Effect of antioxidants and antibiotics on levels of seminal oxidative stress in leukocytospermic infertile men

Abstract

Defective sperm function is the most common cause of infertility. A prospective study was carried out to correlate the concentration of nitrite (the stable metabolite of nitric oxide) in seminal plasma with leukocytospermia, and sperm membrane integrity. Total Fifty-seven normozoospermic subjects with and without leukocytospermia visiting the Infertility clinic at KH and MRC, Karad, were included in the present study. Semen samples were checked for sperm concentration, total sperm count, sperm motility, seminal leukocyte concentration and sperm membrane integrity as Hypoosmotic Swelling Test. Similarly the concentration of nitrite in seminal plasma was measured by Griess reaction and total antioxidant power measured as ferric reducing ability of plasma. The concentration of nitrite in seminal plasma was found to be raised with significantly increased leukocyte concentration in semen. Also significantly lowered levels of total antioxidant power along with defective sperm function was observed. Our results suggest that supplementary treatment of antioxidants with antibiotic for leukocytospermic infertile male patients may improve the sperm membrane integrity.

Potentiometric Method for Evaluating the Oxidant/Antioxidant Activity of Seminal and Follicular Fluids and Clinical Significance of this Parameter for Human Reproductive Function

“…Possibility of oxidant activity ( ) detection follows from the data obtained in our previous work [29], where interaction of potassium ferrocyanide K 4 [Fe(CN) 6 ] with peroxyl radicals was studied.…”

“…An increase in the potential of the mediator system (from the initial one) is observed followed by a fall. This dependence of the potential shift of the ferricyanide/ferrocyanide mediator system over time is due to the interaction of oxidants with ferrocyanide K 4 …”

“…Using ferricyanide/ferrocyanide mediator system containing 0,01M K 3 [Fe (CN) 6 ] and 0.0001MK 4 [Fe (CN) 6 ] provides the possibility to obtain shift of potential about 20-60 mV.…”

“…Thus, the presence of oxidative stress may lead to idiopathic forms of male sterility. Moreover, excessive ROS can cause induced apoptosis of sperm, and damage their DNA [4]. Breakages in DNA are a key indicator of cell death [4].…”

Toxic effect of Trichomonas vaginalis secretory products on human sperm in vitro

“…Клиниче-ские наблюдения показали высокую корреляцию результатов HOS-теста и ВРТ (искусственная инсе-минация) [36], а также корреляцию результатов HOS-теста и прогноза развития привычного невына-шивания беременности [37], что подтверждает зна-чимость этого исследования при оценке фертильно-сти сперматозоидов. Также HOS-тест показал высо-кую информативность при оценке фертильности сперматозоидов в экспериментальных условиях, мо-делирующих бактериальную контаминацию спермы [38] и различные токсические воздействия [39,40].…”