Effect of anti-inflammatory agents on the haemorrhagic response of mouse skin to Bordetella pertussis heat-labile toxin

Differentiation of phase I and variant strains of Bordetella pertussis on Congo red media

“…Heat-labile toxin activity was determined by skin reactions after subcutaneous injection of 3-4-week-old mice (Parton, 1986). Mice received injections of 0-05-ml amounts of the bacterial suspensions diluted to 2 OU in PBS.…”

Purification and characterization of the heat-labile toxin of Bordetella pertussis

“…Pertussis toxin is resistant to treatment at 560C and acts through ADP-ribosylation of adenylate cyclase regulatory protein Gi (4,15). The lethal action of PEHLT can be inhibited by giving animals corticosteroids at the time of toxin challenge (27,28), but detailed information describing the biochemical basis for the action of PEHLT is unavailable.…”

Inhibition of heat-labile toxin from Bordetella parapertussis by fatty acids

“…Intradermal injection of HLT into suckling mice results in hemorrhagic lesions, whereas adult mice are refractory to this action of the toxin (14,18). Adult mice, however, are susceptible to other actions of HLT (16).…”

“…HLT acts on the skin of a number of animal species, including mice, rats, guinea pigs, rabbits, chicken, sheep, and pigs (13,15,18). The type of skin lesion induced by HLT depends on the animal species.…”

“…In mice, the potency of the toxin depends on the age of the animal. For example, HLT induces hemorrhagic necrosis in the skin of suckling mice, whereas the toxin has no effect on the skin of adult mice (18). These differences in responsiveness of mice to HLT action could be due to the presence of a factor(s) in the skin which affects HLT activity.…”