Potentiometric method based on the sharp change of electrode potential for the ferricyanide/ferrocyanide mediator system after addition of the seminal or follicular fluid sample into the measuring electrochemical cell has been developed to enable the easy assessment of the oxidant/antioxidant status of these kind of biological fluids. Platinum screen printed electrode has been used as an indicator. Essential advantage of the proposed method is simultaneous use of the both forms of the ferricyanide/ferrocyanide mediator system (oxidized and reduced ones) that allows estimating oxidant and antioxidant components of the seminal or follicular fluids samples which were obtained, respectively, from 99 male and 27 female subjects. Oxidant activity of seminal fluids has been observed in pathology conditions of reproductive system of male studied subjects and related with possible male infertility symptoms.
The new standing order of Russian Ministry of Health for the use of assisted reproductive technologies (ART) (in force since 2013) permits the use of ART for discordant couples with one partner infected with HIV. This permission puts on clinics an additional responsibility due to risks of HIV transmission. With the aim to decrease the possibility of nosocomial HIV infection of mother and child, the method of HIV detection in washed sperm was developed and validated.
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