Effect of an extract of cauliflower (leaf) on the labeling of blood elements with technetium-99m and on the survival of Escherichia coli AB1157 submitted to the treatment with stannous chloride

Lima, Elaine A. C.; ., G.F. Diré; Mattos, D. M. M.; Freitas, Renata de; Gomes, M. L.; Oliveira, Mariana Borges; Faria, Mauro Velho Castro; Jales, Roberto Levi Cavalcanti; Bernardo-Filho, Mário

doi:10.1016/s0278-6915(02)00065-0

Cited by 31 publications

(24 citation statements)

References 23 publications

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“…One of these studies was performed by using cauliflower which is in the same family with broccoli. In this study, E. coli AB1157 strain was treated with SnCl 2 in the presence of cauliflower and it abolished the lethal effect of SnCl 2 on the E. coli strains 6 .…”

Section: Resultsmentioning

confidence: 93%

“…Following 24h incubation at 35ºC, the total number of bacteria for each dilution was counted and colony-forming units per milliliter (cfu/mL) were determined. The survival fraction (N/N 0 ) was calculated as previously described 6 .…”

Section: Thin Layer Chromatography (Tlc)mentioning

confidence: 99%

“…Since stannous dichloride (SnCl 2 ) is the most common reducing agent in 99m Tc radiopharmaceuticals preparation, it has been shown that severe biological effects on central nervous system and oral mucosa in animal studies 1,4 . Also it has been reported that SnCl 2 had lethal effect on Escherichia coli (E. coli) with cytotoxic and genotoxic effects [5][6][7][8] . Genotoxic effect of SnCl 2 may be directly on deoxyribonucleic acid (DNA) or indirectly by generating free radicals 9 .…”

Section: Introductionmentioning

confidence: 99%

“…In recent studies, some authors have also described that genotoxic and cytotoxic effects of SnCl 2 can be altered by herbal extract, chemicals and pharmaceutics on E. coli 5,6,[8][9][10][11][12][13] .…”

Section: Introductionmentioning

confidence: 99%

“…Lima et al 6 demonstrated that cauliflower from Brassicacea family was abolished the lethal effect of SnCl 2 on the E. coli strains.…”

Section: Introductionmentioning

confidence: 99%

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Anti toxic effect of broccoli extract on stannous dichloride toxicity

et al. 2012

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PURPOSE: Since Technetium-99m ( 99m Tc) has favorable physical and chemical characteristics, it is widely used radioisotope in Nuclear Medicine. However, stannous dichloride (SnCl 2 ) has been widely used as a reducing agent in labeling procedure of pharmaceutical with radionuclide, it has been realized that SnCl 2 have genotoxic and cytotoxic effects on biological systems. In previous studies, it has been shown that some herbal extract can reduce genotoxic and cytotoxic effects of SnCl 2 . In the present study, it is aimed to evaluate the effect of the broccoli extract on the survival of E. coli ATCC 25922 strain against to toxic effects of SnCl 2 . METHODS:Broccoli was extracted with methanol extraction. HPLC and TLC analysis of broccoli extract were performed. Then antitoxicity and dose response assays were performed on bacterial strain. RESULTS:The broccoli extract had dose dependent protective effect against SnCl 2 toxic effect on E. coli. CONCLUSIONS:The consumption of broccoli may alter the stannous dichloride toxicity. Broccoli extract may use as a new protective strategies against the toxic effect of SnCl 2 on patients who were taken 99m Tc radiopharmaceuticals. Key words: Broccoli. Stannous dichloride. Technetium-99m. Escherichia coli. Toxicity. RESUMOOBJETIVO: Em face de suas características físico-químicas, o Tecnécio-99m ( 99m Tc) é um radiofármaco amplamente utilizado na Medicina Nuclear. Todavia, o dicloreto de estanho (SnCl 2 ) tem sido largamente aplicado como um agente redutor no procedimento farmacêutico de marcação com radionuclídeos. Constatou-se que o SnCl 2 apresenta efeitos genotóxicos e citotóxicos nos sistemas biológicos. Em estudos prévios, foi demonstrado que alguns extratos de ervas podem reduzir tais efeitos. O estudo atual objetivou avaliar os efeitos do extrato de brócolis na sobrevida da cepa E. coli ATCC 25922, exposta ao efeito tóxico do SnCl 2.MÉTODOS: O extrato de brócolis foi obtido mediante extração com metanol. Analises com HPLC e TLC foram efetuadas. Avaliou-se a antitoxicidade e realizou-se um ensaio dose-resposta para uma cepa de bactérias. RESULTADOS:O extrato de brócolis mostrou um efeito protetor dose dependente para os efeitos tóxicos do SnCl 2 sobre a E. coli. CONCLUSÕES:O consumo de brócolis pode alterar a toxicidade do dicloreto de estanho . O extrato de brócolis pode ser utilizado como uma nova estratégia para proteção de pacientes contra os efeitos tóxicos do SnCl 2, nos quais foi administrado o radiofármaco Tecnécio-99m.Descritores: Brassica. Compostos Estanho. Tecnécio. Escherichia coli. Toxicidade. Anti toxic effect of broccoli extract on stannous dichloride toxicity

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Section: Resultsmentioning

confidence: 93%

Section: Thin Layer Chromatography (Tlc)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Lima et al 6 demonstrated that cauliflower from Brassicacea family was abolished the lethal effect of SnCl 2 on the E. coli strains.…”

Section: Introductionmentioning

confidence: 99%

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Anti toxic effect of broccoli extract on stannous dichloride toxicity

et al. 2012

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Brassica oleracea (Botrytis Group)

Lim¹

2013

Edible Medicinal and Non-Medicinal Plants

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Aqueous extract of the medicinal plant Mentha crispa alters the biodistribution of the radiopharmaceutical sodium pertechnetate in Wistar rats

et al. 2007

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Mentha crispa extract or 0.9% NaCl were (15 days) administered daily by gavage to Wistar rats. Animals then received Na 99m TcO 4 via ocular plexus and were sacrificed. Organs were isolated, radioactivity was determined, percentages of radioactivity in each organ was calculated, and statistical analyses performed. Significant (p = 0.0061) increases of radioactivity were observed in kidney, spleen, and thyroid, which could be exampled by the effects of the extract and/or by the generation of active metabolites.

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Effect of an extract of cauliflower (leaf) on the labeling of blood elements with technetium-99m and on the survival of Escherichia coli AB1157 submitted to the treatment with stannous chloride

Cited by 31 publications

References 23 publications

Anti toxic effect of broccoli extract on stannous dichloride toxicity

Anti toxic effect of broccoli extract on stannous dichloride toxicity

Brassica oleracea (Botrytis Group)

Aqueous extract of the medicinal plant Mentha crispa alters the biodistribution of the radiopharmaceutical sodium pertechnetate in Wistar rats

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