Effect of an Albumin-Coated Mesoporous Silicon Nanoparticle Platform for Paclitaxel Delivery in Human Lung Cancer Cell Line A549

Abstract

Albumin-coated paclitaxel-mesoporous silicon nanoparticles (APMSN) were prepared to improve the anticancer effect in lung cancer by means of regulating the dissolution rate of paclitaxel (PTX). PTX was absorbed into the mesoporous structure of mesoporous silicon nanoparticles (MSN), which was defined as PMSN. PTX was proved to exist in an amorphous state in PMSN, which increased the dissolution rate of PTX. Albumin was coated on the surface of MSN to form AMSN; AMSN and PTX were mixed to form APMSN in order to achieve sustained release of PTX. Then, it was found that APMSN had more significant antiproliferate effects and induced more apoptotic proportion in comparison with PTX in A549 cells. Furthermore, the absorption mechanism of APMSN into A549 cells was investigated. Transmission electron microscopy (TEM) and laser scanning confocal microscopy (LSCM) showed that APMSN could cross the cell membrane and was taken into the cytoplasm quickly. Taken together, our results demonstrate that AMSN carriers have potential as nanodrug delivery systems in the treatment of lung cancer.

Natural Biopolymers as Smart Coating Materials of Mesoporous Silica Nanoparticles for Drug Delivery

“…At pH 7.4, an albumin molecule has more than 200 negative charges, whereas the MSNs with amino groups are positively charged. Due to electrostatic interactions, the pores of MSNs can be sealed by albumin [ 263 ]. Karamana et al used different surface-modified MSN-PEI/PEG to study the interaction mechanism of HSA with MSN analogs.…”

“…Because of all of the above-mentioned characteristics, albumin coating has been combined with other molecules or synthetic polymers in order to obtain effective drug delivery systems with MSNs as nanocarriers [ 158 , 259 , 267 ]. Most of them have been studied for the transport of anticancer drugs with poor pharmacokinetic profile and metabolic stability such as DOX, PTX, and DTX, among others [ 263 , 268 ].…”

Revising Protein Corona Characterization and Combining ITC and Nano-DSC to Understand the Interaction of Proteins With Porous Nanoparticles

“…However, despite the extensive studies and the known influence of the NP physicochemical properties in the formation of the PC, only a few reports pay attention and focus on the influence of the NP roughness and pores in the formation of the PC, which is one of the keys in the final physiological response of the nanoparticle. ( Ma et al, 2014 ; Gao et al, 2016 ).…”

Albumin-Coated Mesoporous Silica Nanoparticles of Docetaxel: Preparation, Characterization, and Pharmacokinetic Evaluation

“…Coating of MSNs with albumin, an endogenous human protein, may increase the drug diffusion resistance and a decreased dissolution rate to obtain sustained drug release profile. 23 This leads to the need for development of albumincoated MSNs to modulate drug release for prolonged time. The stimuli-responsive biodegradable MSNs coupled with bovine serum albumin (BSA) and folic acid showed an ''on-demand'' targeted release of epirubicin for cancer treatment.…”