Effect of Antioxidants on the Stored Dromedary Camel Epididymal Sperm Characteristics

El-Harairy, M.; El-Razek, Ibrahim M. Abd; Abdel-Khalek, A.; Shamiah, Sherif M.; Zaghloul, H. K.; Khalil, Wael A.

doi:10.3923/ajas.2016.147.153

Cited by 7 publications

(7 citation statements)

References 25 publications

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“…Similarly, in Murrah buffalo, caffeine significantly reduced abnormalities [43] . Most recent in in vitro trials on camel semen, epididymal camel spermatozoa diluent supplemented with antioxidants had a positive effect in maintaining the function and morphology as well as membrane integrity of sperm [44] .…”

Section: Discussionmentioning

confidence: 98%

The influence of caffeine supplementation and concerted utilization of enzymatic and mechanical semen liquefaction on freezability of dromedary camel spermatozoa

El-Bahrawy

2017

International Journal of Veterinary Science and Medicine

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Assisted reproductive technologies have been reported to improve reproductive efficiency and genetic potential in camelids. Two experiments were carried out to determine efficiency of centrifugation in the presence of a mucolytic agent for liquefaction of dromedary semen. In the first experiment, three groups, namely: I. Tris lactose (TL, control), II. Tris lactose supplemented with amylase (TL_A) and III. Tris lactose supplemented with amylase followed by seminal plasma removal via centrifugation (TL_A_Cent.) and re-suspension into enzyme free TL media. After equilibration, control group recorded 62.85 ± 4.28% motility and 1.3 ± 0.13 viscosity score, while TL_A group values were 72.88 ± 3.30% and 0.83 ± 0.07%, respectively. TL_A_Cent. group showed significant viscosity reduction (0.33 ± 0.05) and motility decline 47.85 ± 3.04% with increment in abnormalities and detached acrosome. The second experiment investigated the effect of caffeine addition to tolerate enzymatic and mechanical stress. Using 4 mM caffeine in amylase-treated semen (TL_AC) improved post-thaw motility 50.0 ± 1.29% and recovery rate 77.8 ± 3.83% compared to the control (40.17 ± 2.79% and 62.55 ± 8.39%), respectively. Caffeine supplemented centrifuged samples (TL_AC_Cent.) showed superiority (P < .05) in post-thaw motility and recovery rate (38.33 ± 6.41%, 62.76 ± 8.10%) compared to centrifuged samples TL_A_Cent. without caffeine addition (25.00 ± 2.88% and 40.47 ± 3.48%), respectively. Sperm kinetics showed that TL_A exhibited high (p < .05) values for mostly all sperm kinetics. Caffeine treatments showed superiority in velocity curved line (VCL, µm/s) 94.24 ± 8.44 for TL_AC and 104.25 ± 8.72 for the TL_AC_Cent. group compared to 86.8 ± 5.54 for TL_A, and 85.73 ± 5.99 for the TL_A_Cent. groups. In conclusion, preforming a combined enzymatic-mechanical protocol in the presence of an antioxidant may be crucial for refinement of camel semen cryopreservation.

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Section: Discussionmentioning

confidence: 98%

The influence of caffeine supplementation and concerted utilization of enzymatic and mechanical semen liquefaction on freezability of dromedary camel spermatozoa

El-Bahrawy

2017

International Journal of Veterinary Science and Medicine

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“…A study on epididymal sperm motility showed 42.8% for bulls aged between 37 -51 weeks and 55.9% for bulls aged between 52 -115 weeks old after 48 hours of storage of their epididymides at 5˚C [31]. Camel epididymal sperm diluted with tris-fructose extender supplemented with antioxidants were still motile (64%) after 48 hours of storage at 5˚C [24].…”

Section: Discussionmentioning

confidence: 99%

“…Epididymal spermatozoa may be more susceptible to oxidative stress than ejaculated spermatozoa since they are not exposed to seminal plasma which contains the complex secretions of the accessory sex glands which are regarded as the major source of antioxidant protection for spermatozoa [24].…”

Section: Discussionmentioning

confidence: 99%

Effect of Storage Time on Cauda Epididymal Sperm Parameters of Nigerian Local Dogs

