interferes with de- and remineralization of dental enamel and the growth and metabolism in planktonic bacteria. However, no information is available on GCE effects on biofilms formed with saliva as inoculum. The aim of the current experiments was to investigate the effects of GCE at different stages of salivary microcosm biofilm formation. Biofilms formed on glass or enamel surfaces were treated with GCE solutions at different concentrations and at different time points. Effects were assessed by lactic acid formation and colony-forming unit (CFU) counts of the biofilms. The results showed that GCE treatments inhibited growth and acid metabolism of both nascent and mature microcosm biofilms. Pretreatment of the substratum with GCE solutions inhibited growth and lactic acid production of biofilms grown on enamel, but had little effects on biofilms formed on glass surfaces. A maximum GCE effect was found when biofilms, on either surface type, were treated after 8 h of formation with 40 h of subsequent growth. In medium with sucrose-fermenting biofilms, low concentrations of GCE (0.2 and 0.1 mg/ml) inhibited acid production without killing bacteria of the biofilm. Differences were found in GCE effects on biofilms formed with saliva from different donors, with reductions in acid formation and CFU values ranging between 0 and 78%. In conclusion, bioactive components in GCE reduce or inhibit both growth and lactic acid formation in biofilms.

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Effect of antiplaque compounds and mouthrinses on the activity of glucosyltransferases from Streptococcus sobrinus and insoluble glucan production

Abstract: As the formulation of commercial products considerably influences the efficiency of active components, the fast representative ISG inhibition test developed in this study should be of great interest.

Cited by 20 publications

References 58 publications

Preventive effects of an original combination of grape seed polyphenols with amine fluoride on dental biofilm formation and oxidative damage by oral bacteria

Preventive effects of an original combination of grape seed polyphenols with amine fluoride on dental biofilm formation and oxidative damage by oral bacteria

Biology of <i>Streptococcus mutans-</i>Derived Glucosyltransferases: Role in Extracellular Matrix Formation of Cariogenic Biofilms

Effect of Galla Chinensis on Growth and Metabolism of Microcosm Biofilms

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