Effect of ancient Khorasan wheat on gut microbiota, inflammation, and short-chain fatty acid production in patients with fibromyalgia

Baldi, Simone; Pagliai, Giuditta; Dinu, Monica; Gloria, Leandro Di; Nannini, Giulia; Curini, Lavinia; Pallecchi, Marco; Russo, Edda; Niccolai, Elena; Danza, Giovanna; Benedettelli, Stefano; Ballerini, Giovanna; Colombini, Barbara; Bartolucci, Gianluca; Ramazzotti, Matteo; Sofi, Francesco; Amedei, Amedeo

doi:10.3748/wjg.v28.i18.1965

Cited by 11 publications

(9 citation statements)

References 45 publications

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“…We also found some rare ora, such as Cyanobacteria, Deinococcus_Thermus, Synergistetes, and Candidatus_Saccharibacteria. Some of these ora are ingested through food or water sources [16]; others are intestinal colonizing bacteria, which researchers have found in the human gastrointestinal tract [17][18][19]. In this study, antibiotics inhibited Bacteroidetes, Proteobacteria, Verrucomicrobia, Synergistetes, and Candidatus Saccharibacteria; meanwhile, Firmicutes, Actinobacteria, Fusobacteria, Cyanobacteria, and Deinococcus_Thermus exhibited increased abundances.…”

Section: Discussionmentioning

confidence: 64%

Alteration of the intestinal flora in children after antibiotic treatment explored using 16S rRNA sequencing

zhou

2024

Preprint

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Background During the critical period of intestinal flora establishment, children are infected, and their flora types and numbers are unstable and vulnerable to environmental influences. Antibiotic therapy is one of the leading causes of intestinal flora dysbiosis in children, but the underlying mechanism is still unclear. In this study, we investigated the changes in the characteristics of the intestinal flora in children after antibiotic treatment from a microscopic perspective via 16S rRNA sequencing technology, which can help clarify the effects of antibiotics on children's intestinal flora and provide a basis for the diagnosis and treatment of antibiotic-related diseases. Methods Fifty-seven children with respiratory infectious diseases were recruited for this study. The patients were divided into a study group (AG) and a control group (NG) according to whether they were receiving antibiotics. Three different antibiotics were used in the AG group (28 patients): Meloxicillin Sulbactam (Suzhou Erye Pharmaceutical Co., Ltd.), Ceftriaxone (Shenzhen Lizhi Pharmaceutical Co., Ltd.), and Azithromycin (Hainan Puri Pharmaceutical Co., Ltd.). The duration of antibiotic treatment was 3-5 days. The NG group (n=29) included children who were not treated with antibiotics. We collected a total of 57 stool specimens. These specimens were subsequently analyzed via 16S rRNA gene sequencing. Continuous nonparametric data are represented as medians and were analyzed using the Wilcoxon rank-sum test. Results Diversity analysis revealed a significant increase in the mean abundance but a significant decrease in diversity and a significant change in the composition of the intestinal flora in children after antibiotic treatment. At the phylum level, the intestinal flora of children consisted mainly of Bacteroidetes, Firmicutes, Proteobacteria, Verrucomicrobia, Actinobacteria, and Fusobacteria. At the genus level, antibiotic treatment significantly inhibited 33 groups of intestinal flora, such as Prevotella, while substantially increasing the number of 13 groups of intestinal flora, such as Megamonas and Enterococcus. In terms of metabolic function, protein modification, cofactors, prosthetic groups, electron carriers, vitamin biosynthesis, nucleic acid processing, secondary metabolite biosynthesis, and aldehyde degradation were significantly decreased. In contrast, the carbohydrate degradation and pentose phosphate pathway functions increased considerably after antibiotic treatment. Conclusions Antibiotics have a significant impact on the composition and diversity of the intestinal flora of children. Antibiotics inhibited the growth of some colonizing flora, leading to an overgrowth of opportunistic pathogenic bacteria, which resulted in significant changes in some metabolic functions. Moreover, we identified a series of flora with considerable variability at the genus level, and these alterations in the intestinal flora could provide an additional basis for the diagnosis and treatment of antibiotic-associated diseases.

