Effect of anti-sca-1+ on diabetic rat histological, immunohistochemical and biochemical evaluation.

Abstract

Diabetes mellitus is a disease of metabolic dysregulation most notable glucose metabolism accompanied by long term complications. The present study was carried out to investigate the potential effect of Anti-Sca-1 + stem cells on type 1 diabetes induced in albino rats. The study was carried on 7 male and 24 female Albino rats (150 ± 5g). The female albino rats were divided into three main groups: normal control, diabetic group and diabetic rats treated by Anti-Sca-1 +. Induction of diabetic rats was carried out by single dose injection of fasting rats for 48 h with a diluted solution of 40 mg/kg of alloxan monohydrate (2%) in freshly prepared saline solution. Diabetic rats treated group was slaughtered into two phases; the first phase after 15 days of treatment, and the second phase after 30 days of treatment. Tissue samples were separated and kept in absolute 10% formalin solution for histological and immunohistochemical studies. SRY gene expression and biochemical study of glucose tolerance, c-peptide, insulin, glycated hemoglobin and glucose transporter 2 was evaluated. The results showed that, treatment of diabetic rats, with derivative bone marrow (Anti-Sca-1 +) could greatly ameliorate glucose, insulin, c-peptide, HbA1c and glut2, Hafter 15 and 30 days of treatment, as compared to normal control group and these were confirmed by histological and immunological investigations, which indicate significant progress in the pancreas tissue, by marked significant increase of pancreatic islets size and improvement in the acinar cells after 30 days of treatment by Anti-Sca-1 + .