Chima¹,

Abu²,

Dawuda³

et al. 2017

OJVM

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Aim: Storage of testes-epididymides at refrigeration temperature is a promising method of short-term sperm preservation for use in reproductive biotechnology in dogs. This study aimed to determine the effect of storage time at 4˚C on the quality of Nigerian local dogs' cauda epididymal spermatozoa recovered post-castration. Materials and Methods: Testes along with their epididymides collected immediately after castration of local dogs were either processed immediately (0th hour) or stored at 4˚C in the refrigerator for 12th, 24th or 48th hours and then processed. Each storage group comprised 5 pairs of testes-epididymides. Sperm motility, concentration, livability, acrosome integrity and morphology from the various storage groups were evaluated and means with standard error of the mean (±SEM) were recorded. Results: Mean percent sperm motility at 0th hour (77.0 ± 2.6) decreased significantly (p < 0.05) after 12 hours (68.0 ± 1.2), 24 hours (66.0 ± 2.4) and 48 hours (60.0 ± 1.6) of storage at 4˚C. No significant difference (p > 0.05) was found when the mean sperm concentrations (×10 7 /ml) of the storage groups (5.86 ± 47, 5.24 ± 26 and 5.38 ± 31) were compared with the control (6.48 ± 0.75). Mean percent sperm livability did not differ significantly (p > 0.05) between the 0th, 12th, 24th and 48th hour groups (86.4 ± 1.5, 78.0 ± 3.1 and 75.0 ± 1.8 respectively). However, there was a significant decrease (p < 0.05) in mean percent sperm livability after 48 hours of storage (69.8 ± 4.2) as compared with the 0th hour group. No significant difference (p > 0.05) was detected in the mean percent acrosome integrity between the 0th, 12th, 24th and 48th hour groups (90.0 ± 1.5, 83.0 ± 3.5, 84.6 ± 4.6 and 82.2 ± 3.9 respectively). A significant increase (p

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“…The extender was composed of 74 mL buffer + 6 mL glycerol + 20 mL egg yolk. The following supplements were added to the SHOTER extender to examine the effects on spermatozoa cryopreservation: Vit E (α tocopherol, T3251) at 200 µM/mL [55], Vit C (ascorbic acid, A4544) at 1 mg/mL [18], SeNPs at 1 µg/mL [47], ZnONPs at 50 µg/mL [20], Na 2 SeO 3 (Loba Chemie Pvt. Ltd., Mumbai, India) at 2 µg/mL [32], and ZnSO 4 (Loba Chemie) at 100 µM/mL [19].…”

Section: Experimental Designmentioning

confidence: 99%

“…In recent years, studies have also been conducted on camels, cow bulls, and human semen diluents, to supplement anti-oxidants such as glutathione, vitamin C (Vit C), vitamin E (Vit E), zinc (Zn), and selenium (Se) to improve the post-thawed motility, vitality, and membrane integrity of spermatozoa [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Comparison between the Effects of Adding Vitamins, Trace Elements, and Nanoparticles to SHOTOR Extender on the Cryopreservation of Dromedary Camel Epididymal Spermatozoa

Shahin

Khalil

Saadeldin

et al. 2020

Animals

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There are several obstacles in camel semen cryopreservation; such as increasing semen viscosity and the reduction in motile spermatozoa after ejaculation. Epididymal spermatozoa offer an efficient alternative to overcome these problems and are well-suited for artificial insemination in camels. In the current study, we compared the effects of supplementation with vitamin C, E, inorganic trace elements of selenium (Na2SeO3) and zinc (ZnSO4), and zinc and selenium nanoparticles (ZnONPs and SeNPs, respectively) on the cryopreservation of dromedary camel epididymal spermatozoa. When the SHOTOR extender was supplemented with ZnONPs and SeNPs; the sperm showed increased progressive motility; vitality; and membrane integrity after cooling at 5 °C for 2 h; when compared to the control and vitamin-supplemented groups. Moreover, the ZnONPs and SeNPs supplementation improved the progressive motility, vitality, sperm membrane integrity, ultrastructural morphology, and decreased apoptosis when frozen and thawed. SeNPs significantly increased reduced glutathione (GSH), superoxide dismutase (SOD), and decreased lipid peroxide malondialdehyde (MDA) levels. The advantageous effects of the trace elements were potentiated by reduction into a nano-sized particle, which could increase bioavailability and reduce the undesired liberation of toxic concentrations. We recommend the inclusion of SeNPs or ZnONPs to SHOTOR extenders to improve the cryotolerance of camel epididymal spermatozoa.

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Effect of Antioxidants on the Stored Dromedary Camel Epididymal Sperm Characteristics

Cited by 7 publications

References 25 publications

The influence of caffeine supplementation and concerted utilization of enzymatic and mechanical semen liquefaction on freezability of dromedary camel spermatozoa

The influence of caffeine supplementation and concerted utilization of enzymatic and mechanical semen liquefaction on freezability of dromedary camel spermatozoa

Effect of Storage Time on Cauda Epididymal Sperm Parameters of Nigerian Local Dogs

Comparison between the Effects of Adding Vitamins, Trace Elements, and Nanoparticles to SHOTOR Extender on the Cryopreservation of Dromedary Camel Epididymal Spermatozoa

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