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Section: Discussionmentioning

confidence: 64%

Alteration of the intestinal flora in children after antibiotic treatment explored using 16S rRNA sequencing

zhou

2024

Preprint

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“…In addition to the Mediterranean diet, some other diet regimens can also improve GM and OS. For example, the replacement diet based on ancient Khorasan wheat results in beneficial GM compositional and functional modifications that positively correlate with an improvement in fibromyalgia symptomatology[ 59 ]. A habitual diet supplemented with oral butyrate could reduce the production of ROS in leukocytes[ 60 ].…”

Section: Potential Interventions For Frailtymentioning

confidence: 99%

Oxidative stress bridges the gut microbiota and the occurrence of frailty syndrome

Chen¹,

Wang²,

Zhao³

et al. 2022

World J Gastroenterol

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The incidence of frailty gradually increases with age. This condition places a heavy burden on modern society, of which the aging population is increasing. Frailty is one of the most complicated clinical syndromes; thus, it is difficult to uncover its underlying mechanisms. Oxidative stress (OS) is involved in frailty in multiple ways. The association between the gut microbiota (GM) and frailty was recently reported. Herein, we propose that OS is involved in the association between the GM and the occurrence of frailty syndrome. An imbalance between oxidation and antioxidants can eventually lead to frailty, and the GM probably participates in this process through the production of reactive oxygen species. On the other hand, OS can disturb the GM. Such dysbiosis consequently induces or exacerbates tissue damage, leading to the occurrence of frailty syndrome. Finally, we discuss the possibility of improving frailty by intervening in the vicious cycle between the imbalance of OS and dysbiosis.

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“…Nesse sentido, pacientes com sintomas gastrointestinais que foram alvo de intervenções nutricionais como dieta vegetariana, à base de oligossacarídeos de baixa fermentação, além de suplementação de grãos, apresentaram resposta significativa na melhora desses sintomas. Em especial uma dieta de substituição com o trigo Khorasan tem a capacidade de produzir efeitos benéficos relacionados à uma melhora geral desse tipo específico de sintoma uma vez que a dieta resulta em modificações funcionais e de composição da microbiota intestinal que são benéficas ao paciente com fibromialgia (Baldi et al, 2022).…”

Section: Terapêutica Dietéticaunclassified

Avanços na abordagem terapêutica da fibromialgia: uma revisão integrativa

Souza

Silva

Pereira

et al. 2022

RSD

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A fibromialgia (FM) é uma síndrome caracterizada como um distúrbio sistêmico complexo, a qual afeta milhões de pessoas em todo o mundo, levando ao aumento do uso de terapias não farmacológicas, como medidas terapêuticas com menos efeitos colaterais e com o intuito de uma abordagem mais efetiva da FM. O presente estudo de revisão buscou avaliar novos avanços na abordagem terapêutica da fibromialgia, documentados por meio de estudos clínicos e randomizados. Trata-se de uma pesquisa de revisão integrativa realizada por meio da base de dados PubMed, que levou em consideração os seguintes critérios de inclusão: ensaios clínicos e testes controlados e aleatórios; artigos publicados no último ano que possuíam texto completo disponível e que abordassem o tratamento da fibromialgia. Foi observado que a prática de fortalecimento muscular central, alongamento e de vibração do corpo inteiro se mostrou benéfica na melhora da qualidade de vida, assim como intervenções em grupo que possuem enfoque no autocuidado e na educação em neurociência da dor. Terapias intervencionistas como a estimulação transcraniana por corrente contínua demonstraram divergências quanto à eficácia do método, e uma dieta de substituição com o trigo Khorasan resultou em melhora geral dos sintomas gastrointestinais vistos em pacientes com fibromialgia. Espironolactona e alopurinol se mostraram ineficazes, diferente da suplementação de vitamina B6 e magnésio, ambas relacionadas com melhora da dor e redução do estresse, respectivamente, em pacientes com fibromialgia. Dessa forma, novos estudos que possibilitem a compreensão efetiva de tais modalidades terapêuticas no manejo da fibromialgia são sugeridos pelos autores.

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Effect of ancient Khorasan wheat on gut microbiota, inflammation, and short-chain fatty acid production in patients with fibromyalgia

Cited by 11 publications

References 45 publications

Alteration of the intestinal flora in children after antibiotic treatment explored using 16S rRNA sequencing

Alteration of the intestinal flora in children after antibiotic treatment explored using 16S rRNA sequencing

Oxidative stress bridges the gut microbiota and the occurrence of frailty syndrome

Avanços na abordagem terapêutica da fibromialgia: uma revisão integrativa

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