Effect of antigen challenge on the activation of peripheral blood neutrophils from horses with chronic obstructive pulmonary disease

Marr, K.A.; Foster, A. P.; Lees, P.; Cunningham, Fiona; Page, Clive

doi:10.1016/s0034-5288(97)90200-9

Cited by 21 publications

(14 citation statements)

References 20 publications

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“…In asthmatic patients, systemic oxidative stress is explained as a result of increased superoxide generation by peripheral neutrophils . In RAO in horses, neutrophils circulating in the blood have also been shown to produce increased amounts of superoxide anions during exposure to organic dust . Thus, it is possible that a systemic imbalance in oxidant‐antioxidant status in RAO‐affected horses may be a consequence of the disease and activation of peripheral neutrophils.…”

Section: Discussionmentioning

confidence: 99%

Oxidant‐Antioxidant Status in the Blood of Horses with Symptomatic Recurrent Airway Obstruction (RAO)

Niedźwiedź

Jaworski

2014

Veterinary Internal Medicne

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BackgroundSystemic oxidative stress in horses with recurrent airway obstruction (RAO) is poorly characterized.ObjectivesThe goal of this study was to investigate whether equine RAO is associated with systemic disturbances in the oxidant‐antioxidant equilibrium.AnimalsSeven healthy horses and 7 horses with symptomatic RAO.MethodsA prospective study. Healthy and RAO‐affected horses were exposed to a 48‐hour challenge with moldy hay and straw to induce clinical exacerbation of RAO. Venous blood was collected and the activities of the superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in equine erythrocyte lysates were measured. The concentration of thiobarbituric acid‐reactive substances (TBARSs) was assessed both in erythrocyte lysates and in plasma.ResultsA significant increase in the activities of GPx and SOD was detected in RAO‐affected horses compared with the control animals. There was no significant difference between groups in terms of the erythrocyte lysate activities of CAT, GR, or TBARs or the plasma concentration of TBARs.Conclusion and Clinical ImportanceOur results support the hypothesis that RAO in horses is associated with systemic oxidative stress. Future studies are needed to assess whether horses suffering from RAO can benefit from antioxidant supplementation.

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Section: Discussionmentioning

confidence: 99%

Oxidant‐Antioxidant Status in the Blood of Horses with Symptomatic Recurrent Airway Obstruction (RAO)

Niedźwiedź

Jaworski

2014

Veterinary Internal Medicne

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“…For this reason, neutrophil func- tion in RAO-affected horses has been a major focus of investigation. Results of studies [17][18][19][20] to investigate the production of reactive oxygen species and neutrophil adhesion in horses have indicated that the type of stimulus and type of functional response measured appear to be important in determining whether differences between RAO-affected and control horses will be detected. The study reported here was intended to investigate production of LTB 4 (an important chemotactic agent for neutrophils) by neutrophils in peripheral blood and BALF samples from RAO-affected and control horses before and during exposure to hay or straw.…”

Section: Discussionmentioning

confidence: 99%

Assessment of leukotriene B4 production in leukocytes from horses with recurrent airway obstruction

Lindberg

Robinson²,

Näsman-Glaser³

et al. 2004

ajvr

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“…There is a modest but significant increase in peripheral blood neutrophil 7–24 h following the initiation of stable dust exposure 76,125,126 . Blood neutrophils also have an increased active respiratory burst and delayed spontaneous apoptosis 24 h after challenge 145–147 . Increased CD18‐dependant adherence is present, even during clinical remission, suggesting a persistent state of neutrophil activation 145 .…”

Section: Systemic Inflammationmentioning

confidence: 99%

Heaves, an asthma‐like disease of horses

Leclère¹,

Lavoie-Lamoureux²,

Lavoie³

2011

Respirology

176

233

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Animal models have been developed to investigate specific components of asthmatic airway inflammation, hyper-responsiveness or remodelling. However, all of these aspects are rarely observed in the same animal. Heaves is a naturally occurring disease of horses that combines these features. It is characterized by stable dust-induced inflammation, bronchospasm and remodelling. The evaluation of horses during well-controlled natural antigen exposure and avoidance in experimental settings allows the study of disease mechanisms in the asymptomatic and symptomatic stages, an approach rarely feasible in humans. Also, the disease can be followed over several years to observe the cumulative effect of repeated episodes of clinical exacerbation or to evaluate long-term treatment, contrasting most murine asthma models. This model has shown complex gene and environment interactions, the involvement of both innate and adaptive responses to inflammation, and the contribution of bronchospasm and tissue remodelling to airway obstruction, all occurring in a natural setting. Similarities with the human asthmatic airways are well described and the model is currently being used to evaluate airway remodelling and its reversibility in ways that are not possible in people for ethical reasons. Tools including antibodies, recombinant proteins or gene arrays, as well as methods for sampling tissues and assessing lung function in the horse are constantly evolving to facilitate the study of this animal model. Research perspectives that can be relevant to asthma include the role of neutrophils in airway inflammation and their response to corticosteroids, systemic response to pulmonary inflammation, and maintaining athletic capacities with early intervention.

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Effect of antigen challenge on the activation of peripheral blood neutrophils from horses with chronic obstructive pulmonary disease

Cited by 21 publications

References 20 publications

Oxidant‐Antioxidant Status in the Blood of Horses with Symptomatic Recurrent Airway Obstruction (RAO)

Oxidant‐Antioxidant Status in the Blood of Horses with Symptomatic Recurrent Airway Obstruction (RAO)

Assessment of leukotriene B4 production in leukocytes from horses with recurrent airway obstruction

Heaves, an asthma‐like disease of horses

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